Antigen Processing in B Lymphocytes
There is considerable current interest in how the events occurring during antigen processing and presentation via class II MHC glycoproteins are organised with respect to the endocytic pathway. This follows the discovery that most antigens require an intracellular processing step in order to produce a suitable ligand for binding to MHC Class II glycoproteins and, as a complex, to the T cell receptor. The available evidence strongly suggests that following endocytosis, processing and unfolding take place in intracellular compartments and that suitable MHC/peptide complexes are then returned to the cell surface. Using T cells to assay for the appearance of processed determinants it has been established that these antigen processing events take approximately one hour, are sensitive to chloroquine and fixation of the antigen presenting cells (APC) and to inhibitors of endosomal/lysosomal proteases (Ziegler and Unanue 1982; Lanzavecchia 1985; Buus and Werdelin 1986).
KeywordsChloroquine Tetanus Tricine Primaquine
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