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Recent advances in the molecular biology of Cryptococcus neoformans

  • Jeffrey C. Edman
Conference paper
Part of the NATO ASI Series book series (volume 69)

Abstract

Cryptococcus neoformans is the causative agent of cryptococcosis. Once a rare disease seen only in the terminally ill, it became much more common with the advent of immunosuppressive regimens for the treatment of neoplastic disease and for transplant maintenance. The disease has taken on added importance as one of the most common opportunistic infections seen in patients with AIDS. Anti-cryptococcal therapy is difficult in AIDS patients due to decreased cure rates and increased relapse rates. This increased incidence of cryptococcosis has lead to increased interest in the mechanism of pathogenesis and virulence.

Keywords

Cryptococcus Neoformans Telomere Sequence Homologous Integration URA5 Gene Opposite Mating Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bhattacharjee AK, Kwon-Chung KJ and Glaudemans CPJ (1979) The structure of the capsular polysaccharide of Cryptococcus neoformans serotype D. Carbohydr Res 73: 183–192.PubMedCrossRefGoogle Scholar
  2. Blackburn EH (1990) Telomeres: structure and synthesis. J Biol Chem 265: 5919–5921.PubMedGoogle Scholar
  3. Cherniak R (1988) Soluble polysaccharides of Cryptococcus neojormans. Curr Top Med Mycol 2: 40–54.PubMedCrossRefGoogle Scholar
  4. Collins J (1981) Instability of palindromic DNA in E coli. Cold Spring Harbor Symp Quant Biol 45: 409.PubMedGoogle Scholar
  5. Edman JC (1992) Isolation of telomere-like sequences from Cryptococcus neoformans and their use in high-efficiency transformation. Mol Cell Biol 12:2777–2783.PubMedGoogle Scholar
  6. Edman JC and Kwon-Chung KJ (1990) Isolation of the URA5gene from Cryptococcus neoformans var neoformans and its use as a selective marker for transformation. Mol Cell Biol 10: 4538–4544.PubMedGoogle Scholar
  7. Fincham JRS (1989) Transformation in Fungi. Microbiol Rev 53: 148–170.PubMedGoogle Scholar
  8. Fromtling RA, Shadomy HJ and Jacobson ES (1982) Decreased virulence in stable, acapsular mutants of Cryptococcus neoformans. Mycopathologica 79: 23–29.CrossRefGoogle Scholar
  9. Jacobson, ES, Ayers DJ, Harrell AC and Nicholas CC (1982) Genetic and phenotypic characterization of capsule mutants of Cryptococcus neoformans. J Bacteriol 150: 1292–1296.PubMedGoogle Scholar
  10. Kwon-Chung KJ (1975) A new genus, Filobasidiella, the perfect state of Cryptococcus neoformans. Mycologia 67: 1197–1200.PubMedCrossRefGoogle Scholar
  11. Kwon-Chung KJ (1977) Heterothallism vs self-fertile isolates of Filobasidiella neoformans (Cryptococcus neoformans) in The black and white yeasts Pan American Health Org Sci Pub 356 Proceedings of the IV Int Conf on Mycoses Washington, DC pp 204-213.Google Scholar
  12. Kwon-Chung KJ (1980) Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans), Mycologia 72: 418–422.PubMedCrossRefGoogle Scholar
  13. Kwon-Chung KJ and Edman JC (1992) Recent advances in the molecular genetics of Cryptococcus neoformans in New Strategies in Fungal Disease. Churchill Livingstone London pp 195-207.Google Scholar
  14. Kwon-Chung KJ, Edman JC and Wickes BL (1992) Genetic association of mating types and virulence in Cryptococcus neoformans. Infect Immun 60: 602–605.PubMedGoogle Scholar
  15. Kwon-Chung KJ, Polachek I and Popkin TJ (1982) Melanin-lacking mutants of Cryptococcus neoformans and their virulence for mice. J Bacteriol 150: 1414–1421.PubMedGoogle Scholar
  16. Kwon-Chung KJ and Rhodes JC (1986) Encapsulation and melanin formation as indicators of virulence in Cryptococcus neoformans. Infect Immun 51: 218–233.PubMedGoogle Scholar
  17. Littman ML and Tsubura E (1959) Effect of degree of encapsulation upon virulence of Cryptococcus neoformans. Proc Soc Exp Biol Med 101: 773–777.PubMedGoogle Scholar
  18. Polacheck I, Hearing VJ and Kwon-Chung KJ (1982) Biochemical studies of phenoloxidase and utilization of catecholamines in Cryptococcus neoformans. J Bacteriol 150: 1212–1220.PubMedGoogle Scholar
  19. Still CN and Jacobson ES (1983) Recombinational mapping of capsule mutations in Cryptococcus neoformans. J Bacteriol 156: 460–462.PubMedGoogle Scholar
  20. Varma A, Edman JC and Kwon-Chung KJ (1992) Molecular and genetic analysis of URA5 transformants of Cryptococcus neoformans. Infect Immun 60:1101–1108.PubMedGoogle Scholar
  21. Whelan WL (1987) The genetics of medically important fungi. Crit Rev Microbiol 14: 99–170.PubMedCrossRefGoogle Scholar
  22. Whelan WL and Kwon-Chung KJ (1986) Genetic complementation in Cryptococcus neoformans. J Bacteriol 166: 924–929.PubMedGoogle Scholar
  23. White CW and Jacobson ES (1985) Occurrence of diploid strains of Cryptococcus neoformans. J Bacteriol 161: 1231–1232.PubMedGoogle Scholar
  24. Zakian VA (1989) Structure and function of telomeres. Ann Rev Genet 23: 579–604.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Jeffrey C. Edman
    • 1
  1. 1.Hormone Research Institute & Department of Laboratory MedicineUniversity of CaliforniaSan FranciscoUSA

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