Summary
The results of two studies involving 54 children born to HIV-1 seropositive women are reported. In the first one, I tested 24 children by PCR and viral isolation from peripheral blood mononuclear cells: Results were concordant in 96% of the cases, showing that PCR is a valid alternative to viral culture for the diagnosis of perinatal HIV-1 infection. In the second study, I tested prospectively 30 newborns at birth, 4–9 weeks, and 6–9 months: diagnosis of HIV infection by PCR was possible at birth for 3 infants compared with 12 one month later. No changes in the results were observed between the second and third testing. I conclude that, in most cases, PCR will not detect the presence of HIV-1 proviral DNA at birth, but it is a powerful technique that can be used for the diagnosis of perinatal HIV-1 infection from the second month of life.
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© 1992 Springer-Verlag Berlin Heidelberg
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Krivine, A. (1992). Human Immunodeficiency Virus Type 1 (HIV-1) Detection by Polymerase Chain Reaction in Children of Infected Mothers. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_2
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DOI: https://doi.org/10.1007/978-3-642-84766-0_2
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