Silicone Gel and Octamethylcyclotetrasiloxane Potentiate Antibody Production to Bovine Serum Albumin in Mice

  • J. J. NicholsonIII
  • G. E. Wong
  • C. G. Frondoza
  • N. R. Rose
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 210)


Earlier studies, in the rat, have shown that silicone gel acts as a potent adjuvant for antibody production against bovine serum albumin (BSA). The purpose of the present study is to determine whether components of the silicone breast implant (silicone oil, silicone gel, and octamethylcyclotetrasiloxane [D4]) potentiate the antibody response to BSA in mice. Groups of ten A/J mice received a single subcutaneous injection of 0.1 ml of BSA emulsified with the following: a) silicone preparations, b) incomplete Freund’s adjuvant, or c) saline alone. Antibodies to BSA were measured using an enzyme-linked immunosorbant assay. The results indicate that both D4 and the silicone gel potentiate antibody production to BSA in mice. Known adjuvants have been shown to induce autoimmune syndromes in animal models. Whether silicones can act in a similar mechanism is still unclear.


Bovine Serum Albumin Adjuvant Arthritis Autoimmune Syndrome Single Subcutaneous Injection Silicone Breast Implant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Dr. Rose has served as expert witness on behalf of defendants in several legal proceedings involving silicone breast implants. He has also served as consultant for Mentor Corporation, a manufacturer of breast implants.Google Scholar
  2. 2.
    Vasey FB, Havice DL, Bocsnegra TS, et al (1994) Clinical and immunologic findings in fifty consecutive women with silicone breast implants and connective tissue disease. Semin Arthritis Rheum 24 (1 Suppl l):22–28PubMedCrossRefGoogle Scholar
  3. 3.
    Sahn EE, Garen PD, Silver, et al. (1990) Scleroderma following augmentation mammoplasty: report of case and review of the literature. Arch Dermatol 126:1198–1202PubMedCrossRefGoogle Scholar
  4. 4.
    Shons AR, Schubert W (1992) Silicone breast implants and immune disease. Ann Plast Surg 28:491–499PubMedCrossRefGoogle Scholar
  5. 5.
    Miyoshi K, Miyaoki T, Kobayashi Y, et al. (1962) Hypergammaglobulinemia by prolonged adjuvanticity in man: disorders developed after augmentation mammoplasty. Jap Med J: 2122, 9–14Google Scholar
  6. 6.
    Miyoshi K, Miyaoki T, Kobayashi Y (1973) Adjuvant disease in man. Clin Immunol 5:785–794Google Scholar
  7. 7.
    Pearson CM (1956) Development of arthritis, periarthritis, and periostitis in rats given adjuvants. Proc Soc Exp Med 91:95–100Google Scholar
  8. 8.
    Pearson CM, Wood FD (1981) Studies of polyarthritis and other lesions induced in rats by injection of mycobacterial adjuvant. I. General clinical and pathological characteristics and some modifying factors. Arthritis Rheum 2:440–459CrossRefGoogle Scholar
  9. 9.
    Nairn JO, Lanzafame, van Oss CJ (1993) The adjuvant effect of silicone gel on antibody formation in rats. Immunol Invest 22: 151CrossRefGoogle Scholar
  10. 10.
    Wilkerson P (1992) Report presented before the General and Plastic Surgery Device Evaluation Panel, FDA, Bethesda, MD, February 1992Google Scholar
  11. 11.
    Roitt IM (1994) Essential immunology. Blackwell Scientific Publications, BostonGoogle Scholar
  12. 12.
    Le Vier RR. Discussion of: Semple JL, Narini PP, Hay JB (193) Preparation techniques for silicone gel in the evaluation of its in vivo effects. Plast Reconstr Surg 92(5):979–81Google Scholar
  13. 13.
    Rose NR, Freidman H, Fahey JL, Eds (1986) Manual of clinical laboratory immunology, 3rd Ed. American Society for Microbiology, Washington, D.C.Google Scholar
  14. 14.
    Ratkay LG, Zhang L, Tonzetich J, Waterfield JD (1993) Complete Freund’s adjuvantinduces an earlier and more severe arthritis in MRL-lpr mice. Journal of Immunol 151(9):5081–7Google Scholar
  15. 15.
    Lopez-Guerrero JA, Lopez-Bote JP, Ortiz MA, et al (1993) Modulation of adjuvant arthritis in Lewis rats by recombinant vaccinia virus expressing the human 60-kiladalton heat shock protein. Infect Immun 61(10):4225–31PubMedGoogle Scholar
  16. 16.
    van Eden W (1991) Heat-shock proteins as immunogenic bacterial antigens with the potential to induce and regulate autoimmune arthritis. Immunol Rev 121:5–28PubMedCrossRefGoogle Scholar
  17. 17.
    Koga T, Pearson CM, Narita T, Kotani S (1973) Polyarthrities induced in the rats with cell walls from several bacteria and two streptomyces species. Proc Soc Exp Biol Med 143: 824PubMedGoogle Scholar
  18. 18.
    Whitehouse MW, Orr KJ, Beck FWJ, Pearson CM (1974) Freund’s adjuvants: relationship of arthritogenicity and adjuvanticity in rats to vehicle composition. Immunol 27:311Google Scholar
  19. 19.
    Kleinau S, Erlandsson H, Klareskog L (1994) Percutaneous exposure of adjuvant oil causes arthritis in DA rats. Clin Exp Immunol 96:281–284.PubMedCrossRefGoogle Scholar
  20. 20.
    Perretti M, Mudridge KG, Becherucci C, Parente L (1991) Evidence that interleukin-1 and lipoxygenase metabolites mediate the lethal effect of complete Freund’s adjuvant in adrenalectomized rats. Lymphokine Cytokine Res 10(4): 239–43PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • J. J. NicholsonIII
    • 1
  • G. E. Wong
    • 2
  • C. G. Frondoza
    • 1
    • 3
  • N. R. Rose
    • 1
    • 4
  1. 1.Department of Molecular Microbiology and ImmunologyJohns Hopkins UniversityUSA
  2. 2.Department of Molecular Microbiology and BiologyJohns Hopkins UniversityUSA
  3. 3.Department of Molecular Microbiology and Orthopaedic SurgeryJohns Hopkins UniversityUSA
  4. 4.Department of Molecular Microbiology and PathologyJohns Hopkins UniversityUSA

Personalised recommendations