Molecular Genetics of Pathogenicity of Xanthomonas Campestris
Although genetical methods have been used to analyze microbial physiology with great success for several decades, complex phenomena such as pathogenicity present technical difficulties which have acted as a deterrent until recently, when the development of recombinant DNA procedures advanced to the point where novel approaches were feasible. An important milestone was the development of some broad host range gene cloning vectors [e.g. 1,2,3,4,5,6,7,] which are either known or presumed to be suitable for use with members of the several genera of Gram-negative bacterial plant pathogens (Erwinia, Pseudomonas and Xanthomonas). The choice of vector for a particular organism is usually empirical. Factors such as the stability of Plasmids in vivo are of some importance, and variations may be apparent even between different isolates of the same bacterial species.
KeywordsGlycine Pseudomonas Tetracycline Kanamycin Rhizobium
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