Abstract
The frequent lack of correlation between endogenous levels of auxins and selected physiological processes which has often been reported in the literature suggests the conclusion that such correlations do not often exist. However, from a critical evaluation of the methods used for such studies, it is apparent that most of the qualitative and quantitative determinations made for auxins in general did not comply with good analytical practice. For example, some mass spectra (MS) of both reference and putative IAA derivatives that have been recently published in the literature contain gross errors (i.e., Arteca et al. 1980). In addition, many of the quantitative calculations have been based on nonspecific ions or detector signals.
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References
Allen JRF, Baker DA (1980) Free tryptophan and indole-3-acetic acid levels and vascular pathways of Ricinus communis L. Planta 148:69–74
Allen JRF, Holmstedt B (1980) The simple ß-carbolines. Phytochemistry 19:1573–1582
Allen JRF, Rebeaud JER, Rivier L, Pilet PE (1982a) Quantification of indole-3-acetic acid by GC-MS using deuterated labeled internal standard. In: Schmidt HL, Forstel HL, Heinziger K (eds) Stable isotopes. Elsevier, Amsterdam, pp 529–534
Allen JRF, Rivier L, Pilet PE (1982b) Quantification of indol-3-yl-acetic acid in pea and maize seedlings by gas chromatography-mass spectrometry. Phytochemistry 21:523–534
Arteca RN, Poovaiah BW, Smith OE (1980) Use of high performance liquid chromatography for the determination of endogenous hormone levels in Solanurn tuberosum L. subjected to carbon dioxide enrichment of the root zone. Plant Physiol 65:1216–1219
Atsumi S, Kuraishi S, Hayashi T (1976) An improvement of auxin extraction procedure and its application to cultured plant cells. Planta 129:245–247
Bandurski RS (1979) Chemistry and physiology of conjugates of indole-3-acetic acid. In: Mandava N (ed) Plant growth substances. Am Chem Soc Symp Ser 111:1–17
Bandurski RS (1984) Metabolism of indole-3-acetic acid. In: Crozier A, Hillman JR (eds) The biosynthesis and metabolism of plant hormones. Cambridge, Soc of Exp Biol, Sem Ser 23:183–200
Bearder J (1980) Plant hormones and other growth substances — their background, structure and occurrence. In: MacMillan J (ed) Hormonal regulation of development I. Encyclopedia of plant physiology new serie, vol 9. Springer, Berlin Heidelberg New York, pp 9–112
Bialek K, Meudt WJ, Cohen JD (1983) Indole-3-acetic acid (IAA) and IAA conjugates applied to been stem sections. Plant Physiol 73:130–134
Budzikiewicz H, Djerassi C, Williams DH (1964) Interpretation of mass spectra of organic compounds. Holdan-Day, San Fransisco, pp 251–253
Bush ED, Trager WF (1981) Analysis of linear approaches to quantitative stable isotope methodology in mass spectrometry. Biomed Mass Spectrometry 8:211–218
Caruzo JL, Zeisler CS (1983) Indole-3-acetic acid in douglas fir seedlings: a reappraisal. Phytochemistry 22:589–590
Chapman JR (1978) Computers in mass spectrometry. Academic Press, London New York San Fransisco, pp 265
Cohen JD (1984) Convenient apparatus for the generation of small amounts of diazomethane. J Chromatogr 303:193–196
Cohen JD, Bandurski RS (1982) Chemistry and physiology of the bound auxins. Ann Rev Plant Physiol 33:403–430
Cohen JD, Bialek K (1984) The biosynthesis of indole-3-acetic acid in higher plants. In: Crozier A, Hillman JR (eds) The biosynthesis and metabolism of plant hormones. Cambridge, Soc Exp Biol, Sem Ser 23:165–181
Cohen JD, Schulze A (1981) Double-standard isotope dilution assay. I. Quantitative assay of indole-3-acetic acid. Anal Biochem 112:249–257
Dawson PH (ed) (1976) Quadrupole mass spectrometry and its applications. Elsevier, Amsterdam Oxford New York, pp 349
Elliott MC, Stowe BB (1971) Indole compounds related to auxins and goitrogens of wood (Isatis tinctoria L.). Plant Physiol 47:366–372
Engvild KC, Egsgaard H, Larsen E (1981) Determination of 4-chloroindoleacetic acid methyl ester in Viciae species by gas chromatography-mass spectrometry. Physiol Plant 53:79–81
Epstein E, Cohen JD (1981) Microscale preparation of pentafluorobenzyl ester: electron-capture GC detection of IAA from plants. J Chromatogr 209:413–420
Epstein E, Cohen JD, Bandurski RS (1980) Concentration and metabolic turnover of in-doles in germinating kernels of Zea mays L. Plant Physiol 65:415–421
Freeman RR (ed) (1979) High resolution gas chromatography. Hewlett-Packard, Avondale, pp 162
Harrison AG (1983) Chemical ionization mass spectrometry. Chemical Rubber Company Press, Boca Raton, pp 630
Hofinger M (1980) A method of quantification of indole auxins in the picogram range by high performance GC of their heptafluorobutyryl derivative. Phytochemistry 19:219–221
Iino M (1982) Action of red light on IAA status and growth in coleoptiles of etiolated maize seedlings. Planta 156:21–32
Iino M, Yu ST, Carr DJ (1980) Improved procedure for the estimation of nanogram quantities of indole-3-acetic acid in plant extracts using the indolo-pyrone fluorescence method. Plant Physiol 66:1099–1105
Jamieson WD, Hutzinger O (1970) Identification of simple naturally occuring indoles by mass spectrometry. Phytochemistry 9:2029–2036
Jennings W (1980) Gas chromatography with capillary columns 2nd ed. Academic Press, New York, pp 184
Knox JP, Wareing PF (1984) Apical dominance in Phaseolus vulgaris L.: the possible roles of abscisic acid and indole-3-acetic acid. J Exp Bot 35:239–244
Letham DS, Goodwin PB, Higgins TJV (ed) Phytohormones and related compounds, a treatise, vol 1: the biochemistry of phytohormones and related compounds, and vol 2: phytohormones and the development of higher plants. Elsevier, Amsterdam
MacMillan J (ed) (1980) Hormonal regulation of development I. Encyclopedia of plant physiology new serie, vol 9. Springer, Berlin Heidelberg New York, pp 681
Magnus V, Bandurski RS, Schultze A (1980) Synthesis of 4,5,6,7 deuterium-labeled IAA for use in mass spectrometric assays. Plant Physiol 66:775–781
Mann JD, Jaworski EG (1970) Minimizing loss of indoleacetic acid during purification of plant extracts. Planta 92:285–291
Martin GC, Scott IM, Neill SJ, Horgan R (1982) Identification of abscisic acid glucose ester, indole-3-acetic acid, zeatin and zeatin riboside in receptacles of pear. Phytochemistry 21:1079–1082
McDougall J, Hillman JR (1978) Analysis of indole-3-acetic acid using GC-MS techniques. In: Hillman JR (ed) Isolation of plant growth substances. Cambridge University Press, London, pp 1–25
McDougall J, Hillman JR (1980) Derivatives of indole-3-acetic acid for STM GC-MS studies. Z Pflanzenphysiology 98:89–93
McFadden WH (ed) (1973) Techniques of combined gas chromatography-mass spectrometry: application in organic analysis. Wiley, New York London Sydney Toronto, pp 463
Millard BJ (1978) Quantitative mass spectrometry. Heyden, London Philadelphia Rheine, pp 171
Munson B (1977) Chemical ionization mass spectrometry: ten years after. Analyt Chem 49:772A–778A
Noma M, Koike N, Sano M, Kawashima N (1984) Endogenous indole-3-acetic acid in the stem of tobacco in relation to flower neoformation as measured by mass spectrometric assay. Plant Physiol 75:257–260
Nonhebel HM, Bandurski RS (1984) Oxidation of indole-3-acetic acid and oxindole-3acetic acid to 2,3-dihydro-7-hydro-2-oxo-1 H indole-3-acetic acid-7’-O-ß-glucopyranoside in Zea mays seedlings. Plant Physiol 76:979–983
Nonhebel HM, Crozier A, Hillman JR (1983) Analysis of [14C]indole-3-acetic acid metabolites from the primary roots of Zea mays seedlings using reverse-phase high-performance liquid chromatography. Physiol Plant 57:129–134
Pengelly WL, Hall PJ, Schulze A, Bandurski RS (1982) Distribution of free and bound indole-3-acetic acid in the cortex and stele of Zea mays mesocotyl. Plant Physiol 69:1304–1307
Pilet PE, Rebeaud JER (1983) Effect of abscisic acid on growth and indolyl-3-acetic acid levels in maize roots. Plant Sci Lett 31:117–122
Pilet PE, Saugy M (1985) Effect of applied and endogenous IAA on maize root growth. Planta 164:254–258
Plett T, Böttger M, Hedden P, Graebe J (1984) Occurrence of 4-C1-indoleacetic acid in broad bean and correlation of its levels with seed development. Plant Physiol 74:320–323
Powers JC (1968) The mass spectrometry of simple indoles. J Org Chem 5:2044–2050
Purcell JE (1982) Quantitative capillary gas chromatographic analysis. Chromatographia 15:546–558
Rayles DL, Purves WK (1967) Isolation and identification of indole-3-ethanol (tryptophol) from cucumber seedlings. Plant Physiol 42:520–524
Reeve DR, Crozier A (1980) A quantitative analysis of plant hormones. In: MacMillan J (ed) Hormonal regulation of development I. Encyclopedia of plant physiology new serie vol 9. Springer, Berlin Heidelberg New York, pp 203–280
Reinecke DM, Bandurski RS (1983) Oxindole-3-acetic acid, an IAA catabolite in Zea mays L. Plant Physiol 71:211–213
Rivier L, Pilet PE (1971) Composés hallucinogènes indoliques naturels. Ann Biol 10:129–149
Rivier L, Pilet PE (1974) Indolyl-3-acetic acid in cap and apex of maize roots: identification and quantification by mass fragmentography. Planta 120:107–112
Rivier L, Pilet PE (1983) Simultaneous gas chromatographic-mass spectrometric determination of abscisic acid and indol-3y1-acetic acid in the same plant tissue using 2H-labelled internal standard. In: Frigerio A (ed) Recent developments in mass spectrometry in biochemistry, medicine and environmental research, vol 8. Elsevier, Amsterdam, pp 219–231
Rivier L, Saugy M (1986) Chemical ionization mass spectrometry of IAA and ABA: evaluation of negative ion detection and quantification of ABA in growing maize roots. J Plant Growth Regul, in press
Salvidge RA, Wareing PL (1983) Seasonal variation in endogenous indole-3-acetic acid and abscisic acid levels in Pinus conforta Dougl. Can J Forest Res 23:123–134
Sandberg G (1984) Biosynthesis and metabolism of indole-3-ethanol and indole-3-acetic acid by Pinus sylvestris L. needles. Planta 161:1–6
Sandberg G, Jensen E, Crozier A (1984) Analysis of 3-indole carboxylic acid in Pinus sylvestris needles. Phytochemistry 23:99–102
Sandberg G, Crozier A, Ernstsen A (1986) Indole-3-acetic acid and related compounds. In: Rivier L, Crozier A (eds) Principles and practice of plant hormone analysis. Academic Press, London Oxford, in press
Saugy M, Pilet PE (1984) Enodgenous indol-3yl-acetic acid in stele and cortex of gravi-stimulated maize roots. Plant Sci Lett 37:93–99
Schneider EA, Wightman F (1978) Auxins. Phytohormones and related compounds — a comprehensive treatise. In: Letham DS, Goodwin PB, Higgins TJV (eds) The biochemistry of phytohormones and related compounds, vol 1. Elsevier/North-Holland, Amsterdam, pp 29–105
Schneider EA, Kazakoff CW, Wightman F (1985) Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedlings organs. Planta 165:232–241
Scott TK (ed) (1984) Hormonal regulation of development II: the functions of hormones from the level of the cell to the whole plant. Encyclopedia of plant physiology new serie, vol 10. Springer, Berlin Heidelberg New York Tokyo, pp 309
Seeley SD, Powell LE (1973) Gas chromatography and detection of microquantities of gibberellins and IAA as their fluorinated derivatives. Anal Biochem 58:39–46
Segal LM, Wightman F (1982) Gas chromatographic and GC-MS evidence for the occurrence of 3-indolylpropionic acid and 3-indolylacetic acid in seedlings of Cucurbita pepo. Physiol Plant 56:367–370
Smith TA (1977) Tryptamine and related compounds in plants. Phytochemistry 16:171–175
Sundberg B, Sandberg G, Jensen E (1985) Identification and quantification of 3-indole methanol in etiolated seedlings of Scots pine (Pinus sylvestris). Plant Physiol 77:952–955
Wightman F, Lichty DL (1982) Identification of phenylacetic acid as a natural auxin in the shoots of higher plants. Pyhsiol Plant 55:17–24
Yokota T, Murofushi N, Takahashi N (1980) Extraction, purification and identification. In: MacMillan J (ed) Hormonal regulation of development I. Encyclopedia of plant physiology, new serie, vol 9. Springer, Berlin Heidelberg New York, pp 113–201
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Rivier, L. (1986). GC-MS of Auxins. In: Linskens, H.F., Jackson, J.F. (eds) Gas Chromatography/Mass Spectrometry. Modern Methods of Plant Analysis, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82612-2_8
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DOI: https://doi.org/10.1007/978-3-642-82612-2_8
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