Skip to main content
SpringerLink
Log in

DNase Activity of 1,10-Phenanthroline-Copper Ion

  • Chapter
Nucleic Acids and Molecular Biology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 3))

Abstract

1,10-Phenanthroline-copper ion is an efficient chemical nuclease which makes single-stranded nicks in DNA at physiological pH’s and temperatures. In addition to serving as a reliable footprinting reagent, it is useful for defining functionally important sequence-dependent conformational variability of DNA and protein-induced structural changes in DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Cartwright IL, Elgin S (1982) Analysis of chromatin structure and DNA sequence organization: use of 1,10-phenanthroline-cuprous complex. NAR 10: 5835–5851

    Article  PubMed  CAS  Google Scholar 

  • Chen C-H B, Sigman DS (1986) Nuclease activity of 1,10-phenanthroline-copper. Sequence-specific targeting. Proc Natl Acad Sci USA 83: 7147–7151

    Article  PubMed  CAS  Google Scholar 

  • Chen C-H B. Sigman DS (1987) Chemical conversion of a DNA-binding protein into a site-specific nuclease. Science 237: 1197–1201

    Article  PubMed  CAS  Google Scholar 

  • Crothers DM (1987) Gel electrophoresis of protein-DNA complexes. Nature 325: 464–465

    Article  Google Scholar 

  • D’Aurora V, Stem AM, Sigman DS (1977) Inhibition of E. coliDNA polymerase I by 1,10-phenanthroline. Biochem Biophys Res Commun 78: 170–176

    Article  PubMed  Google Scholar 

  • D’Aurora V, Stern AM, Sigman DS (1978) 1,10-Phenanthroline-cuprous ion complex, a potent inhibitor of DNA and RNA polymerases. Biochem Biophys Res Commun 80:1025–1032

    Article  PubMed  Google Scholar 

  • de Crombnigghe B, Busby S, Buc H. (1984) Cyclic AMP receptor protein: role in transcription activation. Science 224: 831–837

    Article  Google Scholar 

  • Drew HR, Travers AA (1984) DNA structural variations in the E. colityr T promoter. Cell 37: 491–502

    Article  PubMed  CAS  Google Scholar 

  • Galas DJ, Schmitz A (1978) DNAse footprinting, a simple method for the detection of protein-DNA binding specificity. NAR 5: 3157–3170

    Article  PubMed  CAS  Google Scholar 

  • Goyne TE, Sigman DS (1987) Nuclease activity of 1,10-phenanthroline-copper ion. Chemistry of deoxyribose oxidation. J Am Chem Soc 109: 2846–2848

    Article  CAS  Google Scholar 

  • Graham DR, Sigman DS (1984) Zinc ion in E. coliDNA polymerase L A reinvestigation. Inorg Chem 23: 4188–4191

    Article  CAS  Google Scholar 

  • Groves JT, Subramanian DV (1984) Hydroxylation by cytochrome P-450 and metalloporphyrin models. Evidence for allylic rearrangement. J Am Chem Soc 106: 2177–2181

    Article  CAS  Google Scholar 

  • Jessee B, Gargiulo G, Razvi F, Worcel A (1982) Analgous cleavage of DNA by micrococcal nuclease and 1,10-phenanthroline-cuprous complex. NAR 10: 5823–5834

    Article  PubMed  CAS  Google Scholar 

  • Kirkegaard K, Buc H, Spassky A, Wang J (1983) Mapping of single-stranded regions in duplex DNA at the sequence level: single-strand specific cytosine methylation in RNA polymerase-promoter complexes. Proc Natl Acad Sci USA 80: 2544–2548

    Article  PubMed  CAS  Google Scholar 

  • Kuwabara MD, Sigman DS (1987) Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coliRNA polymerase-lac promoter complexes. Biochemistry 26: 7234–7238

    Article  PubMed  CAS  Google Scholar 

  • Kuwabara M, Yoon C, Goyne TE, Thederahn T, Sigman DS (1986) Nuclease activity of 1,10-phenanthroline-copper ion and its complexes with netropsin and EcoRI. Biochemistry 25: 7401–7408

    Article  PubMed  CAS  Google Scholar 

  • Marshall LE, Graham DR, Reich KA, Sigman DS (1981) Cleavage of DNA by the 1,10-phenanthroline-cuprous complex. Hydrogen peroxide requirement: primary and secondary structure specificity. Biochemistry 20: 244–250

    Article  PubMed  CAS  Google Scholar 

  • Pope LE, Sigman DS (1984) Secondary structure specifity of the nuclease activity of 1,10-phenanthroline-copper complex. Proc Natl Acad Sci 81: 3–7

    Article  PubMed  CAS  Google Scholar 

  • Pope LM, Reich KA, Graham DR, Sigman DS (1982) Products of DNA cleavage by the 1,10-phenanthroline copper complex. Identification of E. coliDNA polymerase I inhibitors. J Biol Chem 257: 12121–12128

    PubMed  CAS  Google Scholar 

  • Pribnow D (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72: 784–788

    Article  PubMed  CAS  Google Scholar 

  • Revzin A, Geglarek M, Garner M (1986) Comparison of nucleic acid-protein interactions in solution and in polyacrylamide gels. Anal Biochem 153: 172–177

    Article  PubMed  CAS  Google Scholar 

  • Scheffler LE, Elson EL, Baldwin RL (1968) Helix formation by dAT oligomers. I. Hairpin and straight-chain helices. J Mol Biol 36: 291–304

    Article  PubMed  CAS  Google Scholar 

  • Sigman DS, Graham DR, D’Aurora V, Stem AM (1979) Oxygen-dependent cleavage of DNA by the 1,10-phenanthroline-cuprous complex. Inhibition of E. coliDNA polymerase I. J Biol Chem 254: 12269–12272

    PubMed  CAS  Google Scholar 

  • Sigman DS, Spassky A, Rimsky S, Buc H (1985) Conformational analysis of lac promoters using the nuclease activity of 1,10-phenanthroline-copper ion. Biopolymers 24: 183–197

    Article  PubMed  CAS  Google Scholar 

  • Slater JP, Mildvan AS, Loeb LA (1971) Zinc in DNA polymerases. Biochem Biophys Res Commun 44: 37–43

    Article  PubMed  CAS  Google Scholar 

  • Spassky A (1986) Visualization of the movement of the Escherichia coliRNA polymerase along the lac UV5 promoter during the initiation of the transcription. J Mol Biol 188: 99–103

    Article  PubMed  CAS  Google Scholar 

  • Spassky A, Sigman DS (1985) Nuclease activity of 1,10-phenanthroline-copper ion. Conformational analysis and footprinting of the lac operon. Biochemistry 24: 8050–8056

    Article  PubMed  CAS  Google Scholar 

  • Spassky A, Sigman DS (1985) Nuclease activity of 1,10-phenanthroline-copper ion. Conformational analysis and footprinting of the lac operon. Biochemistry 24: 8050–8056

    Article  PubMed  CAS  Google Scholar 

  • Springgate CF, Mildvan AS, Abramson R, Engle JL, Loeb LA (1973) Escherichia coli deoxyribonucleic acid polymerase I, a zinc metalloenzyme. J Biol Chem 248:5987–5993

    PubMed  CAS  Google Scholar 

  • Thederahn T, Sigman DS (1988) Nuclease activity of 1,10-phenanthroline-copper. Kinetic mechanism. J Am Chem Soc (in press)

    Google Scholar 

  • Yoon C, Kuwabara M, Law R, Wall R, Sigman DS (1988) Sequence-dependent variability of DNA structure. J Biol Chem 263: 8458–8643

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Biology Institute and Department of Biological Chemistry, School of Medicine, University of California, Los Angeles, CA, 90024, USA

    D. S. Sigman

  2. Institut Pasteur, Paris, France

    A. Spassky

Authors
  1. D. S. Sigman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Spassky
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Abteilung Chemie, Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Straße 3, 3400, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, DD1 4HN, Dundee, UK

    David M. J. Lilley

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Sigman, D.S., Spassky, A. (1989). DNase Activity of 1,10-Phenanthroline-Copper Ion. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83709-8_2

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-83709-8_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83711-1

  • Online ISBN: 978-3-642-83709-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation