Abstract
Biological tissues are not homogenous; instead they consist of cells having specific functions. A typical bifacial leaf, for example, contains not only photosynthetic mesophyll cells (palisade parenchyma plus spongy parenchyma) but also epidermal, guard, and bundle-sheath cells, as well as myriad minor cell types. Their specific functions indicate that profound biochemical differences exist among adjacent cells. These differences are obliterated by tissue homogenation, which precedes most analytical biochemistry. Biologists have thus been challenged to develop methods that allow for selective sampling of specific cell types and analysis of the resultant small amounts of material.
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Hampp, R., Rieger, A., Outlaw, W.H. (1990). Microdissection and Biochemical Analysis of Plant Tissues. In: Linskens, HF., Jackson, J.F. (eds) Physical Methods in Plant Sciences. Modern Methods of Plant Analysis, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83611-4_5
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DOI: https://doi.org/10.1007/978-3-642-83611-4_5
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