Cytometry, Antitumour Drugs and DNA Topoisomerases
Flow cytometry has a significant role to play in the of study cellular factors that determine the cytotoxic potential of anticancer drugs. This brief overview provides specific examples of techniques for the analysis of: drug uptake, nuclear localization of drug molecules and drug-induced cell death. Emphasis has been placed on the study of anticancer drugs recognised as potent DNA topoisomerase II poisons together with examples of how target enzyme expression can be analysed in single cells with respect to cell cycle age. An important advantage of the single cell analytical techniques described is the ability to distinguish population heterogeneity, often a confounding factor in the analysis of human tumour specimens.
KeywordsMedian Fluorescence Intensity Lx104 Cell Antitumour Drug Cell Cycle Dependency Human Tumour Specimen
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- Liu LF (1989) DNA topoisomerase poisons as anti-tumour drugs. Ann Rev Biochem 58:351–375Google Scholar
- Lown JW, Hanstock CC (1985) High field 1H-NMR analysis of the 1:1 intercalation complex of the antitumor agent mitoxantrone and the DNA duplex [d(CpGpCpGp)]2- J Biomol Struct Dynam 2: 1097–1106Google Scholar
- Smith PJ (1990) DNA topoisomerase dysfunction: a new goal for antitumor chemotherapy. Bio Essays 12: 167–172Google Scholar
- Wang JC(1985) DNA topoisomerases. Annu Rev Biochem 54:665–697Google Scholar