Molecular Cloning of Nurse Shark cDNAs with High Sequence Similarity to Nucleoside Diphosphate Kinase Genes

  • Masanori Kasahara
  • Camilo Canel
  • E. Churchill McKinney
  • Martin F. Flajnik
Part of the NATO ASI Series book series (volume 59)

Abstract

In the course of our study aimed at isolating class II genes of the major histocompatibility complex from primitive vertebrates, nurse shark cDNA clones assumed to encode a nucleoside diphosphate kinase were isolated. The deduced amino acid sequence of the putative nurse shark nucleoside diphosphate kinase protein has two cysteinyl residues approximately 60 amino acid residues apart from each other. Weak amino acid sequence similarity was found between the putative nurse shark nucleoside diphosphate kinase and C1-SET members of the immunoglobulin superfamily.

Keywords

Codon Leukemia Mold Polysaccharide Fractionation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bevilacqua, G., Sobel, M.E., Liotta, L.A., and Steeg, P.S.: Association of low nm23 RNA levels in human primary infiltrating ductal breast carcinomas with lymph node involvement and other histopathological indicators of high metastatic potential. Cancer Res 49: 5185–5190, 1989PubMedGoogle Scholar
  2. Biggs, J., Tripoulas, N., Hersperger, E., Dearolf, C., and Shearn, A.: Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila. Genes & Dev 2: 1333–1343, 1988CrossRefGoogle Scholar
  3. Biggs, J., Hersperger, E., Steeg, P.S., Liotta, L.A., and Sheam, A.: A Drosophila gene that is homologous to a mammalian gene associated with tumor metastasis codes for a nucleoside diphosphokinase. Cell 63: 933–940, 1990PubMedCrossRefGoogle Scholar
  4. Brutlag, D.L., Dautricourt, J.-P., Maulik, S., and Relph, J.: Improved sensitivity of biological sequence database searches. CABIOS 6: 237–245, 1990PubMedGoogle Scholar
  5. Chomczynski, P. and Sacchi, N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159, 1987PubMedCrossRefGoogle Scholar
  6. Frohman, M.A., Dush, M.K., and Martin, G.R.: Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8998–9002, 1988PubMedCrossRefGoogle Scholar
  7. Hanks, S.K., Quinn, A.M., and Hunter, T.: The protein kinase family: Conserved feature and deduced phylogeny of the catalytic domains. Science 241: 42–52, 1988PubMedCrossRefGoogle Scholar
  8. Hunkapiller, T. and Hood, L.: Diversity of the immunoglobulin gene superfamily. Adv Immunol 44: 1–63, 1989PubMedCrossRefGoogle Scholar
  9. Kimura, N., Shimada, N., Nomura, K., and Watanabe, K.: Isolation and characterization of a cDNA clone encoding rat nucleoside diphosphate kinase. J Biol Chem 265: 15744–15749, 1990PubMedGoogle Scholar
  10. Kokubu, F., Hinds, K., Litman, R., Shamblott, M.J., and Litman, G.W.: Extensive families of constant region genes in a phylogenetically primitive vertebrate indicate an additional level of immunoglobulin complexity. Proc Natl Acad Sci USA 84: 5868–5872, 1987PubMedCrossRefGoogle Scholar
  11. Lacombe, M.-L., Wallet, V., Troll, H., and Veron, M.: Functional cloning of a nucleoside diphosphate kinase from Dictyostelium discoideum. J Biol Chem 265: 10012–10018, 1990PubMedGoogle Scholar
  12. Liotta, L.A. and Steeg, P.S.: Clues to the function of Nm23 and Awd proteins in development, signal transduction, and tumor metastasis provided by studies of Dictyostelium discoideum. J Natl Cancer Inst 82: 1170–1172, 1990PubMedCrossRefGoogle Scholar
  13. Muñoz-Dorado, J., Inouye, M., and Inouye, S.: Nucleoside diphosphate kinase from Myxococcus xanthus. I. Cloning and sequencing of the gene. J Biol Chem 265: 2702–2706, 1990aPubMedGoogle Scholar
  14. Muñoz-Dorado, J., Inouye, S., and Inouye, M.: Nucleoside diphosphate kinase from Myxococcus xanthus. II. Biochemical characterization. J Biol Chem 265: 2707–2712, 1990bPubMedGoogle Scholar
  15. Parks, R.E., Jr. and Agarwal, R.P.: Nucleoside diphosphokinases. In P.D. Boyer (ed.): The Enzymes, Vol. 8, pp. 307–334. Academic Press, New York 1973Google Scholar
  16. Rosengard, A.M., Krutzsch, H.C., Sheam, A., Biggs, J.R., Barker, E., Margulies, I.M.K., King, C.R., Liotta, L.A., and Steeg, P.S.: Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development. Nature 342: 177–180, 1989PubMedCrossRefGoogle Scholar
  17. Sanger, F., Nicklen, S., and Coulson, A.R.: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977PubMedCrossRefGoogle Scholar
  18. Steeg, P.S., Bevilacqua, G., Kopper, L., Thorgeirsson, U.P., Talmadge, J.E., Liotta, L.A., and Sobel, M.E.: Evidence for a novel gene associated with low tumor metastatic potential. J Natl Cancer Inst 80: 200–204, 1988PubMedCrossRefGoogle Scholar
  19. Wallet, V., Mutzel, R., Troll, H., Barzu, O., Wurster, B., Véron, M., and Lacombe, M.-L.: Dictyosterium nucleoside diphosphate kinase highly homologous to Nm23 and Awd proteins involved in mammalian tumor metastasis and Drosophila development. J Natl Cancer Inst 82: 1199–1202, 1990PubMedCrossRefGoogle Scholar
  20. Wierenga, R.K. and Hol, W.G.J.: Predicted nucleotide-binding properties of p21 protein and its cancer-associated variant. Nature 302: 842–844, 1983PubMedCrossRefGoogle Scholar
  21. Williams, A.F. and Barclay, A.N.: The immunoglobulin superfamily - Domains for cell surface recognition. Annu Rev Immunol 6: 381–405, 1988PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Masanori Kasahara
    • 1
  • Camilo Canel
    • 1
  • E. Churchill McKinney
    • 1
  • Martin F. Flajnik
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Miami School of MedicineMiamiUSA

Personalised recommendations