Analysis of Malignant Effusions by Cellular Composition, Proliferation Kinetics, and In Vitro Clonogenicity
The human tumor clonogenic assay (HTCA) has received wide international attention and has been introduced in numerous laboratories since its original description by Hamburger and Salmon (1977). The dominant adavantage of the HTCA is its specificity for selecting neoplastic clones to the exclusion of other cellular elements. The neoplastic origin of cells comprising colonies has been confirmed by light and electron microscopy (Salmon and Liu 1979; Harris et al. 1982), production of tumor markers (Von Hoff et al. 1980), cytogenetics (Trent and Salmon 1980), and colony transplants into nude mice. Furthermore, it is believed that tumor clones growing in semisolid agar represent the cell fraction responsible for in vivo tumor propagation.
KeywordsLabel Index Malignant Effusion Proliferation Kinetic Semisolid Agar Neoplastic Clone
Unable to display preview. Download preview PDF.
- Durie BGM, Salmon SE (1980) Cell kinetic analysis of human tumor stem cells. In: Salmon SE (ed) Cloning of human tumor stem cells. Liss, New York, pp 153–163Google Scholar
- Hofmann V, Berens ME, Martz G (to be published) Drug selection for perioperative chemotherapy. In: Metzger U, Senn HJ, Largiader F (eds) Recent results in cancer research.Google Scholar
- Kurland JI, Broxmeyer HE, Bockman RS, Moore MAS (1979) Role of monocyte-macrop age-derived colony stimulating factor and prostaglandin E in the positive and negative feedback control of myeloid stem cell proliferation. Blood 52: 388–407Google Scholar
- Meyskens FL (1980) Human melanoma colony formation in soft agar. In: Salmon SE (ed) Cloning of human tumor stem cells. Liss, New York, pp 85–99Google Scholar
- Von Hoff DD, Harris GJ, Johnson G, Glaubiger D (1980) Initial experience with the human tumor stem cell assay system: potential and problems. In: Salmon SE (ed) Cloning of human tumor stem cells. Liss, New York, pp 113–124Google Scholar