Abstract
The morphology of vitreous membranes from enucleated human eyes containing vitreous haemorrhage was studied by electron microscopy. Three types of membrane are described, based on their cellular composition: haematogenous, fibroblastic and neovascular. Simple vitreous haemorrhages usually failed to stimulate a fibroblastic cellular response, whereas vitreous blood clots in eyes with penetrating injuries were frequently invaded by choroidal and/or scleral fibroblasts. Fibroblast-like cells were also found in neovascular membranes, but not as a major cellular component. They had the appearances of astrocytes, suggesting an origin from the retina or optic disc in association with the intravitreal new vessel growth.
These data suggest that two factors are necessary for intravitreal fibrosis: an adequate port of entry for cellular invasion and a suitable substratum on which migrating cells can crawl.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Balazs EA, Darzynkiewicz Z (1973) The effect of hyaluronic acid on fibroblasts, mononuclear phagocytes and lymphocytes. In “The Biology of the Fibroblast”. Eds, Kulonen E and Pikkarain-en J Pp 237–254, Academic Press, New York and London
Cleary PE, Ryan SJ (1979) Experimental posterior penetrating eye injury to the rabbit. II Histology of wound, vitreous and retina. Brit J Ophthal 63: 312–321
Cleary PE, Ryan SJ (1979) Histology of wound, vitreous and retina in experimental posterior penetrating eye injury in the rhesus monkey. Amer J Ophthal 88: 221–231
Cliff WJ (1963) Observations on healing tissue: a combined light and electron microscopic investigation. Phil Trans B 246: 305–325
Constable IJ (1975) The pathology of vitreous membranes. Trans Ophthal Soc UK 95: 382–386
Duke-Elder S, Dobree JH (1977) Diseases of the retina. In: “System of Ophthalmology” Volume 10, p 150. Henry Kimpton London
Faulborn J, Topping TM (1978) Proliferation irr the vitreous cavity after perforating injuries. A histopathological study. A von Graefes Arch klin exp Opthal 205: 157–177
Foos RJ (1977) Vitreoretinal juncture over retinal vessels. A von Graefes Arch Klin exp Ophthal 204:233–234
Forrester JV, Lee WR, Williamson J (1978) The pathology of vitreous haemorrhage. I Gross and histological appearances. Arch Ophthal 96: 703–710
Forrester JV, Grierson I, Lee WR (1979) The pathology of vitreous haemorrhage II. Ultrastructure Arch Ophthal 97: 2368–2374
Forrester JV, Grierson I, Lee WR (1980) Vitreous membrane formation after experimental vitreous haemorrhage. A von Graefes Arch klin exp Ophthal 212: 227–242
Forrester JV, Lackie JM Inhibition of neutrophil adhesion by hyaluronic acid (in press) J. Cell Sci
Forrester JV, Wilkinson PC (in press) Inhibition of neutrophil locomotion by hyaluronic acid. J Cell Sci
Frielich, DB, Lee PF, Freeman HM (1966) Experimental retinal detachment. Arch Ophthal 20:432–436
Hogan MJ, Zimmerman LE (1960) Opthalmic Pathology p 650 WB Saunders Co, Philadelphia
Hogan MJ, Alvarado JA, Weddell JE (1971) Histology of the Human Eye p 613 WB Saunders Co Philadelphia
Klien BA (1938) Retinitis proliferans. Clinical and histological studies. Arch Ophthal 20: 427–436
Michaelson IC, Kraus J (1943) War injuries of the eye. Brit J Ophthal 27: 449–461
Oguchi L (1913) Über die Wirkung von Blutinjektionen in den Glaskörper nebst Bemerkungen über die sog. Retinitis proliferans. A von Graefes Arch klin exp Ophthal. 84: 446–520
Rentsch FJ (1977) The ultrastructure of preretinal macular fibrosis. A von Graefes Arch klin exp Ophthal 203: 321–337
Smith RS, van Heuven WAJ, Streeten B (1976) Vitreous membranes: a light and electron microscopical study. Arch Ophthal 94: 1556–1560
Treacher-Collins E (1929) Formative fibrous tissue reaction. Trans Ophthal Soc UK 49: 166–203
Underhill C, Dorfman A (1978) The role of hyaluronic acid in intercellular adhesion of cultured mouse cells. Exp Cell Res 117: 155–164
Yashamita T, Cibis P (1961) Experimental retinitis proliferans in the rabbit. Arch Ophthal 65: 49–58
Zinn KM, Constable IJ, Schepens CL (1977) The fine structure of human vitreous membranes. In: “Vitreous surgery and advances in fundus diagnosis and treatment”. Eds Freeman HM, Hirose T, and Schepens CL pp 39–49, New York
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Springer-Verlag Berlin · Heidelberg
About this paper
Cite this paper
Forrester, J.V., Lee, W.R. (1981). Cellular Composition of Post-haemorrhagic Opacities in the Human Vitreous. In: Lee, W.R. (eds) Transactions of the 8th Annual Meeting of the European Club for Ophthalmic Fine Structure in West Berlin, Maren 28 and 29,1980. Current Research in Ophthalmic Electron Microscopy, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81614-7_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-81614-7_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-10651-7
Online ISBN: 978-3-642-81614-7
eBook Packages: Springer Book Archive