High-Performance Liquid Chromatography of Catecholamines and their Metabolites in Biological Material
The use of high-performance liquid chromatography (HPLC) as an analytical tool for the life sciences has become increasingly important and the techniques and instrumentation involved are continuously developing to higher levels of sophistication. One result of this rapid progress is that a user of HPLC for a certain bioanalytical purpose is often faced with a great problem of selecting from the overwhelming menu offered in the field with respect to choice of column type, mobile phase, elution technique, detector system, degree of computerization etc. To this are then added the particular problems associated with the pre-chromatographic work, i.e. the extraction, prepurification and concentration steps often necessary to produce a sample suitable for injection into an HPLC-system.
KeywordsElectrochemical Detection Fluorimetric Detection Solvent Delivery System Alumina Extraction Postcolumn Derivatization
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