Yeast Mitochondrial Translation: Nuclear Genes Involved in the Expression of the Mitochondrial Genome
A search for nuclear genes involved in mitochondrial translation has been made using genetic suppressors and multicopy suppressors of mitochondrial mutations which affect protein synthesis.
Three different mitochondrial mutations ( two localized in the 21SrRNA gene and one in the Asp tRNA gene) have been chosen for this purpose. In all cases, nuclear suppressors have been characterized which have biochemical and physiological properties compatible with components of the translational apparatus. Cloning one of those led us to the identification of a new yeast gene called SMT1.
Several multicopy suppressors have also been isolated. With this method, we uncovered a new pathway related to mitochondrial translation.We also found that overexpression of both mtEF-Tu and mtAspartyl tRNA synthetase can correct a deficiency in the 3′ end maturation of the mitochondrial Asp tRNA.
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- Berensten SM, Jahn M, Söll D ( 1992) Aminoacyl tRNA synthetase-induced cleavage of tRNA. Nucl. Acids Res. 20: 1523–1530Google Scholar
- Bolotin-Fukuhara M, Sor F, Fukuhara H (1983) Mitochondrial ribosomal RNA mutations and their nuclear suppressors. In: “Mitochondria 83 ”. Schweyen H et al, eds. Walter de Gruyter, Berlin, pp. 455–467Google Scholar
- Daignan-Fornier B, Su I, Contamine V, Bolotin-Fukuhara M (1988) A genetic approach of molecular interactions in yeast mitochondrial ribosomes. In “Genetics of Translation: New Approaches” M. Tuite, M. Picard and M. Bolotin-Fukuhara, eds. NATO/ASU Workshop. Springer-Verlag, Berlin, pp. 159–169Google Scholar
- Daignan-Fornier B, Bolotin-Fukuhara M (1989) Functional exploration of the yeast (-S. cerevisiae ) genome: use of a mini-Mu transposon to analyse randomly cloned DNA sequences. Yeast, 5: 259–269.Google Scholar
- Faye G, Bolotin-Fukuhara M, Fukuhara H (1976) Mitochondrial mutations that affect mitochondrial transfer ribonucleic acid in Saccharomyces cerevisiae. In: “ The Genetics and Biogenesis of Chloroplasts and Mitochondria”. Ed. T. Bücher et al. Elsevier/North Holland Biomedical Press. Amsterdam. The Netherlands, pp: 613–618Google Scholar
- Groudinski O, Dujardin G, Slonimski PP (1981) Long range control circuits within mitochondria and between nucleus and mitochondria. II. Genetic and biochemical analyses of suppressors which selectively alleviate the mitochondrial intron mutations. Mol. Gen. Genet. 184: 493–503Google Scholar
- Guetari M, Zelikson R, Bolotin-Fukuhara M (1987) Nucleo-mitochondrial interactions involved in the biogenesis of mitochondrial ribosomes: identification of a possible conditional mutation of the Var 1 gene and its nuclear suppressors. Mol. Gen. (Life sci. Adv. ) 6: 29–34Google Scholar