Cytometry, Antitumour Drugs and DNA Topoisomerases
Flow cytometry has a significant role to play in the of study cellular factors that determine the cytotoxic potential of anticancer drugs. This brief overview provides specific examples of techniques for the analysis of: drug uptake, nuclear localization of drug molecules and drug-induced cell death. Emphasis has been placed on the study of anticancer drugs recognised as potent DNA topoisomerase II poisons together with examples of how target enzyme expression can be analysed in single cells with respect to cell cycle age. An important advantage of the single cell analytical techniques described is the ability to distinguish population heterogeneity, often a confounding factor in the analysis of human tumour specimens.
KeywordsLymphoma Iodide Glutamine Alkaloid Ethidium
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