Endocrine Pituitary Cell Cultures: Cellular Morphology, Protein Secretion, and Susceptibility to Weak Bases and Ionophores

  • Athanassios Sambanis
Conference paper
Part of the NATO ASI Series book series (volume 64)


Animal cells are of significant use in bioprocessing technology, tissue engineering, and gene therapy applications. In bioprocess technology, cells cultured in bioreactors produce specific recombinant or, occasionally, endogenous proteins. Compared to bacteria and yeast, animal cells have the disadvantages of slow growth to relatively low densities, and of complex and expensive culture media requirements. On the other hand, they offer the distinct advantage of possessing the enzymatic machinery necessary for performing post-translational modifications on recombinant proteins. Such modifications are essential for biological activity of a protein product and may not be feasible in yeast or, even more so, in bacteria. Animal cells are thus indispensable for production of pharmaceuticals such as tissue plasminogen activator (tPA), factor VIII, or other complex proteins.


Secretory Granule Endocrine Cell Secretory Vesicle Signal Recognition Particle Complete Growth Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Athanassios Sambanis
    • 1
  1. 1.School of Chemical EngineeringGeorgia Institute of TechnologyAtlantaUSA

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