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Gene Transfer and Therapy in the Nervous System pp 133–145Cite as

Development of Herpes Simplex Virus as a Gene Transfer Vector for the Central Nervous System

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Part of the book series: Research and Perspectives in Neurosciences ((NEUROSCIENCE))

Abstract

Herpes simplex virus (HSV) is a large neurotropic virus containing 152 kb of linear dsDNA encoding at least 70 gene products. In natural human infections, the virus replicates primarily in skin or mucosal epithelial cells prior to entering regional axon terminals and retrograde transport of the virus capsid to sensory ganglia, where it may either replicate or establish latency (Roizman and Sears 1990; Fig. 1). Latent virus can be reactivated by a variety of stimuli followed by anterograde transport and productive secondary infection at or near the primary site. In animals models, infection of peripheral sensory ganglia and latency can be achieved through inoculation of the skin (Cook and Stevens 1973), cornea (Sciler and Schwab 1984) or the olfactory bulb after intranasal inoculation (Dobson et al. 1989). Focal infection of specific brain regions can be achieved by stereotactic injection of small volumes of virus into defined brain regions (McFarland et al. 1986; Bak et al. 1977).

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Authors
  1. J. C. Glorioso
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  2. L. R. Sternberg
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  3. W. F. Goins
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  4. D. J. Fink
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Editor information

Editors and Affiliations

  1. Department of Neurosciences, The University of California, San Diego, La Jolla, 92093-0624, USA

    Fred H. Gage

  2. Fondation IPSEN pour la Recherche Thérapeutique, 30, rue Cambronne, 75737, Paris Cedex 15, France

    Yves Christen

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© 1992 Springer-Verlag Berlin Heidelberg

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Glorioso, J.C., Sternberg, L.R., Goins, W.F., Fink, D.J. (1992). Development of Herpes Simplex Virus as a Gene Transfer Vector for the Central Nervous System. In: Gage, F.H., Christen, Y. (eds) Gene Transfer and Therapy in the Nervous System. Research and Perspectives in Neurosciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84842-1_11

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  • DOI: https://doi.org/10.1007/978-3-642-84842-1_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84844-5

  • Online ISBN: 978-3-642-84842-1

  • eBook Packages: Springer Book Archive

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