Abstract
Research over the past ten years has yielded information about the mechanism and some of the factors required for the specific uptake of certain proteins by the nucleus (see, for example, Garcia-Bustos et al., 1991; Silver, 1991; Stochaj and Silver, 1992 for recent review). Proteins move from their site of synthesis in the cytoplasm to the nuclear envelope. Specific targeting to the nucleus is accomplished in some cases by the presence of a short stretch of amino acids on the nuclear-destined proteins, termed an NLS for nuclear localization sequence. The NLS may mediate the interaction of the transported protein with cytoplasmic carriers or “receptors.” We know that proteins enter the nucleus via an aqueous channel formed by a complex of proteins in the nuclear envelope (termed the NPC for nuclear pore complex [Feldherr et al., 1984]). NLS-containing proteins may pass through the pore in association with the receptor or released from the receptor at the pore entry. The import process has been shown to require ATP in animal cells, Xenopus oocytes and yeast (Newmeyer and Forbes, 1988; Richardson et al., 1988; Breeuwer and Goldfarb, 1990; Garcia-Bustos et al., 1991).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bossie, M., DeHoratius, C., Barcelo, G., and Silver, P. (1992). A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast. Molecular Biology of the Cell, In press.
Breeuwer, M. and Goldfarb, D. (1990). Facilitated nuclear transport of histone H1 and other small nucleophilic proteins. Cell 60: 999–1008.
Davis, L. and Fink, G. R. (1990). The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61: 965–978.
Dingwall, C., Sharnick, S.V., and Laskey, R.A. (1982). A polypeptide domain that specifies migration of nucleoplasmin into the nucleus. Cell 30: 449–458.
Dingwall, C. and Laskey, R.A. (1991). Nuclear targeting sequences-a consensus? Trends in Biochem. sci. 16: 478–481.
Feldherr, C., Kallenbach, E., and Schultz, N. (1984). Movement of a karyophillic protein through the nuclear pores of oocytes. J. Cell Biol. 99: 2216–2222.
Garcia-Bustos, J., Heitman, J., and Hall, M. (1991). Nuclear protein localization. Biochim. Biophys. Acta. 1071: 83–101.
Goldfarb, D.S., Gariepy, J., Schoolnik, G. and Kornberg, R. (1986). Synthetic peptides as nuclear localization signals. Nature 322: 641–644.
Kalderon, D., Roberts, B.L., Richardson, W.D., and Smith, A.E. (1984). A short amino acid sequence able to specify nuclear location. Cell 39: 499–509.
Lanford, R.E. and Butel, J.S. (1984). Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell 37: 801–813.
Lee, W.-C, Xue, Z., and Melese, T. (1991). The NSR1 gene encodes a protein that specifically binds nuclear localization sequences and has two RNA recognition motifs. J. Cell Biol. 113: 1–12.
Meier, U.T. and Blobel, G. (1990). A nuclear localization signal binding protein in the nucleolus. J. Cell Biol. 111: 2235–2245.
Nelson, M. and Silver, P. (1989). Context affects nuclear protein localization in Saccharomyces cerevisiae. Mol. Cell. Biol. 9: 384–389.
Newmeyer, D.D. and Forbes, D. (1988). Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation. Cell 52: 641–653.
Richardson, W.D., Mills, A.D., Dilworth, S.M., Laskey, R.A. and Dingwall, C. (1988). Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores. Cell 52: 655–664.
Rothblatt, J.A., Deshaies, R., Sanders, S., Dawn, G. and Schekman, R. (1989). Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J. Cell Biol. 109: 2641–2652.
Sadler, I., Chiang, A., Kurihara, T., Rothblatt, J., Way, J. and Silver, P. (1989). A yeast gene important for assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an E. coli heat shock protein. J. Cell Biol. 109: 2665–2675.
Silver, P., Keegan, L., and Ptashne, M. (1984). Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization. Proc. Natl. Acad. sci. 81: 5951–5955.
Silver, P., Chiang, A. and Sadler, I. (1988). Mutations that alter both localization and production of a yeast nuclear protein. Genes Dev. 2: 707–717.
Silver, P., Sadler, I. and Osborne, M. (1989). Yeast proteins that recognize nuclear localization sequences. J. Cell Biol. 109: 983–989.
Silver, P. (1991). How proteins enter the nucleus. Cell 64: 489–497.
Stochaj, U. and Silver, P. (1992). Nucleocytoplasmic traffic of proteins. Eur. J. of Cell Biol., In press.
Stochaj, U., Osborne, M., Kurihara, T., and Silver, P. (1991). A yeast protein that binds nuclear localization signals: Purification, localization, and antibody inhibition of activity. J. Cell Biol. 113: 1243–1254.
Stochaj, U. and Silver, P. (1992). A conserved phosphoprotein that specifically binds nuclear localization sequences is involved in nuclear import. J. Cell Biol. 117: 473–482.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Silver, P. (1993). Using Yeast to Study Exchange of Macromolecules between the Cytoplasm and the Nucleus. In: Brown, A.J.P., Tuite, M.F., McCarthy, J.E.G. (eds) Protein Synthesis and Targeting in Yeast. NATO ASI Series, vol 71. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84921-3_23
Download citation
DOI: https://doi.org/10.1007/978-3-642-84921-3_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-84923-7
Online ISBN: 978-3-642-84921-3
eBook Packages: Springer Book Archive