Silicone Gel and Octamethylcyclotetrasiloxane Potentiate Antibody Production to Bovine Serum Albumin in Mice
Earlier studies, in the rat, have shown that silicone gel acts as a potent adjuvant for antibody production against bovine serum albumin (BSA). The purpose of the present study is to determine whether components of the silicone breast implant (silicone oil, silicone gel, and octamethylcyclotetrasiloxane [D4]) potentiate the antibody response to BSA in mice. Groups of ten A/J mice received a single subcutaneous injection of 0.1 ml of BSA emulsified with the following: a) silicone preparations, b) incomplete Freund’s adjuvant, or c) saline alone. Antibodies to BSA were measured using an enzyme-linked immunosorbant assay. The results indicate that both D4 and the silicone gel potentiate antibody production to BSA in mice. Known adjuvants have been shown to induce autoimmune syndromes in animal models. Whether silicones can act in a similar mechanism is still unclear.
KeywordsArthritis Albumin Bacillus Paraffin Streptomyces
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- 1.Dr. Rose has served as expert witness on behalf of defendants in several legal proceedings involving silicone breast implants. He has also served as consultant for Mentor Corporation, a manufacturer of breast implants.Google Scholar
- 5.Miyoshi K, Miyaoki T, Kobayashi Y, et al. (1962) Hypergammaglobulinemia by prolonged adjuvanticity in man: disorders developed after augmentation mammoplasty. Jap Med J: 2122, 9–14Google Scholar
- 6.Miyoshi K, Miyaoki T, Kobayashi Y (1973) Adjuvant disease in man. Clin Immunol 5:785–794Google Scholar
- 7.Pearson CM (1956) Development of arthritis, periarthritis, and periostitis in rats given adjuvants. Proc Soc Exp Med 91:95–100Google Scholar
- 10.Wilkerson P (1992) Report presented before the General and Plastic Surgery Device Evaluation Panel, FDA, Bethesda, MD, February 1992Google Scholar
- 11.Roitt IM (1994) Essential immunology. Blackwell Scientific Publications, BostonGoogle Scholar
- 12.Le Vier RR. Discussion of: Semple JL, Narini PP, Hay JB (193) Preparation techniques for silicone gel in the evaluation of its in vivo effects. Plast Reconstr Surg 92(5):979–81Google Scholar
- 13.Rose NR, Freidman H, Fahey JL, Eds (1986) Manual of clinical laboratory immunology, 3rd Ed. American Society for Microbiology, Washington, D.C.Google Scholar
- 14.Ratkay LG, Zhang L, Tonzetich J, Waterfield JD (1993) Complete Freund’s adjuvantinduces an earlier and more severe arthritis in MRL-lpr mice. Journal of Immunol 151(9):5081–7Google Scholar
- 18.Whitehouse MW, Orr KJ, Beck FWJ, Pearson CM (1974) Freund’s adjuvants: relationship of arthritogenicity and adjuvanticity in rats to vehicle composition. Immunol 27:311Google Scholar