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Vesicle-Substrate Interaction Studied by Reflection Interference Contrast Microscopy

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The Structure and Conformation of Amphiphilic Membranes

Part of the book series: Springer Proceedings in Physics ((SPPHY,volume 66))

Abstract

Giant DMPC-Vesicles interacting with a supported DMPC bilayer are investigated by Reflection Interference Contrast Microscopy (RICM). Spherical vesicles whose shape fluctuations are suppressed by osmotic pressure can be observed to fluctuate like Brownian particles above the substrate. The interaction potential can be determined from the measured distribution function of distances. In the case of adhesion, the contact contour of deflated vesicles can be calculated. Contact roundings for of weak adhesion as well as contact angles for strong adhesion have been measured.

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References

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Author information

Authors and Affiliations

  1. Physik Department, Biophysics Group E22, Technische Universität München, James Franck-Str., W-8046, Garching, Fed. Rep. of Germany

    J. Rädler & E. Sackmann

Authors
  1. J. Rädler
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  2. E. Sackmann
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Editor information

Editors and Affiliations

  1. Institut für Festkörperforschung Forschungszentrum Jülich, W-5170, Jülich, Fed. Rep. of Germany

    Reinhard Lipowsky , Dieter Richter  & Kurt Kremer ,  & 

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© 1992 Springer-Verlag Berlin Heidelberg

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Rädler, J., Sackmann, E. (1992). Vesicle-Substrate Interaction Studied by Reflection Interference Contrast Microscopy. In: Lipowsky, R., Richter, D., Kremer, K. (eds) The Structure and Conformation of Amphiphilic Membranes. Springer Proceedings in Physics, vol 66. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84763-9_30

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  • DOI: https://doi.org/10.1007/978-3-642-84763-9_30

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84765-3

  • Online ISBN: 978-3-642-84763-9

  • eBook Packages: Springer Book Archive

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