Flow Cytometry pp 343-354 | Cite as

Cytometry, Antitumour Drugs and DNA Topoisomerases

  • P. J. Smith
Conference paper
Part of the NATO ASI Series book series (volume 67)

Summary

Flow cytometry has a significant role to play in the of study cellular factors that determine the cytotoxic potential of anticancer drugs. This brief overview provides specific examples of techniques for the analysis of: drug uptake, nuclear localization of drug molecules and drug-induced cell death. Emphasis has been placed on the study of anticancer drugs recognised as potent DNA topoisomerase II poisons together with examples of how target enzyme expression can be analysed in single cells with respect to cell cycle age. An important advantage of the single cell analytical techniques described is the ability to distinguish population heterogeneity, often a confounding factor in the analysis of human tumour specimens.

Keywords

Lymphoma Iodide Glutamine Alkaloid Ethidium 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • P. J. Smith
    • 1
  1. 1.Medical Research Council Clinical Oncology and Radiotherapeutics UnitMRC CentreCambridgeUK

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