Fast Atom Bombardment of Peptides Above 5000 Daltons

  • C. Fenselau
  • K. Hyver
Part of the Springer Proceedings in Physics book series (SPPHY, volume 9)


This paper summarizes analyses and investigations made through the last four years in a sector mass spectrometer with a 23 Kg magnet, transmitting ions up to about 3000 Daltons at full accelerating voltage. In order to work with ions above 5000 Daltons the accelerating voltage and often the resolution were lowered. Many of the considerations and much of the protocol we have developed to push this instrument to its limit will be applicable to higher mass magnets as these become available. The Kratos MS-50 used in this work was fitted with a saddle field gun for fast atom bombardment, an 8kV post accelerating detector, and the Kratos DS-55 data system with software written both in house and commercially. This instrument was routinely calibrated to assign masses in real time to about 4500 Daltons. Alternatively, the computer system could be calibrated through 3000 amu anywhere in the mass range up to at least 7500 Daltons for off-line mass assignment. Thus masses above 5000 were assigned either by peak matching or by computer supported off-line comparison to cesium iodide reference spectra.


Average Molecular Weight Fast Atom Bombardment Molecular Weight Determination Peak Match Perfl Uorohexane 
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  1. 1.
    J. Yergey, D. Heller, G. Hansen, R.J. Cotter and C. Fenselau: Anal. Chem. 55, 353 (1983).CrossRefGoogle Scholar
  2. 2.
    C. Fenselau, J. Yergey and D. Heller: Intern. J. Mass Spectrom. Ion Physics 53, 5 (1983).CrossRefGoogle Scholar
  3. 3.
    R.J. Cotter, B.S. Larsen, D.N. Heller, J.E. Campana and C. Fenselau: Anal. Chem. 57, 1479 (1985).CrossRefGoogle Scholar
  4. 4.
    K.L. Rinehart: Science 218, 254 (1982).CrossRefGoogle Scholar
  5. 5.
    R.D. MacFarlane: Biomedical Mass Spectrometry, ed. G.R. Waller and O.C. Dermer ( Wiley Interscience, New York, 1980 ).Google Scholar
  6. 6.
    B. Sundqvist, I. Kamensky, P. Hakansson, J. Kjellberg, M. Salehpour, S. Widdiyasekera, J. Fohlman, P.A. Peterson and P. Roepstorff: Biomed. Mass Spectrom. 11, 242 (1984).CrossRefGoogle Scholar
  7. 7.
    B.T. Chait and F.H. Field: Intern. J. Mass Spectrom. Ion Processes 65, 169 (1985).CrossRefGoogle Scholar
  8. 8.
    M. Alai, C. Fenselau, R.J. Cotter and C. Schwabe: Anal. Chem. in press.Google Scholar
  9. 9.
    D.L. Bunker and F.M. Wang: J. Amer. Chem. Soc. 99, 7457 (1977).CrossRefGoogle Scholar
  10. 10.
    P. Demirev, M. Alai, R. van Breemen, R. Cotter and C. Fenselau: Proceedings of the Fifth International Conference on Secondary Ion Mass Spectrometry, Springer-Verlag Series in Chemical Physics, 1986.Google Scholar
  11. 11.
    K.J. Hyver, J.E. Campana, R.J. Cotter and C. Fenselau: Biochem. Biophys. Res. Commun. 130 1287 (1985).Google Scholar
  12. 12.
    D.N. Heller, C. Fenselau, J. Yergey and R.J. Cotter: Anal. Chem. 56, 2274 (1984).CrossRefGoogle Scholar
  13. 13.
    C.R. Savage, T. Inagami and S. Cohen: J. Biol. Chem. 247, 7612 (1972).Google Scholar
  14. 14.
    C. Fenselau, D.N. Heller, M.S. Miller and H.B. White: Anal. Biochem. 150, (1985).Google Scholar
  15. 15.
    R.R. Townsend, D.N. Heller, C.C. Fenselau and Y.C. Lee: Biochemistry 23, 6389 (1984).CrossRefGoogle Scholar
  16. 16.
    Christian Schwabe, Department of Biochemistry, Medical University of South CarolinaGoogle Scholar
  17. 17.
    Philip Andrews, Department of Biochemistry, Purdue University.Google Scholar
  18. 18.
    R.J. Cotter: Anal. Chem. 56, 2594 (1984).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • C. Fenselau
    • 1
  • K. Hyver
    • 1
  1. 1.Department of PharmacologyJohns Hopkins University School of MedicineBatimoreUSA

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