Summary
Using the transgenic mouse approach, we investigated the mechanisms that regulate expression of the human neurofilament light (NF-L) and heavy (NF-H) genes. Both human NF-L and NF-H genes are expressed in a tissue-specific fashion in transgenic mice. For the NF-L gene, various constructs have been tested in transgenic mice to define regulatory regions contributing to neuronal expression. Unexpectedly, this analysis demonstrated the presence of intragenic elements in the NF-L gene that confer cell-type specific regulation on the basal activity of the proximal NF-L promoter. Moreover, the examination of transgenic mice bearing a high-copy number of the NF-L gene revealed the existence of a translational control impeding overexpression of NF-L in CNS neurons. Unlike the NF-L gene, overexpression of the human NF-H gene in transgenic mice was not impeded by the existence of such posttranscriptional controls.
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© 1992 Springer-Verlag Berlin Heidelberg
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Charron, G., Beaudet, L., Côté, E., Houle, D., Julien, JP. (1992). Regulation of the Human Neurofilament Light and Heavy Genes in Transgenic Mice. In: Gage, F.H., Christen, Y. (eds) Gene Transfer and Therapy in the Nervous System. Research and Perspectives in Neurosciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84842-1_16
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DOI: https://doi.org/10.1007/978-3-642-84842-1_16
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