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Yeast Mitochondrial Translation: Nuclear Genes Involved in the Expression of the Mitochondrial Genome

  • Conference paper
Protein Synthesis and Targeting in Yeast

Part of the book series: NATO ASI Series ((ASIH,volume 71))

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Abstract

A search for nuclear genes involved in mitochondrial translation has been made using genetic suppressors and multicopy suppressors of mitochondrial mutations which affect protein synthesis.

Three different mitochondrial mutations ( two localized in the 21SrRNA gene and one in the Asp tRNA gene) have been chosen for this purpose. In all cases, nuclear suppressors have been characterized which have biochemical and physiological properties compatible with components of the translational apparatus. Cloning one of those led us to the identification of a new yeast gene called SMT1.

Several multicopy suppressors have also been isolated. With this method, we uncovered a new pathway related to mitochondrial translation.We also found that overexpression of both mtEF-Tu and mtAspartyl tRNA synthetase can correct a deficiency in the 3′ end maturation of the mitochondrial Asp tRNA.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire. I G M ( URA D 1354 du CNRS), Université Paris-Sud., 91405, Orsay Cedex, France

    T. Rinaldi, M. Valens & M. Bolotin-Fukuhara

Authors
  1. T. Rinaldi
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  2. M. Valens
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  3. M. Bolotin-Fukuhara
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Editor information

Editors and Affiliations

  1. Department of Molecular & Cell Biology, University of Aberdeen, Marischal College, Aberdeen, AB9 1AS, UK

    Alistair J. P. Brown

  2. Biological Laboratory, The University, Canterbury, Kent, CT2 7NJ, UK

    Mick F. Tuite

  3. Department of Gene Expression, GBF, Mascheroder Weg 1, 3300, Braunschweig, Germany

    John E. G. McCarthy

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© 1993 Springer-Verlag Berlin Heidelberg

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Rinaldi, T., Valens, M., Bolotin-Fukuhara, M. (1993). Yeast Mitochondrial Translation: Nuclear Genes Involved in the Expression of the Mitochondrial Genome. In: Brown, A.J.P., Tuite, M.F., McCarthy, J.E.G. (eds) Protein Synthesis and Targeting in Yeast. NATO ASI Series, vol 71. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84921-3_17

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  • DOI: https://doi.org/10.1007/978-3-642-84921-3_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84923-7

  • Online ISBN: 978-3-642-84921-3

  • eBook Packages: Springer Book Archive

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