Advertisement

Mechanism of Resistance of Wheat Against Stem Rust in the Sr5/P5 Interaction

  • H. J. Reisener
  • R. Tiburzy
  • K. H. Kogel
  • B. Moerschbacher
  • B. Heck
Part of the NATO ASI Series book series (volume 1)

Abstract

Some of the wheat/stem rust interactions, e.g. the Sr5/P5 interaction, are characterized by a hypersensitive response of host cells, as first described by Stakman [1]. These cells are easily detected with the aid of an epifluorescence microscope by their bright yellow colour. The fungus can be located following the fluorochromation technique developed by Rohringer and co-workers using the optical brighteners calcofluor or diaethanol [2,3]. In incompatible interactions conditioned by the Sr5 gene the majority of the colonies do not form more than two haustorial mother cells (HMC) [4,5]. Further growth is arrested by the rapid hypersensitive response of the penetrated host cells. Haustorial development is blocked at a very early stage. Hypersensitive host cells are killed rapidly. Fluorescence is not limited to the cell wall but extends over the whole protoplast. There is no hypersensitive response in incompatible interactions; haustoria become rather large in epidermal cells as well as in mesophyll cells [3].

Keywords

Epidermal Cell Hypersensitive Response Mesophyll Cell Stem Rust Isogenic Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Stakman, E.C., 1915, Relation between Puccinia graminis and plants highly resistant to its attack, J. Agric. Res., 4: 193–200.Google Scholar
  2. 2.
    Rohringer, R., Kim, W.K., Samborski, D.J. and Howes, N., 1977, Calcofluor: An optical brightener of fluorescence microscopy of fungal parasites in leaves, Phytopathol., 67: 808–810.CrossRefGoogle Scholar
  3. 3.
    Kuck, K.H., Tiburzy, R., Haussier, C and Reisener, H.J., 1981, Visualization of rust haustoria in wheat leaves by using fluorochroms, Physiol. Plant Pathol., 19: 439–441.Google Scholar
  4. 4.
    Rohringer, R., Kim, W.K. and Samborski, D.J., 1979, A histological study of interaction between avirulent race of stem rust and wheat containing resistance genes Sr5, Sr6, Sr8, or Sr22, Can. J. Bot., 57: 324–331.CrossRefGoogle Scholar
  5. 5.
    Tiburzy, R., 1984, Untersuchungen zur Bedeutung der hypersensitiven Reaktion befallener Weizenzellen als Resistenz-faktor gegen Puccinia graminis f.sp. tritici, PhD Thesis, RWTH Aachen.Google Scholar
  6. 6.
    Tiburzy, R. and Reisener, H.J., 1984, Fluoreszenzmikroskopische Beobachtungen am Weizenschwarzrost, Zeiss Information, 27: 54–58.Google Scholar
  7. 7.
    Mayama, S., Matsuura, Y., Iida, H. and Tani, T., 1982, The role of avenalumin in the resistance of oat to crown rust, Puccinia coronata f.sp. avenae., Physiol. Plant Pathol., 20: 189–199.CrossRefGoogle Scholar
  8. 8.
    Callow, J.A., 1984, Cellular and molecular recognition between higher plants and fungal pathogens, in: “Encyclopedia of Plant Physiology, New Series, Vol. 17, Cellular Interactions”, H.F. Linskens and J. Heslop-Harrison, eds., pp. 212–237, Springer-Verlag, Berlin.Google Scholar
  9. 9.
    Kogel, K.H., Schrenk, F., Sharon, N. and Reisener, H.J., 1984, Suppression of the hypersensitive response in wheat stem rust interaction by reagents with affinity for wheat plasmamembrane galactoconjugates, J. Plant Physiol., 118: 343–352.Google Scholar
  10. 10.
    Kogel, K.H., Ehrlich-Rogozinski, S., Reisener, H.J. and Sharon, N., 1984, Surface galactolipids of wheat protoplats as receptors for soybean agglutinin and their possible relevance to host-parasite interaction, Plant Physiol., 76: 924–928.PubMedCrossRefGoogle Scholar
  11. 11.
    Kogel, K.H., Heck, B., Kogel, G., Moerschbacher, B. and Reisener, H.J., 1985, A fungal elicitor of the resistance response in wheat, Z. Naturf., 40c: 743–744.Google Scholar
  12. 12.
    Moerschbacher, B., Kogel, K.H., Noll, U. and Reisener, H.J., 1985, An elicitor of the hypersensitive response in wheat leaves isolated from the rust fungus Puccinia graminis f.sp. tritici. I. Isolation and partial characterization, Z. Naturforsch., 41c: in press.Google Scholar
  13. 13.
    Moerschbacher, B., Heck, B., Kogel, K.H., Obst, O. and Reisener, H.J., 1985, An elicitor of the hypersensitive lignification response in wheat leaves isolated from the rust fungus Puccinia graminis f.sp. tritici. II. Induction of enzymes correlated with the biosynthesis of lignin, Z. Naturforsch., in press.Google Scholar
  14. 14.
    Heck-Wattjes, B., 1986, Gelelektrophoretischer Vergleich Schwarzrost-resistenter und — suszeptibler isogener Weizenlinien (Triticum aestivum/ Puccinia graminis f.sp. tritici), PhD Thesis, RWTH Aachen.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • H. J. Reisener
    • 1
  • R. Tiburzy
    • 1
  • K. H. Kogel
    • 1
  • B. Moerschbacher
    • 1
  • B. Heck
    • 1
  1. 1.Institut für Biologie III (Pflanzenphysiologie)RWTH AachenAachenGermany

Personalised recommendations