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Energy Transfer

  • Conference paper
Flow Cytometry

Part of the book series: NATO ASI Series ((ASIH,volume 67))

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Abstract

In most instances classification of biological cells is based on a morphological description. This was established by conventional microscopy using different staining procedures and in many instances still is the prime source for the discrimination of different cell types. However, staining reveals or highlights the spatial organization of mostly intracellular chemistry and biochemistry. Antibodies raised against different protein structures and labeled with various chromophores increased the level of discrimination of different cell classes even further. In most cases, cytometry uses the very same principles to discriminate different cell types. Cells are stained and/or labeled (after fixation) and the intensity of emitted fluorescence light is measured. Based on the assumption, that fluorescence emission intensity is proportional to the number of molecules bound and binding is stoichiometric to molecules/structures of interest, a quantitative analysis of cell constituents serves as discrimination of cell classes or subclasses.

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Author information

Authors and Affiliations

  1. Department of Biophysics, University of Hannover, Germany

    Wolfgang G. Eisert

  2. Department of Clinical Pharmacological Research, Dr. Karl Thomae GmbH, W-7950, Biberach/Riß, Germany

    Wolfgang G. Eisert

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  1. Wolfgang G. Eisert
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Editors and Affiliations

  1. Laboratoire de Cytométrie, Centre National de la Recherche Scientifique, B. P. 3, 94801, Villejuif, France

    Alain Jacquemin-Sablon

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© 1993 Springer-Verlag Berlin Heidelberg

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Eisert, W.G. (1993). Energy Transfer. In: Jacquemin-Sablon, A. (eds) Flow Cytometry. NATO ASI Series, vol 67. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84616-8_12

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  • DOI: https://doi.org/10.1007/978-3-642-84616-8_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84618-2

  • Online ISBN: 978-3-642-84616-8

  • eBook Packages: Springer Book Archive

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