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Application of Polymerase Chain Reaction to Hepatitis C Virus Research and Diagnostics

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Diagnosis of Human Viruses by Polymerase Chain Reaction Technology

Part of the book series: Frontiers of Virology ((FRVIROLOGY,volume 1))

Summary

Rapid advances in virology during the 1980s were due, in part, to the development of powerful molecular biological technologies including lambda GT11 immunoscreening (Young and Davis, 1983) and the polymerase chain reaction (PCR) (Saiki et al. 1985). The hepatitis C virus (HCV), which was cloned using lambda GT11 immunoscreening, is the only virus which was discovered by cloning and characterizing the viral genome prior to biochemical isolation and/or visualization of the virus or viral antigens by microscopy. The application of PCR to HCV enabled researchers to overcome some of the technical limitations associated with the relatively low titers of HCV in blood or liver tissue and the absence of an in vitro tissue culture system for the cultivation of HCV. PCR based diagnostics, the rapid accumulation of HCV sequence information and the availability of cDNA/PCR generated cDNA clones for the expression of putative viral antigens has contributed greatly to our understanding of the structure and biology of HCV.

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  1. Chiron Corporation, 4560 Horton St., Emeryville, CA, 94608, USA

    Amy J. Weiner, Venkatakrishna Shyamala, J. Eric Hall, Michael Houghton & Jang Han

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  1. Amy J. Weiner
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  2. Venkatakrishna Shyamala
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  3. J. Eric Hall
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  4. Michael Houghton
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Editors and Affiliations

  1. Department of Molecular Virology Faculty of Medicine, Hebrew University of Jerusalem, POB 1172, Jerusalem, Israel

    Yechiel Becker

  2. Institut für Medizinische Virologie, der Universität Heidelberg, Im Neuenheimer Feld 324, W-6900, Heidelberg, Germany

    Gholamreza Darai

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Weiner, A.J., Shyamala, V., Hall, J.E., Houghton, M., Han, J. (1992). Application of Polymerase Chain Reaction to Hepatitis C Virus Research and Diagnostics. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_8

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