Yeast Mitochondrial Translation: Nuclear Genes Involved in the Expression of the Mitochondrial Genome

  • T. Rinaldi
  • M. Valens
  • M. Bolotin-Fukuhara
Conference paper
Part of the NATO ASI Series book series (volume 71)


A search for nuclear genes involved in mitochondrial translation has been made using genetic suppressors and multicopy suppressors of mitochondrial mutations which affect protein synthesis.

Three different mitochondrial mutations ( two localized in the 21SrRNA gene and one in the Asp tRNA gene) have been chosen for this purpose. In all cases, nuclear suppressors have been characterized which have biochemical and physiological properties compatible with components of the translational apparatus. Cloning one of those led us to the identification of a new yeast gene called SMT1.

Several multicopy suppressors have also been isolated. With this method, we uncovered a new pathway related to mitochondrial translation.We also found that overexpression of both mtEF-Tu and mtAspartyl tRNA synthetase can correct a deficiency in the 3′ end maturation of the mitochondrial Asp tRNA.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • T. Rinaldi
    • 1
  • M. Valens
    • 1
  • M. Bolotin-Fukuhara
    • 1
  1. 1.Laboratoire de Génétique Moléculaire. I G M ( URA D 1354 du CNRS)Université Paris-Sud.Orsay CedexFrance

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