Molecular Genetics of Pathogenicity of Xanthomonas Campestris
Although genetical methods have been used to analyze microbial physiology with great success for several decades, complex phenomena such as pathogenicity present technical difficulties which have acted as a deterrent until recently, when the development of recombinant DNA procedures advanced to the point where novel approaches were feasible. An important milestone was the development of some broad host range gene cloning vectors [e.g. 1,2,3,4,5,6,7,] which are either known or presumed to be suitable for use with members of the several genera of Gram-negative bacterial plant pathogens (Erwinia, Pseudomonas and Xanthomonas). The choice of vector for a particular organism is usually empirical. Factors such as the stability of Plasmids in vivo are of some importance, and variations may be apparent even between different isolates of the same bacterial species.
KeywordsBroad Host Range Pathogenicity Gene Xanthomonas Campestris Avirulence Gene Cosmid Vector
Unable to display preview. Download preview PDF.
- 4.Bagdasarian, M., Lurz, R., Rückert, B., Franklin, F.C.H., Bagdasarian M.M., Frey, J. and Timmis, K.N., 1981, Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas, Gene, 16: 237–247.PubMedCrossRefGoogle Scholar
- 7.Tait, R.C., Close, T.J., Lundquist, R.C., Hagiya, M., Rodriguez, R.I. and Kado, C.I., 1983, Construction and characterization of a versatile broad host range DNA cloning system for Gram-negative bacteria, Bio Technology, 1: 269–275.Google Scholar
- 9.Daniels, M.J., Barber, C.E., Turner, P.C., Cleary, W.G. and Sawczyc, M.K., 1984, Isolation of mutants of Xanthomonas campestris pv. campestris showing altered pathogenicity, J. Gen. Microbiol., 130: 2447–2455.Google Scholar
- 12.Cook, A.A., Walker, J.C. and Larson, R.H., 1952, Studies on the disease cycle of black rot of crucifers, Phytopathology, 42: 162–167.Google Scholar
- 13.Bain, D.C., 1952, Reaction of Brassica seedlings to blackrot, Phytopathology, 42: 497–500.Google Scholar
- 14.Boucher, C., Barberis, P., Demery, D. and Trigalet, A., 1985, in: “Advances in Molecular Genetics of the Bacteria — Plant Interaction”, A.A. Szalay, and R.P. Legocki, eds., pp. 165–168, Cornell University, Ithaca, NY.Google Scholar
- 18.Gabriel, D.W., 1985, in: “Advances in Molecular Genetics of the Bacteria — Plant Interaction”, A.A. Szalay and R.P. Logocki, eds., pp. 202–203, Cornell University, Ithaca, NY.Google Scholar