Aberrant mRNA Decoding in the Dimorphic Yeast Candida Albicans
The role tRNAs play in protein synthesis has been the subject of considerable scientific effort and debate during the last 30 years. From the early studies, the consensus view that tRNAs are primarily responsible for decoding genetic information was established. It was then assumed that tRNAs decoded only the codons allowed by correct Watson-Crick base pairing between the codon and the anticodon. Later developments allowed the formulation of the “wobble” hypothesis, to explain non-Watson-Crick base pairing at the first anticodon position (Crick, 1966). This concept of decoding perpetuated the idea that an anticodon, with a certain degree of freedom at the first base, was the only structural determinant of decoding. However, recent studies point to the fine detail of tRNA tertiary structure as also being an important determinant of accurate and efficient decoding, as is well illustrated by misreading and frameshifting tRNAs whose phenotypes can be determined by specific alterations in their primary structures outside the anticodon (Hatfield et al, 1990; Atkins et al, 1991; reviews).
KeywordsRabbit Reticulocyte Lysate Brome Mosaic Virus Yeast Candida ALBICANS Cloverleaf Structure Suppressor tRNA
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