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Mutations Affecting Translational Accuracy in Yeast

  • Conference paper
Protein Synthesis and Targeting in Yeast

Part of the book series: NATO ASI Series ((ASIH,volume 71))

Abstract

To investigate the mechanisms used in eukaryotes to maintain translational accuracy, we and others have isolated and characterized mutations that affect the fidelity of protein synthesis in yeast. These mutations include nonsense suppressors that cause misreading of specific stop codons; omnipotent suppressors, which cause general translational inaccuracy and suppression of UAG, UAA, and UGA codons; and allosuppressors and antisuppressors, which respectively enhance or lower the efficiency of certain suppressors. Using this layered approach we should identify translational components that are likely to interact. Recently the genetic work has been extended by cloning and characterizing the mutant genes. We have also identified components of the translational apparatus important for fidelity by selecting for wild-type genes that alter accuracy when present at higher copy number. Below we summarize our work in this area.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences Laboratory for Molecular Biology, University of Illinois at Chicago, Chicago, Illinois, 60680, USA

    Susan W. Liebman, Annette Vincent, Jae Mahn Song & Jamie All-Robyn

Authors
  1. Susan W. Liebman
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  2. Annette Vincent
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  3. Jae Mahn Song
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  4. Jamie All-Robyn
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Editor information

Editors and Affiliations

  1. Department of Molecular & Cell Biology, University of Aberdeen, Marischal College, Aberdeen, AB9 1AS, UK

    Alistair J. P. Brown

  2. Biological Laboratory, The University, Canterbury, Kent, CT2 7NJ, UK

    Mick F. Tuite

  3. Department of Gene Expression, GBF, Mascheroder Weg 1, 3300, Braunschweig, Germany

    John E. G. McCarthy

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© 1993 Springer-Verlag Berlin Heidelberg

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Liebman, S.W., Vincent, A., Song, J.M., All-Robyn, J. (1993). Mutations Affecting Translational Accuracy in Yeast. In: Brown, A.J.P., Tuite, M.F., McCarthy, J.E.G. (eds) Protein Synthesis and Targeting in Yeast. NATO ASI Series, vol 71. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84921-3_9

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  • DOI: https://doi.org/10.1007/978-3-642-84921-3_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84923-7

  • Online ISBN: 978-3-642-84921-3

  • eBook Packages: Springer Book Archive

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