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Ultrastructure and AG Staining of Echinoderm Spermatogenesis

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Part of the book series: NATO ASI Series ((ASIH,volume 45))

Abstract

During the past 20 years, only a few fine structural observations have been made on the entire spermatogenetic process of echinoderms. In this paper we review the process of acrosome formation in the Echinodermata with emphasis on those aspects which still remain obscure. Silver nitrate staining at the ultrastructural level is also discussed and its application to the study of echinoderm spermatogenesis is presented.

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References

  • Afzelius BA (1977) Spermatozoa and spermatids of the crinoid Antedon petasus, with note on primitive spermatozoa from Deuterostome animals. J Ultrastruct Res 59: 272–281

    Article  PubMed  CAS  Google Scholar 

  • Anderson WA (1968) Cytochemistry of sea urchin gametes. III. Acid and alkaline Phosphatase activity of spermatozoa and fertilization. J Ultrastruct Res 25: 1–14

    Article  PubMed  CAS  Google Scholar 

  • Atwood DG (1974) Fine structure of spermatogonia, spermatocytes, and spermatids of the sea cucumbers Cucumaria lubrica and Leptosynapta clarki (Echinodermata:Holothuroidea). Can J Zool 52: 1389–1396

    Article  PubMed  CAS  Google Scholar 

  • Bickell LR, Chia F-S, Crawford BJ (1980) A fine structural study of the testicular wall and spermatogenesis in the crinoid, Florometra serratissima (Echinodermata). J Morphol 166: 109–126

    Article  Google Scholar 

  • Boldt J, Alliegro MC, Schuel H (1984) A separate catalase and peroxidase in sea urchin sperm. Gamete Res 10: 267–281

    Article  CAS  Google Scholar 

  • Buckland-Nicks J, Walker CW, Chia F-S (1984) Ultrastructure of the male reproductive system and of spermatogenesis in the viviparous brittle-star, Amphipholis squamata. J Morphol 179: 243–262

    Article  Google Scholar 

  • Conway AF, Metz CB (1976) Phospholipase activity of sea urchin sperm: its possible involvement in membrane fusion. J Exp Zool 198: 39–48

    Article  PubMed  CAS  Google Scholar 

  • Dan JC, Sirakami A (1971) Studies on the acrosome. X. Differentiation of the starfish acrosome. Dev Growth Differ 13: 37–52

    Article  PubMed  CAS  Google Scholar 

  • Erard MS, Belenguer P, Caizergues-Ferrer M, Pantaloni A, Amalric F (1988) A major nucleolar protein, nucleolin, induces chromatin decondensation by binding to histone H1. Eur J Biochem 175: 525–530

    Article  PubMed  CAS  Google Scholar 

  • Garbers DL (1989) Molecular basis of signalling in the spermatozoon. J Androl 10: 99–107

    PubMed  CAS  Google Scholar 

  • Green JD, Summers RG (1980) Ultrastructural demonstration of trypsin-like protease in acrosomes of sea urchin sperm. Science 209: 398–400

    Article  PubMed  CAS  Google Scholar 

  • Hoshi M, Moriya T (1980) Arylsulfatase of sea urchin sperm. 2. Arylsulfatase as a lysin of sea urchins. Dev Biol 74: 343–350

    Article  PubMed  CAS  Google Scholar 

  • Houk MS, Hinegardner RT (1981) Cytoplasmic inclusions specific to the sea urchin germ line. Dev Biol 86: 94–99

    Article  PubMed  CAS  Google Scholar 

  • Hylander BL, Summers RG (1975) An ultrastructural investigation of the spermatozoa of two ophiuroids, Ophiocoma echinata and Ophiocoma wendti: acrosomal morphology and reaction. Cell Tiss Res 158: 151–168

    Article  CAS  Google Scholar 

  • Janssen HH (1984) Development and ultrastructure of spermatozoa of Archaster typicus Mull. and Trosch. (Echinodermata, Asteroidea). Int J Invert Reprod Dev 7: 333–344

    Google Scholar 

  • Levine AE, Walsh KA (1979) Involvement of an acrosin-like enzyme in the acrosome reaction of sea urchin sperm. Dev Biol 72: 126–137

    Article  PubMed  CAS  Google Scholar 

  • Longo FJ, Anderson E (1969) Sperm differentiation in the sea urchins Arbacia punctulata and Strongylocentrotus purpuratus. J Ultrastruct Res 27: 486–509

    Article  PubMed  CAS  Google Scholar 

  • Longo FJ, Canvin AT, Bailey JL (1989) Membrane specializations associated with the acrosome complex of sea urchin sperm as revealed by immunocytochemistry and freeze fracture replication. Gamete Res 23: 429–440

    Article  PubMed  CAS  Google Scholar 

  • Mabuchi Y, Mabuchi I (1973) Acrosomal ATPase in starfish and bivalve mollusk spermatozoa. Exp Cell Res 82: 271–279

    Article  PubMed  CAS  Google Scholar 

  • Maekawa S, Sakai H (1982) Inhibitor protein of actin polymerization from starfish sperm head: purification and localization. Biomed Res 3: 46–53

    CAS  Google Scholar 

  • Maekawa S, Endo S, Sakai H (1982) A protein in starfish sperm head which bundles actin filaments in vitro: purification and characterization. J Biochem 92: 1959–1972

    PubMed  CAS  Google Scholar 

  • Moy GW, Vacquier VD (1979) Immunoperoxidase localization of bindin during the adhesion of sperm to sea urchin eggs. Curr Topics Dev Biol 13: 31–44

    Article  CAS  Google Scholar 

  • Nicotra A, Arizzi M, Gallo PV (1984) Protein synthetic activities during spermiogenesis in the sea urchin: an high resolution autoradiographic study of H-leucine incorporation. Dev Growth Differ 26: 273–280

    Article  CAS  Google Scholar 

  • Pladellorens M, Subirana JA (1975) Spermiogenesis in the sea cucumber Holothuria tubulosa. J Ultrastruct Res 52: 235–242

    Article  PubMed  CAS  Google Scholar 

  • Sardet C, Tilney LG (1977) Origin of the membrane for the acrosomal process: is actin complexed with membrane precursors? Cell Biol Int Rep 1: 193–200

    Article  PubMed  CAS  Google Scholar 

  • Sousa M, Azevedo C (1985) Acrosomal reaction and early events at fertilization in Marthasterias glacialis (Echinodermata:Asteroidea). Gamete Res 11: 157–167

    Article  Google Scholar 

  • Sousa M, Azevedo C (1986) Cytochemical study on the spermatozoon and at early fertilization in Marthasterias glacialis (Echinodermata, Asteroidea). Biol Cell 56: 79–84

    CAS  Google Scholar 

  • Sousa M, Azevedo C (1988a) Fine structural study of the acrosome formation in the starfish Marthasterias glacialis (Echinodermata, Asteroidea). Tiss Cell 20: 621–628

    Article  CAS  Google Scholar 

  • Sousa M, Azevedo C (1988b) Ultrastructure and silver-staining analysis of spermatogenesis in the sea urchin Paracentrotus lividus (Echinodermata, Echinoidea). J Morphol 195: 177–188

    Article  Google Scholar 

  • Sousa M, Azevedo C (1988c) Comparative silver staining analysis on spermatozoa of various invertebrate species. Int J Invert Reprod Dev 13: 1–8

    Google Scholar 

  • Sousa M, Azevedo C (1988d) Starfish acrosomal acid Phosphatase: a cytochemical and biochemical study. Biol Cell 63: 101–104

    Article  PubMed  CAS  Google Scholar 

  • Sousa M, Azevedo C (1988e) Presence of ATPase and alkaline Phosphatase activities in the starfish sperm acrosome. Cell Biol Int Rep 12: 1049–1054

    Article  PubMed  CAS  Google Scholar 

  • Sousa M, Azevedo C (1989a) Ultrastructural localization of calcium in the acrosome and jelly coat of starfish gametes. Dev Growth Differ 31: 227–232

    Article  Google Scholar 

  • Sousa M, Azevedo C (1989b) Silver staining of spermatogenesis in the starfish Marthasterias glacialis. Invert Reprod Dev 15: 105–108

    Article  Google Scholar 

  • Summers RG, Hylander BL (1974) An ultrastructural analysis of early fertilization in the sand dollar, Echinarachnius parma. Cell Tiss Res 150: 343–368

    Article  CAS  Google Scholar 

  • Tilney LG, Hatano S, Ishikawa H, Mooseker MS (1973) The polymerization of actin: role in the generation of the acrosomal process of certain echinoderm sperm. J Cell Biol 59:109–126

    Article  PubMed  CAS  Google Scholar 

  • Tilney LG (1976a) The polymerization of actin. II. How non filamentous actin becomes nonrandomly distributed in sperm: evidence for the association of this actin with membranes. J Cell Biol 69: 51–72

    Article  PubMed  CAS  Google Scholar 

  • Tilney LG (1976b) The polymerization of actin. III. Aggregates of nonfilamentous actin and its associated proteins: a storage form of actin. J Cell Biol 69: 73–89

    Article  PubMed  CAS  Google Scholar 

  • Tilney LG (1978) Polymerization of actin. V. A new organelle, the actomere, that initiates the assembly of actin filaments in Thyone sperm. J Cell Biol 77: 551–564

    Article  PubMed  CAS  Google Scholar 

  • Tilney LG, Inoué S (1982) Acrosomal reaction of Thyone sperm. II. The kinetics and possible mechanism of acrosomal process elongation. J Cell Biol 93: 820–827

    Article  PubMed  CAS  Google Scholar 

  • Vacquier VD, Moy GW (1977) Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs. Proc Natl Acad Sci USA 74: 2456–2460

    Article  PubMed  CAS  Google Scholar 

  • Yamada Y, Aketa K (1981) Vitelline layer lytic activity in sperm extracts of sea urchin, Hemicentrotus pulcherrimus. Gamete Res 4: 193–202

    Article  Google Scholar 

  • Yamagata A (1988) Ultrastructure of spermatogenesis in the sea star, Asterina minor. Gamete Res 19: 215–225

    Article  PubMed  CAS  Google Scholar 

  • Yamashita M (1983) A fine structural study of spermatogenesis in the brittle-star Ophiura sarsii (Echinodermata: Ophiuroidea), with a demonstration of the precocious formation of the acrosome. J Fac Sci Hokkaido Univ 23: 254–265

    Google Scholar 

  • Yamashita M, Iwata F (1983) Ultrastructural observations on the spermatogenesis of the brittle-star Amphipholis kochii Lutken (Echinodermata: Ophiuroidea). Seto Mar Biol Lab 5/6: 403–415

    Google Scholar 

  • Yanagimachi R (1986) The acrosome reaction: analysis of its mechanism using guinea pig spermatozoa. Dev Growth Differ 28: 3–4

    Article  Google Scholar 

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© 1990 Springer-Verlag Berlin Heidelberg

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Sousa, M., Azevedo, C. (1990). Ultrastructure and AG Staining of Echinoderm Spermatogenesis. In: Dale, B. (eds) Mechanism of Fertilization: Plants to Humans. NATO ASI Series, vol 45. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83965-8_9

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  • DOI: https://doi.org/10.1007/978-3-642-83965-8_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83967-2

  • Online ISBN: 978-3-642-83965-8

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