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Vesicle-Substrate Interaction Studied by Reflection Interference Contrast Microscopy

  • J. Rädler
  • E. Sackmann
Part of the Springer Proceedings in Physics book series (SPPHY, volume 66)

Abstract

Giant DMPC-Vesicles interacting with a supported DMPC bilayer are investigated by Reflection Interference Contrast Microscopy (RICM). Spherical vesicles whose shape fluctuations are suppressed by osmotic pressure can be observed to fluctuate like Brownian particles above the substrate. The interaction potential can be determined from the measured distribution function of distances. In the case of adhesion, the contact contour of deflated vesicles can be calculated. Contact roundings for of weak adhesion as well as contact angles for strong adhesion have been measured.

Keywords

Contact Angle Equilibrium Shape Adhesion Energy Object Beam Weak Adhesion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    Kaes, J. and Sackmann, E., Bio. Phys J. 60 (1991) 1Google Scholar
  2. [2]
    Seifert, U. and Lipowsky, R., Phys.Rev A 42 (1990) 4768CrossRefGoogle Scholar
  3. [3]
    Raedler, J. and Sackmann, E. submittedGoogle Scholar
  4. [4]
    Prieve, D.C. and Frey, N.A., Langmuir 6 (1990) 396CrossRefGoogle Scholar
  5. [5]
    Evans, E.A. and Parsegian, V.A., in Surface Phenomena in Hemorheology: Copley, A.L. and Seaman, G.V.F., Eds., N.Y. Acad. Sci. (1983) 13Google Scholar
  6. [6]
    Evans, E.A. and Metcalf, M., Biophys. J. 46 (1984) 423CrossRefGoogle Scholar
  7. [7]
    Marra, J. and Israelachvili, J., Biochemistry 24 (1985) 4608CrossRefGoogle Scholar
  8. [8]
    Servuss, R.M. and Helfrich, W. J.Phys. France 50 (1989) 809CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J. Rädler
    • 1
  • E. Sackmann
    • 1
  1. 1.Physik Department, Biophysics Group E22Technische Universität MünchenGarchingFed. Rep. of Germany

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