Molecular Analysis of Isochromosome 12p in Testicular Germ Cell Tumors
Testicular germ cell tumors (TGCTs) of adults are marked by a common structural chromosomal abnormality: i(12p) (Atkin and Baker 1988). This chromosome anomaly is characteristic for all histologic varieties of TGCTs and is observed in more than 80% of all cases. This frequent occurrence suggests that the altered chromosome 12 could play an important role in the patho- genesis of germ cell tumors. However, at the present time little is known about the role of this chromosome in tumorigenesis. In addition, TGCTs are usually highly aneuploid. Thus, besides i(12p) formation, aneuploidization could play an important role in the oncogenesis of TGCTs. We supply evidence suggesting that i(12p) formation is not the first step in the develop-ment of TGCTs and that its generation does not lead to gross loss of material of the long arm of chromosome 12. We also show that in situ hybridization (“chromosome painting”) represents a valuable new approach for the detection of the i(12p) chromosome in germ cell tumor material.
KeywordsGerm Cell Tumor Embryonal Carcinoma Chromosome Painting Restriction Fragment Length Polymorphism Analysis Testicular Germ Cell Tumor
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