Analysis of Malignant Effusions by Cellular Composition, Proliferation Kinetics, and In Vitro Clonogenicity
The human tumor clonogenic assay (HTCA) has received wide international attention and has been introduced in numerous laboratories since its original description by Hamburger and Salmon (1977). The dominant adavantage of the HTCA is its specificity for selecting neoplastic clones to the exclusion of other cellular elements. The neoplastic origin of cells comprising colonies has been confirmed by light and electron microscopy (Salmon and Liu 1979; Harris et al. 1982), production of tumor markers (Von Hoff et al. 1980), cytogenetics (Trent and Salmon 1980), and colony transplants into nude mice. Furthermore, it is believed that tumor clones growing in semisolid agar represent the cell fraction responsible for in vivo tumor propagation.
KeywordsAgar Adenocarcinoma Heparin Prostaglandin Myeloma
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