Intestinal Calcium Transport and Bone Mineralization in the Spontaneously Diabetic BB Rat

  • R. Bouillon
  • J. Verhaeghe
  • M. Thomasset
Conference paper
Part of the NATO ASI Series book series (volume 48)

Abstract

Diabetes mellitus is associated with a large number of abnormalities in calcium homeostasis: urinary calcium excretion in both human and experimental diabetes is increased; the duodenal calcium absorption is usually decreased in streptozotocin-induced diabetic rats and the duodenal calbindin concentration is similarly decreased (Table 1). The osteoblast function also seems to be impaired as suggested by decreased bone formation and possibly decreased bone mass in both experimental and human insulindependent diabetes mellitus (1). Moreover, the fetus or neonate of diabetic mothers have an increased risk for bone and calcium abnormalities, such as the caudal regression syndrome and neonatal hypocalcemia. The pathogenesis of these abnormalities is not well known. We, therefore, used the spontaneously diabetic BB rat, a model of human autoimmune diabetes, to study the effect of insulin deficiency on vitamin D and calcium homeostasis.

Keywords

Phosphorus Filtration Lactate Osteoporosis Hyperglycemia 

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References

  1. 1.
    Auwerx J, Dequeker J, Bouillon R, Geusens P, Nijs J (1988) Mineral metabolism and bone mass at peripheral and axial skeleton in diabetes mellitus. Diabetes 37: 8–12PubMedCrossRefGoogle Scholar
  2. 2.
    Bouillon R, De Moor P, Baggiolini EG, Uskokovic MR (1980) A radioimmunoassay for 1,25-dihydroxycholecalciferol. Clin Chem 26: 562–567PubMedGoogle Scholar
  3. 3.
    Charles MA, Tirunaguru P, Zolock DT, Morissey RL (1981) Duodenal calcium transport and calcium binding protein levels in experimental diabetes mellitus. Miner Electrolyte Metab 5: 15–22Google Scholar
  4. 4.
    Hough S, Avioli AV (1984) Alterations of bone and mineral metabolism in diabetes. In: Recent advances in diabetes, pp. 223–229. Nattrass M, Santiago JV (eds). Edinburgh: Churchill LivingstoneGoogle Scholar
  5. 5.
    Like AA (1985) Spontaneous diabetes in animals. In: Volk B, Arquilla ER. The diabetic pancreas. 2nd ed.: 385–413Google Scholar
  6. 6.
    Nyomba BL, Verhaeghe J, Thomasset M, Lissens W, Bouillon R (1989) Bone mineral homeostasis in spontaneously diabetic BB rats. I. Abnormal vitamin D metabolism and impaired active intestinal calcium absorption. Endocrinology 124: 565–572PubMedCrossRefGoogle Scholar
  7. 7.
    Pansu D, Bellaton C, Roche C, Bronner F (1983) Duodenal and ileal calcium absorption in the rat and effects of vitamin D. Am J Physiol 244: G659 - G700Google Scholar
  8. 8.
    Schedl HP, Heath H, Wenger J (1978) Serum calcitonin and parathyroid hormone in experimental diabetes: effects of insulin treatment. Endocrinology 103: 1368–1373PubMedCrossRefGoogle Scholar
  9. 9.
    Schneider LE, Schedl HP (1972) Diabetes and intestinal calcium absorption in the rat. Am J Physiol 223: 1319–1323PubMedGoogle Scholar
  10. 10.
    Schneider LE, Wilson HD, Schedi HP (1974) Effects of alloxan diabetes on duodenal calcium-binding protein in the rat. Am J Physiol 227: 832–838PubMedGoogle Scholar
  11. 11.
    Schneider LE, Omdahl J, Schedl HP (1976) Effects of vitamin D and its metabolites on calcium transport in the diabetic rat. Endocrinology 99: 793–799PubMedCrossRefGoogle Scholar
  12. 12.
    Thomasset M, Parkes CO, Cuisinier-Gleizes P (1982) Rat calcium-binding proteins: distribution, development, and vitamin D dependence. Am J Physiol 243: E483 - E488PubMedGoogle Scholar
  13. 13.
    Verhaeghe J (1988) Calcium metabolism during reproduction in the diabetic BB rat. Thesis K.U. Leuven, pp. 1–154Google Scholar
  14. 14.
    Verhaeghe J, Thomasset M, Bréhier A, Van Assche FA, Bouillon R (1988) 1,25(OH)2D3 and Ca-binding protein in fetal rats: relationship to the ‘maternal vitamin D status. Am J Physiol 254: E505 - E512Google Scholar
  15. 15.
    Verhaeghe J, Suiker AMH, Nyomba BL, Visser WJ, Einhorn TA, Dequeker J, Bouillon R (1989) Bone mineral homeostasis in spontaneously diabetic BB rats. II. Impaired bone turnover and decreased osteocalcin synthesis. Endocrinology 124: 573–582PubMedCrossRefGoogle Scholar
  16. 16.
    Verhaeghe J, Van Herck E, Van Bree R, Van Assche FA, Bouillon R (1989) Osteocalcin during the reproductive period in normal and diabetic rats. J Endocr 120: 143–151PubMedCrossRefGoogle Scholar
  17. 16.
    Verhaeghe J, Van Herck E, Van Bree R, Van Assche FA, Bouillon R (1989) Osteocalcin during the reproductive period in normal and diabetic rats. J Endocr 120: 143–151PubMedCrossRefGoogle Scholar
  18. 18.
    Wood RI, Allen LH, Bronner F (1984) Regulation of calcium metabolism in streptozotocininduced diabetes. Am J Physiol 247: R120 - R123PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • R. Bouillon
    • 1
  • J. Verhaeghe
    • 1
  • M. Thomasset
    • 2
  1. 1.Laboratorium voor Experimentele Geneeskunde en EndocrinologieOnderwijs & NavorsingLeuvenBelgium
  2. 2.Inserm U120Le VésinetFrance

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