Effects of Dietary Cholesterol and Saturated Fat on the Appearance of HDLc in Peripheral and Cardic Lymph

  • P. Julien
  • A. Angel
Conference paper


Several studies on the kinetics of plasma LDL clearance and turnover indicate that up to 30 to 40% of LDL and HDL distribute in extravascular pools (1,2). Apoproteins known to be associated with VLDL, LDL and HDL have been detected in human peripheral (pedal) lymph by Reichl et al (3). In previous work, we have demonstrated that cholesterolrich lipoproteins, LDL and HDL, are present in cardiac lymph of pigs and dogs at concentrations 36 to 59% that of plasma and that cardiac lymph contained VLDL-like particles (4). Dietary effects on lipoproteins in peripheral lymph were observed by Courtice et al (5) who cannulated lymphatic vessels in hind limbs of hyperlipoproteinemic rabbits. Presumably lipoproteins in the interstitial space can undergo metabolic transformations because of the presence of lipid metabolizing enzymes (6) and because of proximity to lipoprotein binding sites on various parenchymal and mesenchymal cells.


Plasma Lipoprotein Peripheral Lymph Cholesterol Feeding Lymph Lipid Cholesterol Carrier 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Sniderman AD, Carew TE, Steinberg D (1975) Turnover and tissue distribution of 125I-labelled low density lipoprotein in swine and dogs. J Lipid Res 16: 293–299PubMedGoogle Scholar
  2. 2.
    Shepherd J, Packard CJ, Patsch JR, Gotto AM and Taunton OD (1978) 61:1582–1592Google Scholar
  3. 3.
    Reichl D, Postiglione A, Myant NB, Pflug JJ Press M (1975) Observation on the passage of apoproteins from plasma lipoproteins into peripheral lymph in two men. Clin Sci Mol Med 49: 419–426PubMedGoogle Scholar
  4. 4.
    Julien P, Angel A (1981) Composition and metabolism of very low density lipoproteins in dog cardiac lymph. Can J Biochem 59: 709–714PubMedCrossRefGoogle Scholar
  5. 5.
    Courtice FC, Munoz-Marcus M, Garlick DG (1964) The permeability of the blood capillaries of the leg to the lipoproteins in various hyperlipaemic states in the rabbit. Q J Exp Physiol 49: 441–456Google Scholar
  6. 6.
    Julien P, Angel A (1981) Lipoprotein metabolizing enzymes in cardiac lymph. Arteriosclerosis 1: 381A–382AGoogle Scholar
  7. 7.
    Mahley RW, Weisgraber KH, Innerarity T (1974) Canine lipoproteins and atherosclerosis. II Characterization of the plasma lipoproteins associated with atherogenic hyperlipodemia. Circ Res 35: 722–733PubMedGoogle Scholar
  8. 8.
    Sykes M, Cnoop-Koopmans WM, Julien P, Angel A (1981) The effects of hypothyroidism, age and nutrition on LDL catabolism in the rat. Metabolism 30: 733–738PubMedCrossRefGoogle Scholar
  9. 9.
    DeLamatre JG, Roheim PS (1981) Effect of cholesterol feeding on apo B and apo E concentrations and distributions in euthyroid and hypothyroid rats. J Lipid Res 22: 290–306Google Scholar
  10. 10.
    Parving HH, Hansen JM, Steen LN (1979) Mechanism of edema formation in myxedema - Increased protein extravasation and relatively slow lymphatic drainage. N Engl J Med 301:460–465PubMedCrossRefGoogle Scholar
  11. 11.
    Julien P, Downar E, Angel A (1981) Lipoprotein composition and transport in the pig and dog cardiac lymphatic system. Circ Res 49: 248–254PubMedGoogle Scholar
  12. 12.
    Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation of purification of total lipids from animal tissues. J Biol Chem 226: 497–509PubMedGoogle Scholar
  13. 13.
    Bartlett GR (1959) Phosphorus assay in colum chromatography. J Biol Chem 234: 466–468PubMedGoogle Scholar
  14. 14.
    Angel A, D’Costa MA, Yuen R (1979) Low density lipoprotein binding, internalization, and degradation in human adipose cells. Can J Biochem 57: 578–587PubMedCrossRefGoogle Scholar
  15. 15.
    Schonfeld G, Patsch W, Rudel LL, Nelson C, Epstein M, Olson RE (1982) Effects of dietary cholesterol and fatty acids on plasma lipoproteins. J Clin Invest 69: 1072–1080PubMedCrossRefGoogle Scholar
  16. 16.
    Mahley RW, Hui DY, Innerarity TL, Weisgraber KH (1981) Two independent lipoprotein receptors on hepatic membranes of dog, swine and man. Apo-B,E and apo-E receptors. J Clin Invest 68: 1197–1206PubMedCrossRefGoogle Scholar
  17. 17.
    Fong B, Rodrigues P, Julien P, Angel A (1982) Lipoprotein receptors in adult human adipocyte plasma membranes. Clin Res 30: 523AGoogle Scholar
  18. 18.
    Angel A, Farkas J (1974) Regulation of cholesterol storage in adipose tissue. J Lipid Res 15: 491–499PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • P. Julien
  • A. Angel

There are no affiliations available

Personalised recommendations