Advertisement

Determination of Size and Structure of Lipid IVA Vesicles by Quasi-Elastic Light Scattering and Small-Angle X-Ray Scattering

  • N. Maurer
  • O. Glatter
Conference paper
Part of the Springer Proceedings in Physics book series (SPPHY, volume 66)

Abstract

Heavy sonication of lipid IVA in different buffer solutions at pH values around 7.4 produces mostly unilamellar vesicles, which are stable over several weeks. For short sonication times these vesicles are very large, about 250 nanometers in terms of an average hydrodynamic radius with a slight polydispersity, as determined by quasi-elastic light scattering. Therefore small-angle X-ray scattering curves obtained with a conventional instrument do not contain enough information to determine the overall size of the lipid IVA aggregates. However, the pair distance distribution function (PDDF) of the scattering curve is in agreement with the assumption of large vesicular particles. The thickness of the double layer is very small compared to the overall dimension. It can be evaluated assuming a lamellar particle. The corresponding PDDF provides a thickness of about 5 nm. The electron density distribution within the double layer can be calculated directly from the PDDF by a convolution square root operation. Head groups and hydrocarbon chains are represented by two regions of different electron densities.

Keywords

Hydrocarbon Chain Full Line Electron Density Distribution Cylindrical Micelle Conventional Instrument 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    E. Th. Rietschel: Chemistry of Endotoxin, Vol. 1, Ser. Handbook of Endotoxin, ed. R.A. Proctor (Elsevier, 1984)Google Scholar
  2. 2.
    R. Pecora: Dynamic light scattering (Plenum Press, N.Y. 1985)CrossRefGoogle Scholar
  3. 3.
    O. Glatter, O. Kratky: Small-angle X-ray scattering (Academic Press, London 1982)Google Scholar
  4. 4.
    O. Glatter: J. Appl. Cryst. 10, 415–421 (1977)CrossRefGoogle Scholar
  5. 5.
    O. Glatter: J. Appl. Cryst. 14, 101–108 (1981)CrossRefGoogle Scholar
  6. 6.
    O. Glatter, B. Hainish: J. Appl. Cryst. 17, 435–441 (1984)CrossRefGoogle Scholar
  7. 7.
    O. Glatter: J. Appl. Cryst. 21, 886–890 (1988)CrossRefGoogle Scholar
  8. 8.
    O. Glatter: J. Appl. Cryst. 13, 577–584 (1980)CrossRefGoogle Scholar
  9. 9.
    M. Hofer, R.Y. Hampton, C.R.H. Raetz, H. Yu submitted to: Chem. Phys. Lipids (1991)Google Scholar
  10. 10.
    O. Glatter: Progr. Colloid. Polym. Sci. 84, in press (1991)Google Scholar
  11. 11.
    N. Maurer, O. Glatter: J. Appl. Cryst. 24, in press (1991)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • N. Maurer
    • 1
  • O. Glatter
    • 1
  1. 1.Institute of Physical ChemistryUniversity of GrazGrazAustria

Personalised recommendations