Functional Analysis of the c-myb Proto-Oncogene
The v-myb gene was initially identified as the oncogenic agent in the defective avian myeloblastosis virus (AMV), which induces acute myeloblasts leukemia in vivo and transforms myeloid cells in vitro (1). Klempnauer et al. (2,3) showed that the v-myb portion of AMV was transduced from seven internal exons of a cellular gene designated c-myb. A second replication-defective avian retrovirus, E26, was also found to contain v-myb as well as v-ets sequences (4,5). As with AMV, E26 contains a v-myb component which is an altered version of c-myb, with each containing both amino- and carboxy-terminal truncations of their gene products. The proteins encoded by AMV, E26, and c-myb have been localized to the nucleus (6,7).
KeywordsNorthern Blot Analysis Fetal Liver Hematopoietic Progenitor Partial cDNAs Transcriptional Activation Domain
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