cAMP-Dependent Protein Kinase: Subunit Diversity and Functional Role in Gene Expression

  • Matthias Meinecke
  • Wolfgang Büchler
  • Lilo Fischer
  • Suzanne M. Lohmann
  • Ulrich Walter
Conference paper
Part of the NATO ASI Series book series (volume 43)

Abstract

The cAMP-dependent protein kinase (cAMP-PK) exists as an inactive tetramer of two regulatory (R) and two catalytic (C) subunits which are dissociated by cAMP to form an R dimer and two C monomers. The free C subunits are active for substrate phosphorylation. Several subunits have been purified and structurally defined by amino acid sequencing. More recently, heterogenous forms which appear to be products of different genes have been identified by cDNA sequencing. So far a greater number of R subunits than C subunits have been found. The spectrum of mammalian subunits is shown in Table I. From the four different R subunits and two different C subunits, at least 8 different tetrameric holoenzymes can be formed. This minimal number assumes only homodimers of identical R or C subunits. Heterodimers of types I and II R subunits are not found in a holoenzyme complex, however, it has not been demonstrated whether or not α and ß forms can combine to make heterodimers of R-I or R-II.

Keywords

Adenosine Pyruvate Prolactin Plasminogen Chloramphenicol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Matthias Meinecke
    • 1
  • Wolfgang Büchler
    • 1
  • Lilo Fischer
    • 1
  • Suzanne M. Lohmann
    • 1
  • Ulrich Walter
    • 1
  1. 1.Department of Internal Medicine, Laboratory of Clinical BiochemistryUniversity of WürzburgWürzburgWest Germany

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