Endocytosis pp 17-23 | Cite as

Surface Distribution and Pathway of Internalization of C3b Receptors (CR1) in Human Neutrophils

  • J.-P. Paccaud
  • J.-L. Carpentier
Conference paper
Part of the NATO ASI Series book series (volume 62)


Neutrophils are implicated in the prevention of bacterial infection. To that end, phagocytes must first sense the presence of bacteria, reach the sites of infection and eventually get in close contact with the microorganisms to ingest them (Elsbach P and Weiss J, 1988). This complex process requires the cooperation of several classes of receptors (adherence, chemoattractants, and phagocytic receptors) present on their surface. Chemoattractant receptors, which bind molecules such as C5a or fMLP, are coupled to phosphoinositide hydrolysis which leads to an increase of cytosolic free calcium [Ca2+]i and activation of protein kinase C (Snyderman R et al., 1986). In turn, this signalling cascade is thought to be essential for triggering oriented locomotion and secretion of neutrophils (Stossel TP, 1988). Among phagocytic receptors, those for Fc portion of immunoglobulins are endowed with the intrinsic ability to trigger ingestion and metabolic responses towards antibody coated particles. On the other hand, complement receptors such as CR1 (receptors for C3b), promote the binding of complement activating particles (such as bacteria or immune complexes), and their subsequent phagocytosis, synergestically with Fc receptors (Unkeless JC and Wright SD, 1988). In the case of CR1, the aquisition of the phagocytic capability requires its activation by a process thought to involve phosphorylation of the receptor (Changelian PS and Fearon DT, 1986). Full phagocytic capacity of CR1 is obtained when neutrophils are costimulated with fibronectin or laminin (Pommier et al.,1983; Bohnsack et al. 1985). Pharmacological agents such as PMA also mimic this activation process (Kazatchkine MD and Fearon DT, 1990).


Complement Receptor Inflammation Basic Principle Cytosolic Free Calcium Phagocytic Receptor Chemoattractant Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Berger M, Birx DL, Wetzler EM (1988) Tumor necrosis factor is the major monocyte product that increases complement receptor expression on mature human neutrophils. Blood 71:151–158.PubMedGoogle Scholar
  2. Berger M, Birx DL, Wetzler EM, O’Shea JJ, Brown EJ, Cross AS (1985) Calcium requirements for increased complement receptor expression during neutrophil activation. J. Immunol. 135:1342–1348.PubMedGoogle Scholar
  3. Bohnsack HF, Kleinman HK, Takahashi T, O’Shea JJ, Brown EJ (1985) Connective tissue proteins and phagocytic cell function. Laminin enhances complement and Fc-mediated phagocytosis by cultured human macrophages. J. Exp. Med. 161: 912–923.PubMedCrossRefGoogle Scholar
  4. Carpentier J-L, Lew DP, Paccaud J-P, Gil R, lacopetta B, Kazatchkine M, Stendhal O, Pozzan T (1990) Internalization pathway of C3b receptors in human neutrophils and its transmodulation by chemoattractant receptors stimulation. Cell Regulation (in press).Google Scholar
  5. Changelian PS, Fearon DT (1986) Tissue-specific phosphorylation of complement receptors CR1 and CR2. J. Exp. Med 163:101–115.PubMedCrossRefGoogle Scholar
  6. Edberg JC, Wright E, Taylor RP (1987) Qantitative analysis of the binding of soluble complement-fixing antibody/dNA immune complexes to CR1 on human red blood cells J. Immunol. 139: 3739–3747.PubMedGoogle Scholar
  7. Elsbach P, Weiss J (1988) Phagocytic cells: oxygen-independent antimicrobial systems in: Inflammation basic principles and clinical correlates (eds) Gallin JI, Golstein IM, Snyderman R. Raven Press New York.Google Scholar
  8. Fearon DT, Collins LA (1983) Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J. Immunol. 130:370–375.PubMedGoogle Scholar
  9. Kazatchkine MD, Fearon DT (1990) Deficiencies of human C3 complement receptors type 1 (CR1, CD35) and type 2 (CR2, CD21) Immunodeficiency Rev. 2:17–41.Google Scholar
  10. Madi N, Paccaud J-P, Steiger G, Schifferli JA (1990) Immune complex (IC) binding efficiency of erythrocyte complement receptor type 1 (CR1). Clin. Exp. Immunol, (in press).Google Scholar
  11. Paccaud J-P, Carpentier J-L, Schifferli JA (1988) Direct evidence for the clustered nature of complement receptor type 1 on the erythrocyte membrane. J. Immunol. 141:3889–3894.PubMedGoogle Scholar
  12. Paccaud J-P, Carpentier J-L, Schifferli JA (1990) Difference in the clustering of complement receptor type 1 (CR1) on polymorphonuclear leukocytes and erythrocytes: effect on immune adherence Eur. J. Immunol. 20: 283–289.PubMedCrossRefGoogle Scholar
  13. Paccaud J-P, Schifferli JA, Baggiolini M (1990) NAP-1/IL-8 induces up-regulation of CR1 receptors in human neutrophil leukocytes. Biochem. Biophys. Res. Comm. 166: 187–192.PubMedCrossRefGoogle Scholar
  14. Paccaud J-P, Carpentier J-L, Schifferli JA (1990) Exudation induces clustering of CR1 receptors at the surface of human polymorphonuclear leukocytes. Biochem. Biophys. Res. Comm. (in press).Google Scholar
  15. Pommier CG, O’Shea J, Chused T, Yancey K, Frank MM, Takahashi T, Brown EJ (1984) Studies of the fibronectin receptors of human peripheral blood leucocytes. J. Exp. Med. 159: 137–145.PubMedCrossRefGoogle Scholar
  16. Snyderman R, Smith CD, Verghese MW (1986) Model for leukocyte regulation by chemoattractant receptors: roles of a guanine nucleotide regulatory protein and polyphosphoinositide metabolism. J. Leuko. Biol. 40: 785–800.PubMedGoogle Scholar
  17. Stossel TP (1988) The mechanical responses of white blood cells in: Inflammation basic principles and clinical correlates (eds) Gallin JI, Golstein IM, Snyderman R. Raven Press New York.Google Scholar
  18. Unkeless JC, Wright SD (1988) Phagocytic cells and complement receptors in: Inflammation basic principles and clinical correlates (eds) Gallin JI, Golstein IM, Snyderman R. Raven Press New York.Google Scholar
  19. Wong WW, Cahill JM, Rosen MD, Kennedy CA, Bonaccio ET, Morris MJ, Wilson JG, Klickstein LB, Fearon DT (1989) Structure of the human CR1 gene. Molecular basis of the structural and quantitative polymorphisms and identificaion of a new CR1-like allele. J. Exp. Med. 169: 847–863.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J.-P. Paccaud
    • 1
  • J.-L. Carpentier
    • 1
  1. 1.Institute of Histology and EmbryologyUniversity of Geneva Medical CenterGeneva 4Switzerland

Personalised recommendations