Vesicle-Substrate Interaction Studied by Reflection Interference Contrast Microscopy
Giant DMPC-Vesicles interacting with a supported DMPC bilayer are investigated by Reflection Interference Contrast Microscopy (RICM). Spherical vesicles whose shape fluctuations are suppressed by osmotic pressure can be observed to fluctuate like Brownian particles above the substrate. The interaction potential can be determined from the measured distribution function of distances. In the case of adhesion, the contact contour of deflated vesicles can be calculated. Contact roundings for of weak adhesion as well as contact angles for strong adhesion have been measured.
KeywordsContact Angle Equilibrium Shape Adhesion Energy Object Beam Weak Adhesion
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