Approaches Towards a Genetic Analysis of the Nuclear Pore Complex in Yeast

  • Christian Wimmer
  • Valérie Doye
  • Ulf Nehrbass
  • Nikolaus Schlaich
  • Eduard C. Hurt
Part of the NATO ASI Series book series (volume 71)


The nuclear pore complex (NPC) mediates the nucleocytoplasmic transport through the nuclear envelope. It is a supramolecular, cylindrical structure with an overall diameter of ~130 nm, a thickness ~ 70 nm (Akey, 1989) and a molecular mass of 125 MDa (Reichelt et al. 1990). The pore consists of two concentric rings on the inner and outer face of the nuclear membrane, eight radial spokes and a central plug (Unwin and Milligan, 1982; Reichelt et al. 1990; Jarnik and Aebi, 1991). Recent EM studies revealed, besides a central channel, eight smaller peripheral channels with a diameter of 10 nm which could be used for diffusion of small molecules (Hinshaw et al. 1992). The NPC is associated with cytoskeletal structures: a basketlike network at the nucleoplasmic side and fibrillar elements directing into the cytoplasm (Akey, 1989; Reichelt et al. 1990).


Nuclear Pore Complex Nuclear Periphery Nuclear Localization Sequence Synthetic Lethality Nucleocytoplasmic Transport 
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  1. Akey CW (1989) Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy. J Cell Biol 109: 955–970PubMedCrossRefGoogle Scholar
  2. Bender A, Pringle JR (1991) Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae. Mol Cell Biol 11: 1295–1305PubMedGoogle Scholar
  3. Botstein D, Fink GR (1988) Yeast: an experimental organism for modern biology. Science 240: 1439–1443PubMedCrossRefGoogle Scholar
  4. Carmo-Fonseca M, Hurt EC (1991) Across the nuclear pores with the help of nucleoporins. Chromosoma 101: 199–205PubMedCrossRefGoogle Scholar
  5. Carmo-Fonseca M, Kern H, Hurt EC (1991) Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur J Cell Biol 55: 17–30PubMedGoogle Scholar
  6. Costigan C, Gehrung S, Snyder M (1992) A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast cell morphogenesis and cell growth. Mol Cell Biol 12: 1162–1178PubMedGoogle Scholar
  7. Davis LI, Fink GR (1990) The NUP1 Gene Encodes an Essential Component of the Yeast Nuclear Pore Complex. Cell 61: 965–978PubMedCrossRefGoogle Scholar
  8. Dingwall C, Laskey RA (1991) Nuclear targeting sequences-A consensus? Trends Biochem Sci 16: 478–481PubMedCrossRefGoogle Scholar
  9. Dworetzky SI, Feldherr CM (1988) Translocation of RNA-coated gold particles through the nuclear pores of oocytes. J Cell Biol 06: 575–584CrossRefGoogle Scholar
  10. Finlay DR, Newmeyer DD, Price TM, Forbes DJ (1987) Inhibition of invitro nuclear transport by a lectin that binds to nuclear pores. J Cell Biol 104: 189–200PubMedCrossRefGoogle Scholar
  11. Goldfarb DS, Michaud N (1991) Pathways for the nuclear transport of proteins and RNAs. Trends Cell Biol 1: 20–24PubMedCrossRefGoogle Scholar
  12. Hinshaw JE, Carragher BO, Milligan RA (1992) Architecture and design of the nuclear pore complex. Cell 69: 1133–1141PubMedCrossRefGoogle Scholar
  13. Huffaker TC, Hoyt MA, Botstein D (1987) Genetic Analysis of the Yeast Cytoskeleton. Annu Rev Genet 21: 259–284PubMedCrossRefGoogle Scholar
  14. Hurt EC (1988) A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae. EMBO J 7: 4323–4334PubMedGoogle Scholar
  15. Hurt EC (1989) NSP1, a yeast protein located at the nuclear periphery is required for the cell-cycleperiphery, is required for the cell cycle of Saccharomyces cerevisiae. J Cell Sci 12: 243–252Google Scholar
  16. Hurt EC (1990) Targeting of a cytosolic protein to the nuclear periphery. J Cell Biol 111: 2829–2837PubMedCrossRefGoogle Scholar
  17. Hurt EC, Mutvei A, Carmo-Fonseca M (1991) The nuclear envelope of the yeast Saccharomyces cerevisiae. Int Rev Cytol in pressGoogle Scholar
  18. Jarnik M, Aebi U (1991) Toward a more complete 3-D structure of the nuclear pore complex. J Struct Biol 107: 291–308PubMedCrossRefGoogle Scholar
  19. Kalderon D, Roberts BL, Richardson WP, Smith AE (1984) A short amino acid sequence able to specify nuclear location. Cell 39: 499–509PubMedCrossRefGoogle Scholar
  20. Koshland D, Kent JC, Hartwell LH (1985) Genetic analysis of the mitotic transmission of minichromosomes. Cell 40: 393–403PubMedCrossRefGoogle Scholar
  21. Kranz JE, Holm C (1990) Cloning by function: an alternative approach for identifying yeast homologs of genes from other organisms. Proc Natl Acad Sci USA 87: 6629–6633PubMedCrossRefGoogle Scholar
  22. Michaud N, Goldfarb DS (1991) multiple pathways in nuclear transport: The import of U2 snRNP occurs by a novel kinetic pathway. J Cell Biol 112: 215–223PubMedCrossRefGoogle Scholar
  23. Moore MS, Blobel G (1992) The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors. Cell 69: 939–950PubMedCrossRefGoogle Scholar
  24. Nehrbass U, Kern H, Mutvei A, Horstmann H, Marshallsay B, Hurt EC (1990) NSP1: A Yeast Nuclear Envelope Protein Localized at the Nuclear Pores Exerts its Essential Function by its Carboxyterminal Domain. Cell 61: 979–989PubMedCrossRefGoogle Scholar
  25. Newmeyer DD, Forbes DJ (1988) Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation. Cell 52: 641–653PubMedCrossRefGoogle Scholar
  26. Reichelt R, Holzenburg EL, Buhle EL, Jarnik M, Engel A, Aebi U (1990) Correlation between structure and mass distribution of nuclear pore complex components. J Cell Biol 110: 883–894PubMedCrossRefGoogle Scholar
  27. Richardson WD, Mills AD, Dilworth SM, Laskey RA, Dingwall C (1988) Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores. Cell 52: 655–664PubMedCrossRefGoogle Scholar
  28. Robbins J, Dilworth SM, Laskey RA, Dingwall C (1991) Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: Identification of a class of bipartite nuclear targeting sequence. Cell 64: 615–623PubMedCrossRefGoogle Scholar
  29. Snow CM, Senior A, Gerace L (1987) Monoclonal antibodies identify a group of nuclear pore complex glycoproteins. J Cell Biol 104: 1143–1156PubMedCrossRefGoogle Scholar
  30. Unwin PN, Milligan RA (1982) A large particle associated with the perimeter of the nuclear pore complex. J Cell Biol 93: 63–75PubMedCrossRefGoogle Scholar
  31. Wertman KF, Dublin DG and Botstein D (1991) Mutational analysis of the actin gene. Abstract book of CSH Meeting ‘Yeast cell biology’, pp 3Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Christian Wimmer
    • 1
  • Valérie Doye
    • 1
  • Ulf Nehrbass
    • 1
  • Nikolaus Schlaich
    • 1
  • Eduard C. Hurt
    • 1
  1. 1.EMBLHeidelbergF. R. Germany

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