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Ultrastructural Immunocytochemical Localization of Choline Acetyltransferase in Sympathetic Ganglia

  • Margaret R. Matthews
  • A. Ribeiro-da-Silva
  • A. Claudio Cuello
Part of the NATO ASI Series book series (volume 58)

Abstract

In the central nervous system the demonstration of neurones and nerve terminals immunoreactive for choline acetyltransferase (ChAT) has been successfully achieved by a number of groups, using several different primary antibodies, and has already almost the status of a routine procedure. In the peripheral nervous system the immunolocalization of this transmitter- synthesizing enzyme has not been entirely straightforward, as the antibodies and procedures which are effective in central nervous tissue do not always prove so efficacious in the periphery. Lindh et al. (1986) and Landis et al. (1987) have recently demonstrated immunolocalization of the enzyme in nerve terminal networks in peripheral autonomic ganglia at the light microscope level. We here report its immunocytochemical, ultrastructural localization, in nerve fibres and nerve endings of sympathetic ganglia of the rat.

Keywords

Nerve Terminal Sympathetic Ganglion Superior Cervical Ganglion Choline Acetyltransferase Central Nervous Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Leblanc GG, Trimmer BA, Landis SC (1987) Neuropeptide Y-like immunoreactivity in rat cranial parasympathetic neurons: coexistence with vasoactive intestinal peptide and choline acetyltransferase. Proc natl Acad Sci USA 84: 3511–3515PubMedCrossRefGoogle Scholar
  2. Lindh B, Staines W, Hökfelt T, Terenius L, Salvaterra PM (1986) Immunohistochemical demonstration of choline acetyltransferase- immunoreactive preganglionic nerve fibres in guinea pig autonomic ganglia. Proc natl Acad Sci USA 83: 5316–5320PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Margaret R. Matthews
    • 1
  • A. Ribeiro-da-Silva
    • 2
  • A. Claudio Cuello
    • 2
  1. 1.Department of Human AnatomyUniversity of OxfordOxfordUK
  2. 2.Department of Pharmacology and TherapeuticsMcGill UniversityMontrealCanada

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