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Electron Microscopy of Biological Model Membranes

  • Conference paper
The Structure and Conformation of Amphiphilic Membranes

Part of the book series: Springer Proceedings in Physics ((SPPHY,volume 66))

Abstract

Cryo-electron microscopy was used to study the appearance of vesicles of phosphatidylcholine. From the micrographs we concluded that lateral tension was produced during the freezing process. Membrane undulations were flattened and also the tension forced the vesicles to change their shapes. However, proper preparation techniques led to the finding of vesicles with grainy membrane surfaces or with pronounced edges or bulges. This supported the idea that unstressed membranes may tend to develop an optically unresolvable superstructure capable of consuming large amounts of membrane area.

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Author information

Authors and Affiliations

  1. Fachbereich Physik, Freie Universität Berlin, Arnimallee 14, W-1000, Berlin 33, Fed. Rep. of Germany

    B. Klösgen & W. Helfrich

Authors
  1. B. Klösgen
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  2. W. Helfrich
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Editor information

Editors and Affiliations

  1. Institut für Festkörperforschung Forschungszentrum Jülich, W-5170, Jülich, Fed. Rep. of Germany

    Reinhard Lipowsky , Dieter Richter  & Kurt Kremer ,  & 

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© 1992 Springer-Verlag Berlin Heidelberg

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Klösgen, B., Helfrich, W. (1992). Electron Microscopy of Biological Model Membranes. In: Lipowsky, R., Richter, D., Kremer, K. (eds) The Structure and Conformation of Amphiphilic Membranes. Springer Proceedings in Physics, vol 66. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84763-9_22

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  • DOI: https://doi.org/10.1007/978-3-642-84763-9_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84765-3

  • Online ISBN: 978-3-642-84763-9

  • eBook Packages: Springer Book Archive

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