Skip to main content
Book cover

Rice pp 251–268Cite as

Isolation, Culture, and Fusion of Rice Protoplasts

  • Chapter

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 14))

Abstract

Wild species within the genus Oryza and other related graminaceous species offer sources of important genes conferring traits such as salinity tolerance and resistance to pests and diseases (Swaminathan 1986). Since many of the wild species in question are sexually isolated from rice, protoplast fusion offers a unique opportunity to develop previously unobtainable hybrids and cybrids. Furthermore, direct interactions of protoplasts with recombinant plasmids can lead to the incorporation and expression of specific-gene constructs.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/technology 4:1087–1090

    Article  Google Scholar 

  • Abdullah R, Thompson JA, Khush GS, Kaushik RP, Cocking EC (1989) Protoclonal variation in the seed progeny of plants regenerated from rice protoplasts. Plant Science (in press)

    Google Scholar 

  • Ammirato PV (1983) Embryogenesis. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 1. MacMillan, New York, pp 82–123

    Google Scholar 

  • Baba A, Hasezawa S, Syono K (1986) Cultivation of rice protoplasts and their transformation mediated by Agrobacterium spheroplasts. Plant Cell Physiol 27(3):463–471

    CAS  Google Scholar 

  • Bajaj YPS (1983) Survival of somatic hybrid protoplasts of wheat x pea and rice x pea subjected to -196°C. Indian J Exp Biol 21:120–122

    Google Scholar 

  • Chand PK, Davey MR, Power JB, Cocking EC (1988) An improved procedure for protoplast fusion using polyethylene glycol. J Plant Physiol 133:480–485

    CAS  Google Scholar 

  • Chang TT (1985) Crop history and genetic conservation: Rice — a case study. Iowa State J 59(4):425–455

    Google Scholar 

  • Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–668

    Google Scholar 

  • Comai L, Facciotti D, Hiatt WR, Thompson G, Rose RE, Stalker DM (1984) Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature (London) 317:741–744

    Article  Google Scholar 

  • Coulibaly MY, Demarly Y (1986) Regeneration of plantlets from protoplasts of rice, Oryza saliva L. Z Pflanzenzücht 96:79–81

    Google Scholar 

  • de Datta SK (1981) Principles and practices of rice production. John Wiley & Sons, New York London

    Google Scholar 

  • Finch RP, Cocking EC (1989) Dividing heterokaryons produced by fusion of rice (Oryza saliva L.) and Porteresia coarctata protoplasts. (in preparation)

    Google Scholar 

  • Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33:130–137

    Article  PubMed  CAS  Google Scholar 

  • Fromm ME, Taylor LP, Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature (London) 319:791–793

    Article  CAS  Google Scholar 

  • Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult Lett 2(2): 74–75

    Article  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1965) Nutrient requirements of suspension cultures of soybean root callus. Exp Cell Res 50:151–158

    Article  Google Scholar 

  • Glaszmann JC (1987) Isozymes and classification of Asian rice varieties. Theor Appl Genet 74:21–30

    Article  CAS  Google Scholar 

  • Ham C (1973) Fusion of protoplasts isolated from rice callus. SABRAO Newslett 5:107–110

    Google Scholar 

  • Hayashi Y, Kyozuka J, Shimamoto K (1988) Hybrids of rice (Oryza saliva L.) and wild Oryza species obtained by cell fusion. Mol Gen Genet 214:6–10

    Article  Google Scholar 

  • International Rice Research Institute (IRRI) (ed) (1985) Biotechnology in international agricultural research. IRRI, Manila, Philipp

    Google Scholar 

  • Kao KN (1977) Chromosomal behaviour in somatic hybrids of soybean Nicotiana glauca. Mol Gen Genet 150:225–230

    Article  Google Scholar 

  • Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110

    Article  CAS  Google Scholar 

  • Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206:408–413

    Article  CAS  Google Scholar 

  • Kyozuka J, Kaneda T, Shimamoto K (1989) Production of cytoplasmic male sterile rice (Oryza saliva L.) by cell fusion. Biotechnology 7:1171–1174

    Google Scholar 

  • Kyozuka J, Otoo E, Shimamoto K (1988) Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet 76:887–890

    Article  Google Scholar 

  • Lai K-I, Liu L-F (1976) On the isolation and fusion of rice protoplasts. J Agric Assoc China, N Ser 93:10–29

    Google Scholar 

  • Lee CH, Power JB (1988) Intraspecific gametosomatic hybridization in Petunia hybrida. Plant Cell Rep 7:17–18

    Article  Google Scholar 

  • Lee L, Schroll RE, Grimes HD, Hodges TK (1989) Plant regeneration from indica rice (Oryza sativa L.) protoplasts. Planta 178(3):325–333

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol Plant 18:100–127

    Article  CAS  Google Scholar 

  • Lörz H, Göbel E, Brown P (1988) Advances in tissue culture and progress towards genetic transformation of cereals. Plant Breed 100:1–25

    Article  Google Scholar 

  • Muller AG, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161:67–76

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Niizeki M, Kita F (1981) Cell division of rice and soybean and their fused protoplasts. Jpn J Breed 31, 2:161–167

    Google Scholar 

  • Niizeki M, Tanaka M, Akada S, Hirai A, Saito K (1985) Callus formation of somatic hybrid of rice and soybean and characteristics of the hybrid callus. Jpn J Genet 60:81–92

    Article  Google Scholar 

  • Ogura H, Kyozuka J, Hayashi Y, Koba T, Shimamoto K (1987) Field performance and cytology of protoplast-derived rice (Oryza sativa): high yield and low degree of variation of four japonica cultivars. Theor Appl Genet 74:670–676

    Article  Google Scholar 

  • Ohira JK, Ojima K, Fujimura A (1973) Studies on the nutrition of rice cell culture 1: a simple, defined medium for rapid growth in cell suspension. Plant Cell Physiol 14:113–121

    Google Scholar 

  • Pental D, Mukhopadhyay A, Grover A, Pradhan AK (1988) A selection method for the synthesis of triploid hybrids (3n) by fusion of microspore protoplasts (n) with somatic cell protoplasts (2n). Theor Appl Genet 76(2):237–243

    Google Scholar 

  • Pirrie A, Power JB (1986) The production of fertile, triploid somatic hybrid plants [Nicotiana glutinosa (n) + N. tabacum (2n)] via gametic: somatic protoplast fusion. Theor Appl Genet 72:48–52

    Article  Google Scholar 

  • Saleh NM, Gupta HS, Finch RP, Cocking EC, Mulligan BJ (1989) Stability of mitochondrial DNA in tissue cultured cells of rice. Theor Appl Genet (in press)

    Google Scholar 

  • Swaminathan MS (1986) Plant research and world agriculture. Plant Mol Biol Rep 4:1–17

    Article  Google Scholar 

  • Terada R, Kyozuka J, Nishibayashi S, Shimamoto K (1987) Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing). M of Gen Genet 210:39–43

    Article  Google Scholar 

  • Thompson JA, Abdullah R, Cocking EC (1986) Protoplast culture of rice (Oryza saliva L.) using media solidified with agarose. Plant Sci 47:123–133

    Article  Google Scholar 

  • Thompson JA, Abdullah R, Chen W-H, Gartland KMA (1987) Enhanced protoplast division in rice (Oryza saliva L.) following heat shock treatment. J Plant Physiol 127:367–370

    Google Scholar 

  • Toriyama K, Hinata K (1985) Panicle culture in liquid media for obtaining anther calli and protoplasts of rice. Jpn J Breed 35:449–452

    Google Scholar 

  • Toriyama K, Hinata K (1988) Diploid somatic-hybrid plants regenerated from rice cultivars. Theor Appl Genet 76:665–668

    Article  Google Scholar 

  • Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor App(Genet 73:16–19

    Google Scholar 

  • Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. Bio/technology 6:1072–1074

    Article  CAS  Google Scholar 

  • Uchimiya H, Fushimi T, Hashimoto H, Harada H, Syono K, Sugawawara Y (1986) Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza saliva L.). Mol Gen Genet 204:204–207

    Article  CAS  Google Scholar 

  • Watts JW, King J M (1984) A simple method for large-scale electrofusion and culture of plant protoplasts. Biosci Rep 4:335–342

    Article  PubMed  CAS  Google Scholar 

  • Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47:189–194

    PubMed  CAS  Google Scholar 

  • Yamada Y, Qi YZ, Tai TD (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 5:85–88

    Article  Google Scholar 

  • Yang H, Zhang HM, Davey MR, Mulligan BJ, Cocking EC (1988) Production of kanamycin-resistant rice tissue following DNA uptake into protoplasts. Plant Cell Rep 7:421–425

    CAS  Google Scholar 

  • Yang Z-Q, Shikanai T, Yamada Y (1988) Asymmetric hybridization between cytoplasmic male-sterile (CMS) and fertile rice (Oryza sativa L.) protoplasts. Theor Appl Genet 76:801–808

    Article  Google Scholar 

  • Zhang HM, Yang H, Rech EL, Golds TJ, Davis AS, Mulligan BJ, Cocking EC, Davey MR (1988) Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts. Plant Cell Rep 7:379–384

    CAS  Google Scholar 

  • Zhang W, Wu R (1988) Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants. Theor Appl Genet 76:835–840

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Finch, R.P., Lynch, P.T., Jotham, J.P., Cocking, E.C. (1991). Isolation, Culture, and Fusion of Rice Protoplasts. In: Bajaj, Y.P.S. (eds) Rice. Biotechnology in Agriculture and Forestry, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83986-3_17

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-83986-3_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83988-7

  • Online ISBN: 978-3-642-83986-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics