An Automated Multiple-Chamber Intermittent-Flow Respirometer

Abstract

Numerous types of respirometers, using a polarographic oxygen sensor (POS), have been described in the literature [1–4,7–11] and this book also presents a selection of different designs (Chaps. II.1–II.9, III.3–III.5). Although the method is widely used, no commonly accepted design has so far evolved. A few types of respirometers are available commercially, but these are either custom designs, made to order for a research establishment, or simple closed-system respirometers for BOD or work with cell suspensions. There is no general purpose aquatic respirometer available that is comparable in distribution and scope to the WARBURG or the GILSON in the field of gasometric respirometry. One reason may well be that it is a relatively easy matter to put together a workable system involving a POS, especially for short-term experiments where a simple closed system suffices (Chap. II.6). On the other hand, there are cases where respirometer design has to be matched to a specific and difficult measuring task and the requirements of a particular organism. Mostly such studies are undertaken by researchers well versed in the use of POS, who are able and willing to design and build their own equipment. Nevertheless, in a wide range of applications, routine measurement of oxygen consumption is only part of a more comprehensive investigation, where the experimenter may have no experience in the use of POS and the design of a suitable respirometer. What is required in this case is a respirometer as a standard laboratory instrument.