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Structure and Nucleotide Sequence of the Cytochrome B Gene in Yeast Mitochondrial DNA

  • A. Tzagoloff
  • F. G. Nobrega
Part of the Colloquium der Gesellschaft für Biologische Chemie book series (MOSBACH, volume 31)

Abstract

The mitochondrial respiratory chain contains two distinct cytochromes of the b type (bk and bt). Cytochromes bk and bt differ in their redox potentials and absorption properties in the visible spectrum (Table 1). Both cytochromes are part of the coenzyme QH2-cytochrome c reductase and have been implicated to function on the main pathway of the electron transport chain (Rieske, 1976). Despite the fact that cytochrome b has been isolated from fungal (Weiss and Ziganke, 1974) and mammalian (VonJagow et al., 1978) sources, the relationship of the purified protein to cytochromes bk and bt has not been satisfactorily resolved and until recently it was not clear whether they represent two different or a single heme protein.

Keywords

Glutamic Acid tRNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alexander NJ, Vincent RD, Perlman PS, Miller DH, Hanson DK, Mahler HR (1979) Genetic and biochemical characterization of some mutant types affecting apocytochrome b and cytochrome oxidase. J Biol Chem 254: 2471–2479PubMedGoogle Scholar
  2. Chance B, Wilson DF, Dutton PL, Erecinska M (1970) Energy-coupling mechanisms in mitochondria: Kinetic spectroscopic and thermodynamic properties of an energytransducing form of cytochrome b. Proc Natl Acad Sci USA 66: 1175–1182Google Scholar
  3. Church GM, Gilbert W (1980) Yeast mitochondrial intron products required in trans for RNA splicing. In: Joseph DR, Schultz J, Scott WA, Werner R (eds) Mobilization and reassembly of genetic information. Academic Press, London New York, in pressGoogle Scholar
  4. Claisse ML, Spyridakis A, Wambier-Kluppel ML, Pajot P, Slonimski PP (1978) Analysis of proteins translated from the box region. In: Bacila M, Horecker BL, Stoppani AOM (eds) Biochemistry and genetics of yeast: Pure and applied aspects. Academic Press, London New York, pp. 369–390Google Scholar
  5. Dujon B (1980) Nucleotide sequences of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains with different alleles at the omega and ribl genetic loci. Cell submitted for publicationGoogle Scholar
  6. Grivell LA, Arnberg AC, Boer PH, Borst P, Bos JL, van Bruggen EFJ, Groot GSP, Hecht NB, Hensgens LAM, van Ommen GJB, Tabak HF (1979) Transcripts of yeast mitochondrial DNA and their processing. In: Cummings DJ, Borst P, Dawid IB, Weissman SM, Fox CF (eds) Extrachromosomal DNA: ICN-UCLA symposia on molecular and cellular biology, vol XV. Academic Press, London New York, pp 305–324Google Scholar
  7. Haid A, Schweyen RJ, Bechmann H, Kaudewitz F, Solioz M, Schatz G (1979) The mitochondrial COB region in yeast codes for apocytochrome b and is mosaic. Eur J Biochem 94: 451–464PubMedCrossRefGoogle Scholar
  8. Jacq C, Lazowska J, Slonimski PP (1980) Sur un nouveau mecanism de la regulation de l’expression genetique. CR Acad Sci Paris Ser D t290: 1–4Google Scholar
  9. Katan MB, van Harten-Loosbroek N, Groot GSP (1976a) The cytochrome bcl complex of yeast mitochondria. Eur J Biochem 65: 95–105PubMedCrossRefGoogle Scholar
  10. Katan MB, van Harten-Loosbroek N, Groot GSP (1976b) The cytochrome bcl complex of yeast mitochondria: Site of translation of the polypeptides in vivo. Eur J Biochem 70: 409–417CrossRefGoogle Scholar
  11. Lin LF, Clejan L, Beattie DS (1978) The synthesis of cytochrome b on mitochondrial ribosomes in Baker’s yeast. Eur J,Biochem 87: 171–179CrossRefGoogle Scholar
  12. Maxam A, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74: 560–564PubMedCrossRefGoogle Scholar
  13. Morimoto R, Rabinowitz M (1979) Derivation of the fine structure map of strain D273–10B and comparison with a strain (MH41–7B) differing in genome size. Mol Gen Genet 170: 25–48PubMedGoogle Scholar
  14. Nobrega FG, Tzagoloff A (1980a) Complete restriction map of the cytochrome b region on mitochondrial DNA in Saccharomyces cereVisiae D273–10B. J Biol Chem submitted for publicationGoogle Scholar
  15. Nobrega FG, Tzagoloff A (1980b) DNA sequence and organization of the cytochrome b gene in Saccharomyces cereVisiae D273–10B. J Biol Chem submitted for publicationGoogle Scholar
  16. Rieske JS (1976) Composition and function of complex III of the respiratory chain. Biochim Biophys Acta 456: 195–247PubMedGoogle Scholar
  17. Slonimski PP, Tzagoloff A (1976) Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and/or coenzyme QH2-cytochrome c reductase. Eur J Biochem 61: 27–41PubMedCrossRefGoogle Scholar
  18. Slonimski PP, Pajot P, Jacq C, Foucher M, Perrodin G, Kochko A, Lamouroux A (1978) Genetic, physical and complementation maps of the box region. In: Bacila M, Horecker BL, Stoppani AOM (eds) Biochemistry and genetics of yeast: Pure and applied aspects. Academic Press, London New York, pp 339–368Google Scholar
  19. Tzagoloff A, Akai A, Needleman RB, Zulch G (1975) Cytoplasmic mutants of Saccharomyces cerevisiae with lesions in enzymes of the respiratory chain and in the mitochondrial ATPase. J Biol Chem 250: 8236–8242PubMedGoogle Scholar
  20. Tzagoloff A, Foury F, Akai A (1976) Genetic loci on mitochondrial DNA involved in cytochrome b biosynthesis. Mol Gen Genet 149: 33–42PubMedCrossRefGoogle Scholar
  21. VonJagow G, Schagger H, Engel WD, Machleidt W, Machleidt I, Koff HJ (1978) Beef heart complex III: Isolation and characterization of cytochrome b. FEBS Lett 91: 121–125CrossRefGoogle Scholar
  22. Weiss H, Ziganke B (1974) Cytochrome b in Neurospora crassa mitochondria: Site of translation of the heure protein. Eur J Biochem 41: 63–71PubMedCrossRefGoogle Scholar
  23. Weiss H, Sebald W, Schwab AJ, Kleinow W, Lorenz B (1973) Contribution of mitochondrial and cytoplasmic protein synthesis to the formation of cytochrome b and cytochrome aa3. Biochimie 55: 815–821PubMedCrossRefGoogle Scholar
  24. Wesolowski M, Fukuhara H (1979) The genetic map of transfer RNA genes in yeast mitochondria: Correction and extension. Mol Gen Genet 170: 261–275PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • A. Tzagoloff
  • F. G. Nobrega
    • 1
  1. 1.Department of Biological SciencesColumbia UniversityNew YorkUSA

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