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Cancer Therapy pp 109-120 | Cite as

Hemin is Transported in Human Leukemia K562 Cells and Interacts with DNA Sequences

  • Asterios S. Tsiftsoglou
  • Athina I. Tsamadou
  • Stephen H. Robinson
  • Willie Wong
Part of the NATO ASI Series book series (volume 75)

Abstract

Heme (ferroprotoporphyrin IX), a natural agent, serves as a prosthetic group in various hemoproteins like hemoglobin, myoglobin, cytochromes, catalase and others involved in oxygen transport, cellular respiration, ATP produetion and drug metabolism (Stryer, 1988). Hemin, the oxidised form of heme, has been shown to activate gene expression and promote differentiation in a variety of cell types including mouse 3T3 cells (Chen and London, 1981), neuroblastoma (Ishii et al, 1978), erythroleukemia (MEL, K562) cells (Ross et al, 1976; Rutherford et al, 1979a; Tsiftsoglou and Robinson, 1985; Tsiftsoglou et al, 1991) as well as normal hematopoietic cells like CFU-E, CFU-GM and BFU-E (Monette et al, 1982) by interacting with cellular components at various levels (Sassa et al, 1988).

Keywords

K562 Cell Globin Gene Local Control Region Erythroleukemia Cell Hemoglobin Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abraham NG (1992) Heme Regulation of hematopoietic stem cell growth and development. In “Concise Reviews in ExDerimental Hematology” Ed. M.J. Murphy, Alpha Press, N. York, pp 357–373Google Scholar
  2. Benz EJ, Muräne MJ, Tonkonow BL, Berman BW, Mazur EM, Cavellesco C, Jenko T, Snyder EL, Forget BG, Hoffman R (1980) Embryonic-fetal erythroid characteristics of a human leukemic cell line. Proc. Natl. Acad. Sci. USA 77: 3509PubMedCrossRefGoogle Scholar
  3. Charney P and Maniatis T (1983) Transcriptional regulation of globin gene expression in the human erythroid cell line K562. Science 220:1281,CrossRefGoogle Scholar
  4. Chen IT and London IM (1981) Hemin enhances the differentiation of mouse 3T3 cells to adipocyte. Cell 26: 117PubMedCrossRefGoogle Scholar
  5. Dean A, Erard F, Schneider A, Schechter A (1981) Induction of hemoglobin accumulation in human K562 cells by hemin is reversible. Science 212: 459PubMedCrossRefGoogle Scholar
  6. Galbraith RF, Sassa S, Kappas A (1985) Heme binding to murine erythroleukemia cells. Evidence for a heme receptor. J. Biol. Chemistry 260:12198Google Scholar
  7. Garner M, and Revzin A (1981) A gel electrophoresis method for qualifying the binding of proteins to specific DNA regions:applications to components of the Escherichia Coli lactose operon regulatory system. Nucl. Acid Research, 9: 3047–3060CrossRefGoogle Scholar
  8. Guarente L, Mason T (1983) Heme regulates transcription of the CYC1 gene of S. Cerevisiae via an upstream activation site. Cell 32: 1279–1282PubMedCrossRefGoogle Scholar
  9. Gusella JF, Weil S, Tsiftsoglou AS, Volloch V, Newman J, Keys C, Housman D (1980) Hemin does not cause commitment of MEL cells. Blood 56: 481PubMedGoogle Scholar
  10. Ishii 0M, Maniatis G (1978) Hemin promotes rapid neurite outgrowth in cultured mouse neuroblastoma cells. Nature, 274: 372Google Scholar
  11. Lawn RM, Efstratiadis A, O’Connell C, Maniatis T (1980) The nucleotide sequence of the human /?-globin gene Cell 21: 647–651PubMedCrossRefGoogle Scholar
  12. Lozzio CB, Lozzio BB (1975) Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome. Blood 45:321,PubMedGoogle Scholar
  13. London IM, v Ernst, Fagard R, Leroux A, Levin DH, Petryshyn R (1981) Regulation of protein synthesis by phosphorylation and heme. Cold Spring Harbor Conferences on cell Droliferation, 8: Prote in phosphorylationGoogle Scholar
  14. Maniatis T, Fritsch FF, Sambrook J (1982) Molecular Cloning “A Laboratory Manual” Cold Spring Harbor New York: Cold Spring Harbor Laboratory pp 180Google Scholar
  15. Monette FC and Holden SA (1982) Hemin enhances the in vitro growth of primitive erythroid progenitor cells. Blood 60: 527PubMedGoogle Scholar
  16. Muller-Eberhard U and Nikkila H (1989) Transport of tetrapyrroles by proteins. Seminars in Hematology 26: 86PubMedGoogle Scholar
  17. Orkin SH, (1990) Globin regulation and switching: Circa 1990. Cell 63: 665–672PubMedCrossRefGoogle Scholar
  18. Ross J and Sautner D (1976) Induction of globin mRNA accumulation by hemin in cultured erythroleukemia cells. Cell 8: 513PubMedCrossRefGoogle Scholar
  19. Rowely DT, Ohlsson-Wilhelm BM, Farley BA (1985) K562 human erythro-leukemia cells demonstrate commitment. Blood 65: 862Google Scholar
  20. Rutherford TR, Clegg JB, Weatherall DJ (1979a) K562 human leukemic cells synthesise embryonic hemoglobin in response to hemin. Nature 280: 164, 1979Google Scholar
  21. Rutherford and Weatherall DJ (1979b) Deficient heme synthesis as the cause of non-inducibi1ity of hemoglobin synthesis in a Friend Erythroleukemia cell line. Cell 16: 415–423Google Scholar
  22. Sassa S (1988) Heme Stimulation of cellular growth and differentiation. Seminars in Hematology 25: 312PubMedGoogle Scholar
  23. Stryer L. (1988) Oxygen transporting proteins: Myoglobin and hemoglobin. In “Biochemistry” 3rd Edition, W.H. Freeman and Company, N. York, p 144,Google Scholar
  24. Treisman R, Orkin SH, Maniatis T (1983) Specific transcription and DNA splicing defects in five cloned 6-thalassemia genes Nature 302: 591–596PubMedCrossRefGoogle Scholar
  25. Tsamadou AI, Wong W and Tsiftsoglou AS (1992) Hemin uptake and detection of hemin binding proteins (HeBP) in human leukemia K562 cells. In “Metal Ions in Biology and Medicine” Vol. 2 Eds J. Anastassopoulou, Ph.Collery, JC Etienne, T. Theophanides, John Libbey Eurotext, Paris pp 145–150Google Scholar
  26. Tsiftsoglou As, Bhargava KK, Rittman LS, Sartorelli AC (1981) Distribution of the inducer of differentiation Bis-Acetyl-diaminopentane in Murine erythroleukemia Cells J. of Cell. Physiol, 106: 419–424CrossRefGoogle Scholar
  27. Tsiftsoglou AS and Robinson SH (1985) Differentiation of leukemia cell lines: a review of focusing on murine erythroleukemia and human HL-60 cells. Int. J. Cell Cloning 3: 349PubMedCrossRefGoogle Scholar
  28. Tsiftsoglou AS, Wong W, Robinson SH, Hensold J (1989) Hemin increases production of ß-like globin RNA transcripts in human erythroleukemia K562 cells. Develop. Genetics 10: 311CrossRefGoogle Scholar
  29. Tsiftsoglou AS, Wong W, Tsamadou AI, Robinson SH (1991) Cooperative effects of hemin and anthracyclines in promoting terminal erythroid maturation in K562 human erythroleukemia cells. Exp. Hematol. 19: 928PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Asterios S. Tsiftsoglou
    • 1
  • Athina I. Tsamadou
    • 1
  • Stephen H. Robinson
    • 2
  • Willie Wong
    • 2
  1. 1.Laboratory of Pharmacology, Department of Pharmaceutical SciencesAristotle University of ThessalonikiThessalonikiGreece
  2. 2.Department of MedicineBeth Israel Hospital and Harvard Medical SchoolBostonUSA

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