Rice pp 193-215 | Cite as

Factors Affecting Androgenesis in Rice (Oryza sativa L.)

  • Chi-Chang Chen
  • Hsin-Sheng Tsay
  • Chien-Rong Huang
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 14)

Abstract

The frequency of the induction of androgenesis in cultured anthers is in general rather low. In order to utilize anther culture in crop breeding programs, it is essential that the haploids are produced in large numbers. In this chapter various factors responsible for enhancing androgenesis in rice are discussed.

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References

  1. Anagnostakis SL (1974) Haploid plants from anthers of tobacco enhancement with charcoal. Planta 115: 281–283CrossRefGoogle Scholar
  2. Anon (1976) A sharp increase of the frequency of pollen plant induction in wheat with potato medium. Acta Genet Sin 3: 25–31Google Scholar
  3. Bajaj YPS (1977) In vitro induction of haploids in wheat (Triticum aestivum L.). Crop Improv 4: 54–64Google Scholar
  4. Bajaj YPS (1983) In vitro production of haploids. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol I. Techniques for propagation and breeding. MacMillan Press, New York, pp 228–287Google Scholar
  5. Bajaj YPS (ed) (1990) Biotechnology in agriculture and forestry, vol 12: Haploids in crop improvement I. Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  6. Bajaj YPS, Reinert J, Heberle E (1977) Factors enhancing in vitro production of haploid plants in anthers and isolated microspores. In: Gautheret R (ed) La culture des tissus et des cellules des végétaux. Mason, Paris, pp 47–58Google Scholar
  7. Bernard S (1980) In vitro androgenesis in hexaploid triticale: determination of physical conditions increasing embryoid and green plant production. Z Pflanzenzucht 85: 308–321Google Scholar
  8. Chaleff RS, Stolarz A (1981) Factors influencing the frequency of callus formation among cultured rice (Orvza saliva) anthers. Physiol Plant 51: 201–206CrossRefGoogle Scholar
  9. Chen CC (1976) Studies on the anther culture of rice: pollen stage and low-temperature treatment. Natl Sci Counc M Taipei 4: 2187–2190Google Scholar
  10. Chen CC (1977) In vitro development of plants from microspores of rice. In Vitro 13: 484–489PubMedCrossRefGoogle Scholar
  11. Chen CC (1978) Effects of sucrose concentration on plant production in anther culture of rice. Crop Sci 18: 905–906CrossRefGoogle Scholar
  12. Chen CC, Chen CM (1979) A method for anther culture of rice. Tissue Cult Assoc Manual 5: 1051–1053CrossRefGoogle Scholar
  13. Chen CC, Chen CM (1980) Changes in chromosome number in microspore callus of rice during successive subculture. Can J Genet Cytol 22: 607–614Google Scholar
  14. Chen CC, Lin CM (1981) Genotypic differences in plant production in anther culture of rice. In: Chang WC (ed) Proc Symp Plant cell tissue culture. Acad Sin, Taipei, pp 199–203Google Scholar
  15. Chen CC, Lin MH (1976) Induction of rice plantlets from anther culture. Bot Bull Acad Sin 17: 18–24Google Scholar
  16. Chen CC, Wu YH (1983) Segmentations in microspores of rice during anther culture. Proc Natl Sci Counc B 7: 151–157Google Scholar
  17. Chen CC, Chiu WL, Yu LJ, Ren SS, Yu WJ, Lin MH (1983) Genetic analysis of anther-derived plants of rice: tests for independent assortment of unlinked genes. Can J Genet Cytol 25: 324–328Google Scholar
  18. Chen CM, Chen CC, Lin MH (1982) Genetic analysis of anther-derived plants of rice. J Hered 73: 49–52Google Scholar
  19. Chen JJ, Tsay HS (1984) The callus-forming ability of rice anther originated from tillers ofdifferent order and from spikelets ofdifferent positions and branches on the panicles. J Agric Res China 33,4: 354–362Google Scholar
  20. Chen JJ, Tsay HS (1986) Effects of ginseng on rice anther culture. J Agric Res China 35,2: 139–144Google Scholar
  21. Chen JJ, Chen U. Tsay HS (1984) Relationship between the time for callus initiation, callus age and the ability of organ regeneration from rice anther culture. J Agric Assoc China 126: 2–9Google Scholar
  22. Chen JJ, Hsu JY, Tsay HS (1986) Effect of iron on rice anther culture. J Agric Res China 35,3: 244–252Google Scholar
  23. Chen LJ, Lai PC, Liao CH, Tsay HS (1982) Medium evaluation for rice anther culture. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 551–552Google Scholar
  24. Chen Y, Tso CH, Wang JF, Chang KH (1978) On screening of anther culture media for hybrid Oryza sativa L. subsp. Keng x O. saliva subsp. Shien by orthogonal test. In: Hu H (ed) Proc Symp Anther culture. Science Press, Beijing, pp 40–49Google Scholar
  25. Chen Y, Zuo QZ, Li SY, Qu RD (1982) Plant regeneration from isolated rice pollen culture and some factors affecting induction frequency. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 559–560Google Scholar
  26. Chen ZH (1982) Anther culture techniques of rubber tree and sugarcane. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 533–534Google Scholar
  27. Chu CC (1978) The N, medium and its applications to anther culture of cereal crops. In: Proc Symp Plant tissue culture. Science Press, Beijing, pp 45–50Google Scholar
  28. Chu CC (1982) Haploids in plant improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York London, pp 129–158Google Scholar
  29. Chu CC, Wang CS, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 17: 657–668Google Scholar
  30. Chuang CC, Ouyang TW, Chia H. Chou SM, Ching CK (1978) A set of potato media for wheat anther culture. In: Proc Symp Plant tissue culture. Science Press, Beijing, pp 51–56Google Scholar
  31. Clapham D (1973) Haploid Hordeum plants from anthers in vitro. Z Pflanzenzucht 69: 142–155Google Scholar
  32. Clapham D (1977) Haploid induction in cereals. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 279–298Google Scholar
  33. Collins GB (1977) Production and utilization of anther-derived haploids in crop plants. Crop Sci 17: 583–586CrossRefGoogle Scholar
  34. Cornejo-Martin MJ, Primo-Millo E (1981) Anther and pollen grain culture of rice (Oryza saliva L.). Euphytica 30: 541–546CrossRefGoogle Scholar
  35. De Buyser J, Henry Y (1980) Comparison of different media used in culturing anthers in vitro in soft wheat. Can J Bot 58: 997–1000CrossRefGoogle Scholar
  36. Dunwell JM (1976) A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environ Exp Bot 16: 109–118CrossRefGoogle Scholar
  37. Dunwell JM (1979) Anther culture in Nicotiana tabacum: the role of the culture vessel atmosphere in pollen embryo induction and growth. J Exp Bot 30: 419–428CrossRefGoogle Scholar
  38. Dunwell JM (1981) Stimulation of pollen embryo induction in tobacco by pretreatment of excised anthers in a water-saturated atmosphere. Plant Sci Lett 21: 9–13CrossRefGoogle Scholar
  39. Foroughi-Wehr B, Friedt W. Wengel G (1982) On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor Appl Genet 62: 233–239Google Scholar
  40. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158PubMedCrossRefGoogle Scholar
  41. Genovesi AD, Collins GB (1982) In vitro production of haploid plants of corn via anther culture. Crop Sci 22: 1137–1144CrossRefGoogle Scholar
  42. Genovesi AD, Magill CW (1979) Improved rate of callus and green plant production from rice anther culture following cold shock. Crop Sci 19: 662–664CrossRefGoogle Scholar
  43. Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107: 161–170CrossRefGoogle Scholar
  44. Gresshoff PM, Doy CH (1974) Derivation of a haploid cell line from Vitis vinifiera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z Pflanzenphysiol 73: 132–141Google Scholar
  45. Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature (London) 204: 497CrossRefGoogle Scholar
  46. Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in pollen grains of Datura in vitro. Nature (London) 212: 97–98CrossRefGoogle Scholar
  47. Guha S, Iyer RD, Gupta N, Swaminathan MS (1970) Totipotency of gametic cells and the production of haploids in rice. Curr Sci 39: 174–176Google Scholar
  48. Guha-Mukherjee S (1973) Genotypic differences in the in vitro formation of embryoids from rice pollen. J Exp Bot 24: 139–144CrossRefGoogle Scholar
  49. Harn C (1969) Studies on anther culture of rice. Korean J Breed 1: 1–11Google Scholar
  50. Horner M, Mott RL (1979) The frequencyofembryogenic pollen grains is not increased by in vitro anther culture in Nicotiana tabacum L. Planta 147: 156–158CrossRefGoogle Scholar
  51. Homer M, Pratt ML (1979) Amino acid analysis of in vivo and androgenic anthers of Nicotiana tabacum. Protoplasma 98: 279–282CrossRefGoogle Scholar
  52. Homer M, Street HE (1978) Pollen dimorphism origin and significance in pollen plant formation by anther culture. Ann Bot (London) 42: 763–771Google Scholar
  53. Hsu SC, Chen CC (1977) Studies on the anther culture of rice: origin of the anther-derived diploid plants. Natl Sci Counc M 5: 834–839Google Scholar
  54. Hu C, Huang SC, Ho CP, Liang HC, Chuang CC, Peng LP (1978) On the inductive conditions of rice pollen plantlets in anther culture. In: Proc Symp Plant tissue culture. Science Press, Beijing, pp 87–95Google Scholar
  55. Huang B, Sunderland N (1980/1981) Stress pretreatment in barley anther culture. John Innes Inst Annu Rep No 71: 63–64Google Scholar
  56. Huang CR, Wu YH, Chen CC (1985) Effects of plant growth substances on callus formation and plant regeneration in anther culture of rice. In: Khush GS (ed) Proc Int Rice Genet Symp IRRI, Los Banos, pp 763–771Google Scholar
  57. Huang HS, Lin TH, Tseng PL, Hsian YL, Shi P, Ho HL, Pang CC, Wang HY. Huang PT, Tseng JF (1978) Studies on composition of culture medium of Oryza saliva L. subspecies Shien by methods of mathematical analysis. In: Hu H (ed) Proc Symp Anther culture. Science Press, Beijing, pp 29–39Google Scholar
  58. Inoue M, Maeda E (1981) Stimulation of shoot bud and plantlet formation in rice callus cultures by two-step culture method using abscisic acid and kinetin. Jpn J Crop Sci 50: 318–322CrossRefGoogle Scholar
  59. Lrikura Y (1975) Induction of haploid plants by anther culture in tuber-bearing species and interspecific hybrids of Solanum. Potato Res 18: 133–140CrossRefGoogle Scholar
  60. Iyer RD, Raina SK (1972) The early ontogeny of embryoids and callus from pollen and subsequent organogenesis in anther cultures of Datura metel and rice. Planta 104: 146–156CrossRefGoogle Scholar
  61. Jacobsen E, Sopory SK (1978) The influence of possible recombination of genotypes on the production of microspore embryoids in anther cultures of Solanum tuberosum and dihaploid hybrids. Theor Appl Genet 52: 119–123CrossRefGoogle Scholar
  62. Johansson L, Andersson B, Eriksson T (1982) Improvement of anther culture technique: activated charcoal bound in agar medium in combination with liquid medium and elevated CO., concentration. Physiol Plant 54: 24–30CrossRefGoogle Scholar
  63. Keller WA, Armstrong KC (1978) High frequency production of microspore-derived plants from Brassica napus anther cultures. Z Pflanzenzucht 80: 100–108Google Scholar
  64. Keller WA, Armstrong KC (1979) Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments. Theor Appl Genet 55: 65–67CrossRefGoogle Scholar
  65. Kohlenbach HW, Wernicke W (1978) Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z Pflanzenphysiol 86: 463–472Google Scholar
  66. Kohlenhach HW, Wernicke W, Lang H (1978) Androgenetic development of isolated microspores of Hyocyamus and Nicotiana. In: Alfermenn AW, Reinhard E (ed) Production of natural compounds by cell culture methods. Ges Strahlen Umweltforsch. Munich, pp 284–294Google Scholar
  67. Lee J, Chen CC (1982) Genetic and histological evidence for microspore origin of anther-derived plants of rice. Taiwania 27: 86–92Google Scholar
  68. Liang HM (1978) The advance of studies on medium for anther culture of rice in China. In: Proc Symp Plant Tissue Cult. Science Press, Beijing, pp 57–64Google Scholar
  69. Lin CI, Tzen MT, Tsay HS (1974) Some influencing factors affecting callus formation from in vitro cultured anthers of rice plants. Mem Collog Agric Natl Taiwan Univ 15: 1–16Google Scholar
  70. Lin MH (1979) Diploidization of haploid rice plants by colchicine treatment. J Agric Res China 28:45–49Google Scholar
  71. Lin SL, Tsay HS (1984) Effect of cold treatment on rice anther culture. J Agric Assoc China 127:8–17Google Scholar
  72. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol Plant 18: 100–127CrossRefGoogle Scholar
  73. Maheshwari SC, Tyagi AK, Malhotra K (1980) Induction of haploidy from pollen grains in angiosperms — the current status. Theor Appl Genet 58: 193–206CrossRefGoogle Scholar
  74. Malhotra K, Maheshwari SC (1977) Enhancement by cold treatment of pollen embryoid development in Petunia hybrida. Z Pflanzenphysiol 85: 177–180Google Scholar
  75. Miao SH, Kuo CS, Kwei YL, Sun AT, Ku SY, Lu WL, Wang YY (1978) Induction of pollen plants of maize and observations on their progeny. In: Proc Symp Plant tissue culture. Science Press, Beijing, pp 23–33Google Scholar
  76. Mii M (1976) Relationships between anther browning and plantlet formation in anther culture of Nicotiana tabacum L. Z Pflanzenphysiol 80: 206–214Google Scholar
  77. Miller CO (1963) Kinetin and kinetin-like compounds. In: Linskens HF, Tracey MV (eds) Moderne Methoden der Pflanzenanalyse. Springer, Berlin Heidelberg New York, pp 194–202Google Scholar
  78. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  79. Nakamura A, Itagaki R (1973) Anther culture in Nicotiana and the characterization of the haploid plants. Jpn J Breed 23: 71–78Google Scholar
  80. Niizeki H, Oono K (1968) Induction ofhaploid rice plant from anther culture. Proc Jpn Acad 44: 554–557Google Scholar
  81. Niizeki H, Oono K (1971) Rice plants obtained by anther culture. In: Les culture de tissue des plantes. Colloq Int CNRS Paris 193: 251–257Google Scholar
  82. Nishi T, Mitsuoka S (1969) Occurrence of various ploidy plants from anther and ovary culture of rice plant. Jpn J Genet 44: 341–346CrossRefGoogle Scholar
  83. Nitsch C (1974) Pollen culture — a new technique for mass production of haploid and homozygous plants. In: Kasha K (ed) Haploids in higher plants, advances and potential. Univ Press, Guelph, pp 123–135Google Scholar
  84. Nitsch C, Norreel B (1973) Effet d’un choc thermique sur le pouvoir embryogene du pollen de Datura innoxia cultive dans l’anthere ou isole de l’anthere. C R Acad Sci Paris Ser D 276: 303–306Google Scholar
  85. Nitsch C, Anderson S, Godard M, Neuffer MG, Sheridan WP (1982) Production ofhaploid plants of Zea mays and Pennisetum through androgenesis. In: Earle ED, Demarly Y (eds) Variability in plants regenerated from tissue culture. Praeger, New York, pp 69–91Google Scholar
  86. Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19: 389–404Google Scholar
  87. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains: Science 163: 85–87PubMedGoogle Scholar
  88. Ono H, Larter EN (1976) Anther culture of triticale. Crop Sci 16: 120–122CrossRefGoogle Scholar
  89. Oono K (1975) Production of haploid plants of rice (Oryza saliva) by anther culture and their use for breeding. Bull Natl Inst Agric Sci D 26: 139–222Google Scholar
  90. Ouyang JW, Hu H, Chuang CC, Tseng CC (1973) Induction of pollen plants from anthers of Triticum aestivum L. cultured in vitro. Sci Sin 16: 79–95Google Scholar
  91. Pelletier G, Ilami M (1972) Les facteurs de l’androgenese in vitro chez Nicotiana tabacum L. Z Pflanzenphysio l68: 97–114Google Scholar
  92. Rashid A, Reinert J (1980) Selection of embryogenic pollen from cold-treated buds of Nicotiana tabacum var. Badischer Burley and their development into embryos in cultures. Protoplasma 105: 161–167CrossRefGoogle Scholar
  93. Rashid A, Reinert J (1981) Differentiation of embryogenic pollen in cold-treated buds of Nicotiana tabacum var. Badischer Burley and nutritional requirements of the isolated pollen to form embryos. Protoplasma 106: 137–144CrossRefGoogle Scholar
  94. Reinert J, Bajaj YPS (1977) Anther culture: Haploid production and its significance. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 251–278Google Scholar
  95. Sun CS, Wang CC, Chu CC (1974) The ultrastructure of plastids in the albino pollen plants of rice. Sci Sin 17: 793–802Google Scholar
  96. Sun CS, Wu SC, Wang CC, Chu CC (1979) The deficiency of soluble proteins and plastid ribosomal RNA in the albino pollen plantlets of rice. Theor Appl Genet 55: 193–197CrossRefGoogle Scholar
  97. Sunderland N (1978) Strategies in the improvement of yields in anther culture. In: Proc Symp Plant tissue culture. Science Press, Beijing, pp 65–86Google Scholar
  98. Sunderland N (1980) Anther and pollen culture. 1974–1979. In: Davies DR, Hopwood DA (eds) The plant genome. Innes Charity, Norwich, pp 171–183Google Scholar
  99. Sunderland N, Dunwell JM (1977) Anther and pollen culture. In: Street HE (eds) Plant tissue and cell culture. Blackwell, Oxford, pp 223–265Google Scholar
  100. Sunderland N, Evans LJ (1980) Multicellular pollen formation in cultured barley anthers. II. The A. B, and C pathways. J Exp Bot 31: 501–514CrossRefGoogle Scholar
  101. Sunderland N, Roberts M (1977) New approach to pollen culture. Nature (London) 270: 236–238CrossRefGoogle Scholar
  102. Sunderland N, Roberts M (1979) Cold-pretreatment of excised flower buds in float culture of tobacco anthers. Ann Bot (London) 43: 405–414Google Scholar
  103. Sunderland N. Wicks FM (1971) Embryoid formation in pollen grains of Nicotiana tabacum. J Exp Bot 22: 213–226CrossRefGoogle Scholar
  104. Sunderland N, Wildon DC (1979) A note on the pretreatment of excised flower buds in float culture of Hyoscyamus anthers. Plant Sci Lett 15: 169–175CrossRefGoogle Scholar
  105. Sunderland N, Xu ZH, Huang B (1981) Recent advances in barley anther culture. In: Proc 4th Int Barley genetics Symp. Univ Press, Edinburgh, pp 699–703Google Scholar
  106. Tomes DT, Collins GB (1976) Factors affecting haploid plant production from in vitro anther culture of Nicotiana species. Crop Sci 16: 837–840CrossRefGoogle Scholar
  107. Torrizo LB, Zapata FJ (1986) Anther culture in rice: IV. The effect of abscisic acid on plant regeneration. Plant Cell Rep 5: 136–139CrossRefGoogle Scholar
  108. Tsai SC, Lin MH (1977) Production of rice plantlets by anther culture. J Agric Res China 26: 100–112Google Scholar
  109. Tsay HS (1981) Effects of nitrogen supply to donor plants on pollen embryogenesis in cultured tobacco anthers. J Agric Res China 30: 5–13Google Scholar
  110. Tsay HS (1982) Autotoxicity in tobacco and rice anther culture. In: Chou CH, Waller GR (eds) Allelochemicals and pheromones. Acad Sin, Taipei, Taiwan, ROC, pp 283–292Google Scholar
  111. Tsay HS, Chen LJ (1984) The effects of cold shock and liquid medium on callus formation in rice anther culture. J Agric Res China 33,1: 24–29Google Scholar
  112. Tsay HS, Teng YC, Lai PC, Chi NC (1981) The culture of rice anthers ofjaponica X indica crosses. J Agric Res China 30: 133–139Google Scholar
  113. Tsay HS, Chen LJ, Tseng TH, Lai PC (1982) The culture of rice anthers ofjaponica X indica crosses. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 561–562Google Scholar
  114. Tsay HS, Chen JJ, Yeh CC, Hsu JY (1988) Influence of cold shock treatment and microspore developmental stage on rice anther culture. J Agric Res China 37,3: 257–265Google Scholar
  115. Tsay SS, Tsay HS, Chao CY (1986) Cytochemical studies of callus development from microspore in cultured anther of rice. Plant Cell Rep 5: 119–123CrossRefGoogle Scholar
  116. Tyagi AK, Rashid A, Maheshwari SC (1979) High frequency production of embryos in Datura innoxia from isolated pollen grains by combined cold treatment and serial culture of anthers in liquid medium. Protoplasma 99:1l-17CrossRefGoogle Scholar
  117. Vaughn KC, Bonte De LR, Wilson KG, Schaeffer GW (1980) Organelle alteration as a mechanism for maternal inheritance. Science 208: 196–198PubMedCrossRefGoogle Scholar
  118. Wang CC, Sun CS, Chu Z (1974) On the conditions for the induction of rice pollen plantlets and certain factors affecting the frequency of induction. Acta Bot Sin 16: 43–53Google Scholar
  119. Weatherhead MA, Burdon J. Henshaw GG (1978) Some effects of activated charcoal as an additive to plant tissue culture media. Z Pflanzenphysiol 89: 141–147Google Scholar
  120. Wenzel G, Hoffman F, Thomas E (1977) Increased induction and chromosome doubling of androgenetic haploid rye. Theor Appl Genet 51: 81–86CrossRefGoogle Scholar
  121. Wernicke W, Kohlenbach HW (1976) Investigation on liquid culture medium as a means of anther culture in Nicotiana. Z Pflanzenphysiol 79: 189–198Google Scholar
  122. Wilson HM, Mix G, Foroughi-Wehr B (1978) Early microspore divisions and subsequent formation of microspore calluses at high frequency in anthers of Hordeum vulgare L. J Exp Bot 29: 227–238CrossRefGoogle Scholar
  123. Woo SC, Huang CY (1982) Anther culture of Oryza sativa L. X Oryza spontaneous taiwania hybrids. Bot Bull Acad Sin 23: 39–44Google Scholar
  124. Woo SC, Tung IJ (1972) Induction of rice plants from hybrid anthers of indica and japonica cross. Bot Bull Acad Sin 13: 67–70Google Scholar
  125. Woo SC, Mok T, Huang JY (1978) Anther culture of Oryza saliva L. and Oryza perennis M oench hybrids. Bot Bull Acad Sin 19: 171–178Google Scholar
  126. Xu ZH, Sunderland N (1981) Glutamine, inositol and conditioning factor in the production of barley pollen callus in vitro. Plant Sci Lett 23: 161–168CrossRefGoogle Scholar
  127. Xu ZH, Huang B, Sunderland N (1981) Culture of barley anthers in conditioned media. J Exp Bot 32: 767–778CrossRefGoogle Scholar
  128. Yeh CC, Tsay HS (1987) Effect of inoculation time during the year on the culture efficiency of rice anther. J Agric Res China 36,3: 247–253Google Scholar
  129. Yeh CC, Tsay HS (1988) The effect of temperature treatment and medium composition on rice anther culture. J Agric Res China 37,3: 250–256Google Scholar
  130. Zapata FJ, Torrizo LB, Ramero RO, Alejar MS (1982) Androgenesis in Oryza saliva. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 531–532Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Chi-Chang Chen
    • 1
  • Hsin-Sheng Tsay
    • 2
  • Chien-Rong Huang
    • 1
  1. 1.Department of BotanyNational Taiwan UniversityTaipeiTaiwan, China
  2. 2.Department of AgronomyTaiwan Agricultural Research InstituteTaichung, TaipeiTaiwan, China

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