Mechanism of Resistance of Wheat Against Stem Rust in the Sr5/P5 Interaction
Some of the wheat/stem rust interactions, e.g. the Sr5/P5 interaction, are characterized by a hypersensitive response of host cells, as first described by Stakman . These cells are easily detected with the aid of an epifluorescence microscope by their bright yellow colour. The fungus can be located following the fluorochromation technique developed by Rohringer and co-workers using the optical brighteners calcofluor or diaethanol [2,3]. In incompatible interactions conditioned by the Sr5 gene the majority of the colonies do not form more than two haustorial mother cells (HMC) [4,5]. Further growth is arrested by the rapid hypersensitive response of the penetrated host cells. Haustorial development is blocked at a very early stage. Hypersensitive host cells are killed rapidly. Fluorescence is not limited to the cell wall but extends over the whole protoplast. There is no hypersensitive response in incompatible interactions; haustoria become rather large in epidermal cells as well as in mesophyll cells .
KeywordsHydrolysis Lignin Electrophoresis Germinate Polyacrylamide
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