Picosecond Time-Resolved Resonance Raman Spectroscopy of Bacteriorhodopsin: Structure and Kinetics of the J, K, and KL Intermediates

  • S. J. Doig
  • P. J. Reid
  • R. A. Mathies
Part of the Springer Proceedings in Physics book series (SPPHY, volume 68)


Bacteriorhodopsin (BR) is an intrinsic membrane protein that functions as a light-driven proton pump [1]. Light absorption by its all-trans retinal protonated Schiff base prosthetic group initiates a trans → 13-cis isomerization. The first ground-state photoproduct, called J, forms in only 500 fs [2]. J decays to the K intermediate in 3 ps [3] which forms L in about 1 μs. The structural changes of the chromophore that accompany the J to K transition are not well defined. Also, although some studies have suggested the presence of an additional intermediate between K and L called “KL” [4, 5], this transition has not been characterized.


Probe Pulse Purple Membrane Resonance Raman Spectrum Protein Conformational Change Intrinsic Membrane Protein 
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  1. 1.
    R.A. Mathies, S.W. Lin, J.B. Ames, W.T. Pollard: Annu. Rev. Biophys. Biophys. Chem. 20, 491 (1991)CrossRefGoogle Scholar
  2. 2.
    R.A. Mathies, C.H. Brito Cruz, W.T. Pollard, CV. Shank: Science 240, 111 (1988)CrossRefGoogle Scholar
  3. 3.
    H.J. Polland, M.A. Franz, W. Zinth, W. Kaiser, E. Rolling, D. Oesterhelt: Biophys. J. 49, 651 (1986)CrossRefGoogle Scholar
  4. 4.
    S.J. Milder, D.S. Kliger: Biophys. J. 53, 465 (1988)CrossRefGoogle Scholar
  5. 5.
    Y. Shichida, S. Matuoka, Y. Hidaka, T. Yoshizawa: Biochim. Biophys. Acta 723, 240 (1983)CrossRefGoogle Scholar
  6. 6.
    C. Hsieh, M.A. El-Sayed, M. Nicol, M. Nagumo, J. Lee: Photochem. Photobiol. 38, 83 (1983)CrossRefGoogle Scholar
  7. 7.
    D. Stern, R. Mathies: In Time-Resolved Vibrational Spectroscopy, ed. M. Stockburger, A. Laubereau (Springer-Verlag, Berlin, New York 1985) Vol. 4, p. 250Google Scholar
  8. 8.
    G.H. Atkinson, T.L. Brack, D. Blanchard, G. Rumbles: Chem. Phys. 131, 1 (1989)CrossRefGoogle Scholar
  9. 9.
    R. van den Berg, D. Jang, H.C. Bitting, M.A. El-Sayed: Biophys. J. 58, 135 (1990)CrossRefGoogle Scholar
  10. 10.
    S.O. Smith, J.A. Pardoen, J. Lugtenburg, R.A. Mathies: J. Phys. Chem. 91, 804 (1987)CrossRefGoogle Scholar
  11. 11.
    S J. Doig, P.J. Reid, R.A. Mathies: J. Phys. Chem. (in press)Google Scholar
  12. 12.
    M. Braiman, R. A. Mathies: Proc. Natl. Acad. Sci. USA 79, 403 (1982)ADSCrossRefGoogle Scholar
  13. 13.
    S.P.A. Fodor, J.B. Ames, R. Gebhard, E.M.M. van den Berg, W. Stoeckenius, J. Lugtenburg, R.A. Mathies: Biochem. 27, 7097 (1988)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • S. J. Doig
    • 1
  • P. J. Reid
    • 1
  • R. A. Mathies
    • 1
  1. 1.Chemistry DepartmentUniversity of CaliforniaBerkeleyUSA

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