Abstract
The reorganization of the microtubular cytoskeleton was studied during telophase-interphase transition and interphase in Haemanthus endosperm cells, and in cell fragments (cytoplasts). This report concerns the role of microtubule (MT) converging centers (MTCCs) in the reorganization of the higher plant cytoskeleton. Microtubules (MTs) were visualized with the immunogold and immunogold-silver enhanced methods. Cells were fixed at room temperature (21°–24° C) and after high (35°–37° C) and low (4°–7° C) temperature shocks. The temperature shocks modify behavior of MTCCs. During early prophase and telophase-interphase transition, the formation of MTCCs is greatly enhanced at elevated temperature. These are stages when a pronounced reorganization of the cytoskeleton takes place. MTCCs are polar structures with remarkably different dynamics and properties at the diverging and converging ends. The indirect evidence shows that the converging tip of MTCC is (-) and the diverging end is (+). Our data imply that the reorganization of the higher plant cytoskeleton is basically a competitive sorting of MTs intrinsic polarity, with MTCCs as principal structural components.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Moscow State University, Biology Faculty, Department of Cytology and Histology, 119899 Moscow, Russia.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Baas PW and Ahmad FJ (1992) The plus ends of stable microtubules are the exclusive nucleating structures for microtubule in the axon. J. Cell Biol. 116:1231–1241
Bajer A (1965) Cine micrographic analysis of cell plate formation in endosperm. Exptl. Cell Res. 37:376–398
Bajer A (1968) Fine structure studies on phragmoplast and cell plate formation. Chromosoma 24:383–417
Bajer AS and Molè-Bajer J (1986) Reorganization of microtubules in endosperm cells and cell fragments of the higher plant Haemanthus in vivo. J. Cell Biol. 102:263–281
Bornens M (1992) Structure and function of isolated centrosomes. In The Centrosome (ed. VI Kalnins) pp. 1–43. Acad Press San Diego
Brinkley BR (1985) Microtubule organizing centers. Ann. Rev. Cell Biol. 1:145–172
Cassimeris L Inoué S and Salmon ED (1988) Microtubule dynamics in the chromosomal spindle fiber: analysis by fluorescence and high-resolution polarization microscopy. Cell Mot. Cytoskel. 10:185–196
Euteneuer U and Mcintosh JR (1980) Polarity of midbody and phragmoplast microtubules. J. Cell Biol. 87:509–515
Euteneuer U Jackson W T and Mcintosh JR (1982) Polarity of spindle microtubules in Haemanthus endosperm. J. Cell Biol. 94, 644–653
Farell KW Jordan MA Miller HP and Wilson L (1987) Phase dynamics at MT ends: the coexistence of dynamic instability and treadmilling. J. Cell Biol. 104:1035–1046
Gelfand V I and Bershadsky AD (1991) Microtubule dynamics: mechanism, regulation, and function. Annu. Rev. Cell Biol. 7:93–116
Gunning BES and Hardham AR (1982) Microtubules. Ann. Rev. Plant Physiol. 33:651–698
Harris PJ Clason EL and Prier KR (1989) Tubulin polymerization in unfertilized sea-urchin eggs induced by elevated temperature. J. Cell. Sci. 93:9–17
Harris PJ and Clason EL (1992) Conditions for assembly of tubulin-based structures in unfertilized sea urchin eggs. Spirals, monasters and cytasters. J. Cell Sci. 102:557–567
Hepler PK and Jackson WT (1967) Microtubules and early stages of cell-plate formation in the endosperm of Haemanthus katerinae Baker. J. Cell Biol. 38:437–446
Kallajoki M Weber K and Osborn M (1992) Ability to organize microtubules in taxol treated mitotic PtK2 cells goes with the SPN antigen and not with centrosome. J. Cell Sci. 102:91–102
Maekawa T Leslie R and Kuriyama R (1991) Identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells. Eur. J. Cell Biol. 94:255–267
McDonald HB Stewart RJ and Goldstein LSB (1990) The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor. Cell 63:1159–1165
McNiven MA Wang M and Porter KR (1984) Microtubule polarity and the direction of pigment transport reverse simultaneously in surgically severed melanophore arms. Cell. 37:753–765
McNiven MA and Portēr KR (1988) Organization of microtubules in centrosome free cytoplasm. J. Cell Biol 106:1593–1605
Mineyuki Y Yamashita M and Nagahama Y (1991) p34cdc2 kinase homologue in the preprophase band. Protoplasma 162:182–186
Mitchison TJ (1992) Self-organization of polymer-motor systems in the cytoskeleton. Phil. Trans. R. Soc. Lond. B. 336:99–106
Molè-Bajer J and Bajer AS (1983) The action of taxol on mitosis. Modification of microtubule arrangements of the mitotic spindle. J. Cell Biol. 96:527–540
Molè-Bajer J and Bajer AS (1988) Relation of F-actin organization to microtubules in drug treated Haemanthus mitosis. Protoplasma Suppl. 1:99–112
Morejohn LC and Fosket DE (1991) The biochemistry of compounds with anti-microtubule activity in plant cells. Pharmac. Theor. 51:217–231.
Rieder CL and Bajer AS (1977) Heat-induced reversible hexagonal packing of spindle microtubule. J. Cell Biol. 74:717–725
Sinnott HB and Bloch R (1941) Division in vacuolate plant cells. Am. J. Bot. 28:225–232
Smirnova EA and Bajer AS (1992a) Spindle poles in higher plant mitosis. Cell Mot. Cytosk. 23:1–7
Smirnova EA and Bajer AS (1992b) Microtubule converging centers and reorganization of the mitotic spindle in higher plant Haemanthus. (Submitted).
Schulze E and Kirschner M (1986) Microtubule dynamics in interphase cells. J.Cell Biol. 102:1020–1031.
Vantard M Levilliers N Hill A-M Adoutte A and Lambert A-M (1990) Incorporation of Paramecium axonemal tubulin into higher plant cells reveals functional sites of microtubule assembly. Proc. Natl. Acad. Sci. USA. 87:8825–8829.
Verde F Labbe J-C Dorre M and Karsenti E (1990) Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs. Nature 343:233–238.
Verde F Berrez J-M Antony C and Karsenti E (1991) Taxol induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein. J. Cell Biol. 112:1177–1187.
Verde F Dogterom M Stelzer E Karsenti E and Leibler S (1992) Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus extracts. J. Cell Biol. 118:10974109.
Vorobjev IA and Nadezhdina ES (1987) The centrosome and its role in the organization of microtubules. Int. Rev. Cytol. 106:227–293
Wadsworth P and Salmon ED (1986) Microtubule dynamics of mitotic spindles of living cells. Ann. N. Y. Acad. Sci. 466:580–592
Wick SM and Duniec J (1984) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. II. Transition between pre-prophase band and the mitotic spindle. Protoplasma 122:45–55
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Bajer, A.S., Smirnova, E.A., Molè-Bajer, J. (1993). Microtubule Converging Centers — Implications for Microtubule Dynamics in Higher Plants. In: Vig, B.K. (eds) Chromosome Segregation and Aneuploidy. NATO ASI Series, vol 72. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84938-1_19
Download citation
DOI: https://doi.org/10.1007/978-3-642-84938-1_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-84940-4
Online ISBN: 978-3-642-84938-1
eBook Packages: Springer Book Archive