Tissue Adhesives in Experimental Intestinal Anastomoses
The aim of this study was to investigate the effects of a synthetic and a biological glue in intestinal anastomoses healing processes. An experimental model was also adopted for evaluating the possible side effects linked to fibrin glue use which are reported in different studies on colonic anastomoses. Twenty male New Zealand white rabbits (b. w. 3 ± 0.350 kg) under general anaesthesia were used. Animals were divided into three groups of six rabbits each and in two animals sham operation was performed. In 18 animals 5 cm of the left colon was resected by midline laparotomy. The anastomoses were performed using three different techniques: group I, single-layer end-to-end anastomosis with interrupted 6-0 polyglycolic acid stitches, group II: four 6-0 polyglycolic acid stitches at the cardinal points plus butyl-2-cyanoacrylate; and in group III, four 6-0 polyglycolic acid stitches at the cardinal points plus fibrin glue. Animals were killed under general anaesthesia at days 7,14 and 21 for macroscopic and histologic analysis.
No intra- or post-operative complications were observed. In group I, three cases of perianastomotic granuloma were observed, and in two animals modest adhesion formation was found. In group II, adhesion formation was evident and, moreover, one animal at day 7 had an anastomotic leakage. In the same group, two cases of a macroscopically evident stenosis of the anastomosis were observed at day 14. In group III, no macroscopical complications were noticed. With histologie study the anastomoses that had healed best were the ones in groups I and III, while in group II superficial necrosis of the perivisceral adipose tissue occurred.
The results show the possibility of performing intestinal anastomoses with glues at least in experimental conditions. Moreover, anastomoses performed with biological glue seem to be more reliable and have less side effects.
KeywordsFibrin Glue Suture Material Zealand White Rabbit Fibrin Sealant Tissue Adhesive
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- 1.Arciola CR, Buscaroli S, Rocca M, Farinetti A, Fini M, Manfrini M, Giardino R (1990) Studio in vitro sull’adesività dello stafilococco epidermidis sui fili da sutura. Chir XI(3): 141–143Google Scholar
- 2.Buscaroli S, Arciola CR, Mancini I, Farinetti A, Fini M, Rocca M, Giardino R (1990) Influenza dei materiali da sutura sulla vitalità cellulare: studio comparativo sperimentale. G Chir XI(3): 147–148Google Scholar
- 3.Buscaroli S, Ciapetti G, Fini M, Rocca M, Giardino R (1990) T Lymphocytes activation and soluble receptor interleukin 2 release by biomedical polymers. Int J Artif Organs 13(9): 594Google Scholar
- 4.Buscaroli S, Stea S, Arciola CR, Rocca M, Nicoli Aldini N, Fini M, Mancini M, Giardino R (1990) II burst ossidativo per la determinazione della risposta immunitaria ai materiali di sintesi. G Chir XI(3): 144–146Google Scholar
- 5.Giardino R, Brulatti M, Franchini A (1986) Colonic anastomoses protected with fibrin sealant. In: Schlag G, Redl H. General surgery and abdominal surgery. Springer, Berlin Heidelberg New York, pp 155–158 (Fibrin sealant in operative medicine, vol 6)Google Scholar
- 6.Giardino R, Ussia G, Grilli Cicilioni C, Salerno A, Feldmann D, Farruggia F, Galletti G (1986) Colo-rectal anastomoses with fibrin sealant and transmural approaching device in pig. Eur Surg Res 18 [Suppl 1/86]Google Scholar
- 7.Giardino R (1987) La profilassi antibiotica in chirurgia. Bazzi, MilanoGoogle Scholar
- 8.Giardino R, Rocca M, Fini M, Buscaroli S, Arciola CR, Nicoli Aldini N, Morrone G (1992) Adesività batterica e reattività tessutale dei materiali per la sintesi: studio sperimentale in vitro ed in vivo. G. Chir XIII(4): 248–249Google Scholar