Abstract
The cell membrane has been the focus of much recent biochemical and biophysical research, primarily because of its role in cellular phenomena. Numerous efforts have been directed toward determining the motional state of the lipid components of the membrane, the motivation being the contention that the lipid bilayer is the basic matrix in which membrane proteins are embedded to form the biological membrane. As such, it is likely that such diverse phenomena as maintenance of ionic gradients and transmembrane potentials, activities of membrane-bound enzymes, interactions between membrane proteins, transmembrane signal transmission, intercellular communication, and manifestations of cellular development and cell transformation all depend on the structure and fluidity of the lipid bilayer.
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References
Abragam, A.: The Principles of Nuclear Magnetism. London: Oxford University Press 1961.
Anderson, P.W.: A mathematical model for the narrowing of spectral lines by exchange or motion. J. Phys. Soc. Jpn. 9, 316–339 (1954).
Berden, J.A., Barker, R.W., Radda, G.K.: NMR studies on phospholipid bilayers. Some factors affecting lipid distribution. Biochim. Biophys. Acta 375, 186–298 (1975).
Bloom, M., Burneil, E.E., Valic, M.I., Weeks, G.: Nuclear magnetic resonance line shapes in lipid bi-layer model membranes. Chem. Phys. Lipids 14, 107–112 (1975).
Bloom, M., Burnell, E.E., Roeder, S.B.W., Valic, M.I.: Nuclear magnetic resonance line shapes in lyotropic liquid crystals and related systems. J. Chem. Phys. 66, 3012–3020 (1977).
Bocian, D.F., Chan, S.I.: NMR studies of membrane structure and dynamics. Annu. Rev. Phys. Chem. 29, 307–335(1978).
Burnett, L.J., Muller, B.H.: Deuteron quadrupole coupling constants in three solid deuterated paraffin hydrocarbons: Ethane-d6, butane-d10, and hexane-d14. J. Chem. Phys. 55, 5829–5831 (1971).
Chan, S.I., Feigenson, G.W., Seiter, C.H.A.: Nuclear relaxation studies of lecithin bilayers. Nature 231, 110–112(1971).
Chan, S.I., Seiter, C.H.A., Feigenson, G.W.: Anisotropic and restricted molecular motion in lecithin bilayers. Biochem. Biophys. Res. Commun. 46, 1488–1492 (1972).
Cullis, R.P.: Lateral diffusion rates of phosphatidylcholine in vesicle membranes: Effects of cholesterol and hydrocarbon phase transitions. FEBS Lett. 70, 223–228 (1976).
Devaux, P., McConnell, H.M.: Lateral diffusion in spin-labeled phosphatidylcholine multilayers. J. Am. Chem. Soc. 94, 4475–4481 (1972).
Edidin, M.: Rotational and translational diffusion in membranes. Annu. Rev. Biophys. Bioeng. 3, 179–201 (1974).
Farrar, T.C., Becker, E.D.: Pulse and Fourier Transform NMR. New York: Academic Press 1971.
Feigenson, G.W., Chan, S.I.: Nuclear magnetic resonance relaxation behavior of lecithin multilayers. J. Am. Chem. Soc. 96, 1312–1319 (1974).
Feigenson, G.W., Meers, P.R., Kingsley, P.B.: NMR observation of gramicidin A in phosphatidylcholine vesicles. Biochim. Biophys. Acta 471, 487–491 (1977).
Finer, E.G.: Calculation of molecular motional correlation times from linewidths in nuclear magnetic resonance spectra of aggregated systems. Effects of particle size on spectra of phospholipid dispersions. J. Magn. Reson. 13, 76–86 (1974).
Finer, E.G., Flook, A.G., Hauser, H.: Mechanism of sonication of aqueous egg yolk lecithin dispersions and nature of the resultant particles. Biochim. Biophys. Acta 260, 49–58 (1972a).
Finer, E.G., Flook, A.G., Hauser, H.: The nature and origin of the NMR spectrum of unsonicated and sonicated aqueous egg yolk lecithin dispersions. Biochim. Biophys. Acta 260, 59–69 (1972b).
Fisher, R.W., James, T.L.: Lateral diffusion of the phospholipid molecules in dipalmitoylphosphati-dylcholine Mayers. An Investigation using nuclear spin relaxation in the rotating frame. Biochemistry 27, 1177–1183 (1978).
Franks, N.P.: Structural analysis of hydrated egg lecithin and cholesterol bilayers. I. X-ray diffraction. J. Mol. Biol. 100, 345–358 (1976).
Gaily, H.-U., Niederberger, W., Seelig, J.: Conformation of the choline head group in bilayers of dipalmitoyl-3-sn-phosphatidylcholine. Biochemistry 14, 3647–3652 (1975).
Gent, M.P.N., Prestegard, J.H.: Comparison of 13C spin lattice relaxation times in phospholipid vesicles and multilayers. Biochem. Biophys. Res. Commun. 58, 549–555 (1974).
Godici, P.E., Landsberger, F.R.: The dynamic structure of lipid membranes. A 13C nuclear magnetic resonance study using spin labels. Biochemistry 13, 362–368 (1974).
Griffin, R.G.: Observation of the effect of water on 31P nuclear magnetic resonance spectra of dipal-mitoyllecithin. J. Am. Chem. Soc. 98, 851–853 (1976).
Habercorn, R.A., Herzfeld, J., Griffin, R.G.: High resolution 31P and 13C nuclear magnetic resonance spectra of unsonicated model membranes. J. Am. Chem. Soc. 100, 1296–1298 (1978).
Horwitz, A.F., Horsley, W.J., Klein, M.P.: Magnetic resonance studies on membrane and model membrane systems: Proton magnetic relaxation rates in sonicated lecithin dispersions. Proc. Natl. Acad. Sci. USA 69, 590–593 (1972).
Horwitz, A.F., Michaelson, D., Klein, M.P.: Magnetic resonance studies on membrane and model membrane systems. III. Fatty acid motions in aqueous lecithin dispersions. Biochim. Biophys. Acta 298, 1–7 (1973).
Hubbell, W.L., McConnell, H.M.: Molecular motion in spin-labeled phospholipids and membranes. J. Am. Chem. Soc. 93, 314–326 (1971).
James, T.L.: Nuclear magnetic Resonance in Biochemistry, Chap. 4. New York: Academic Press 1975.
Jost, P., Waggoner, A.S., Griffin, O.H.: Spin labeling and membrane structure. Structure and function of biological membranes (ed. Rothfield, L.), pp. 83–144. New York: Academic Press 1971.
Khetrapal, C.L., Kunwar, A.C., Tracey, A.S., Diehl, P.: Lyotropic Liquid Crystals. NMR Basic Principles and Progress (eds. Diehl, P., Fluck, E., Kosfeld, R.), Vol. 9, p. 5. Berlin-Heidelberg-New York: Springer 1975.
Kohler, S.J., Klein, M.P.: 31P Nuclear magnetic resonance. Chemical shielding tensors of phospho-rylethanolamine, lecithin, and related compounds: Applications to head-group motion in model membranes. Biochemistry 15, 967–973 (1976).
Kohler, S.J., Klein, M.P.: Orientation of phospholipid head groups in bilayers and membranes determined from 31P nuclear magnetic resonance shielding tensors. Biochemistry 16, 519–526 (1977).
Kroon, P.A., Kainosho, M., Chan, S.I.: The state of molecular motion of cholesterol in lecithin bilayers. Nature 256, 582–584 (1975).
Kroon, P.A., Kainosho, M., Chan, S.I.: Proton magnetic resonance studies of lipid bilayer membranes. Experimental determination of inter- and intramolecular relaxation rates in sonicated phosphatidylcholine bilayer vesicles. Biochim. Biophys. Acta 433, 282–293 (1976).
Lee, A.G., Birdsall, N.J.M., Metcalf, J.C.: Nuclear magnetic relaxation and the biological membrane. Methods Membr. Biol. 2, 1–156 (1974).
Lichtenberg, D., Petersen, N.O., Girardet, J.-L., Kainosho, M., Kroon, P.A., Seiter, C.H.A., Feigen-son, G.W., Chan, S.I.: The interpretation of proton magnetic resonance linewidths for lecithin dispersions. Effect of particle size and chain packing. Biochim. Biophys. Acta 382, 10–21 (1975).
Lindblom, G., Wennerström, H., Arvidson, G., Lindman, B.: Lecithin translational diffusion studied by pulsed nuclear magnetic resonance. Biophys. J. 16, 1287–1295 (1976).
Lindsey, H., Petersen, N.O., Chan, S.I.: Physicochemical characterization of 1,2-di-phytanoyl-sn-glycerol-3-phosphorylcholine in model membrane systems. Biochim. Biophys. Acta 555, 147–167 (1979).
Longmuir, K.J., Dahlquist, F.W.: Direct spectroscopic observation of inner and outer hydrocarbon chains of lipid bilayer vesicles. Proc. Natl. Acad. Sci. USA 73, 2716–2719 (1976).
Longmuir, K.J., Capaldi, R.A., Dahlquist, F.W.: Nuclear magnetic resonance studies of lipid-protein interactions. A model of the dynamics and energetics of phosphatidylcholine bilayers that contain cytochrome c oxidase. Biochemistry 16, 5746–5755 (1977).
McFarland, B.G., McConnell, H.M.: Bent fatty acid chains in lecithin bilayers. Proc. Natl. Acad. Sci. USA 68, 1274–1278(1971).
Michaelson, D.M., Horwitz, A.F., Klein, M.P.: Transbilayer asymmetry and surface homogeneity of mixed phospholipids in cosonicated vesicles. Biochemistry 12, 2637–2645 (1973).
Niederberger, W., Seelig, J.: Phosphorus-31 chemical shift anisotropy in unsonicated phospholipid bilayers. J. Am. Chem. Soc. 98, 3704–3706 (1976).
Petersen, N.O., Chan, S.I.: More on the motional state of lipid bilayer membranes: Interpretation of order parameters obtained from nuclear magnetic resonance. Biochemistry 16, 2657–2667 (1977).
Petersen, N.O., Kroon, P.A., Kainosho, M., Chan, S.I.: Thermal phase transitions in deuterated lecithin bilayers. Chem. Phys. Lipids 14, 343–349 (1975).
Pirs, J., Ukleja, P., Doane, J.W.: NMR in rapidly rotating samples or nematic liquid crystals. Phys. Rev. A 14, 414–423 (1976).
Racker, E.: Reconstitution of cytochrome oxidase vesicles and conferral of sensitivity of energy transfer inhibitors. J. Membr. Biol. 10, 221–235 (1972).
Razin, Shmuel: Reconstitution of biological membranes. Biochim. Biophys. Acta 265, 241–296 (1972).
Saupe, A.: Das Protonenresonanzspektrum von orientiertem Benzol in nematisch-kristallinflüssiger Lösung. Z. Naturforsch. 20a, 572–580 (1965).
Schempp, E., Bray, P.J.: Nuclear quadrupole Resonance spectroscopy. In: Physical Chemistry an Advanced Treatise (eds. Eyring, H., Henderson, D., Jost, W.), Vol. 4, Chap. II. New York-San Francisco: Academic Press 1970.
Schindler, H., Seelig, J.: Deuterium-magnetische Resonanzspektroskopie an spezifisch deuterierten flüssigen Kristallen. Ber. Bunsenges. Phys. Chem. 78, 947–949 (1974).
Seelig, J.: Deuterium magnetic resonance: Theory and application to lipid membranes. Q. Rev. Biophys. 10, 353–418 (1977).
Seelig, J., Gaily, H.-U.: Investigation of phosphatidylethanolamine bilayers by deuterium and phosphorus-31 nuclear magnetic resonance. Biochemistry 15, 5199–5204 (1976).
Seelig, J., Niederberger, W.: Deuterium-labeled lipids as structural probes in liquid crystalline bilayers. A Deuterium magnetic resonance study. J. Am. Chem. Soc. 96, 2069–2072 (1974).
Seelig, J., Seelig, A.: Deuterium magnetic resonance studies of phospholipid bilayers. Biochem. Biophys. Res. Commun. 57, 406–411 (1974).
Seelig, J., Gaily, H.-U., Wohlgemuth, R.: Orientation and flexibility of the choline head group in phosphatidylcholine bilayers. Biochim. Biophys. Acta 467, 109–119 (1977).
Seiter, C.H.A., Chan, S.I.: Molecular motion in lipid bilayers. A nuclear magnetic resonance study. J. Am. Chem. Soc. 95, 7541–7553 (1973).
Sheetz, M.P., Chan, S.I.: Effect of sonication on the structure of lecithin bilayers. Biochemistry 11, 4573–4581 (1972).
Singer, S.J., Nicolson, G.L.: The fluid mosaic model of cell membranes. Science 175, 720–731 (1972).
Stockton, G.W., Polnaszek, C.F., Leitch, L.G., Tulloch, A.P., Smith, I.P.C.: A study of mobility and order in model membranes using 2H NMR relaxation rates and quadrupole splittings of specifically deuterated lipids. Biochem. Biophys. Res. Commun. 60, 844–850 (1974).
Stockton, G.W., Polnaszek, C.F., Tulloch, A.P., Hasan, F., Smith, I.P.C.: Molecular motion in single-bilayer vesicles and multilamellar dispersions of egg lecithin and lecithin-cholesterol mixtures. A deuterium magnetic resonance study of specifically labeled lipids. Biochemistry 15, 954–966 (1976).
Stoll, M.E., Vega, H.J., Vaughn, R.W.: Explicit Demonstration of spinor character for a spin-1/2 nucleus via NMR interferometry. Phys. Rev. A 16, 1521–1524 (1977a).
Stoll, M.E., Vega, A.J., Vaughn, R.W.: Double resonance interferometry: Relaxation times for dipolar forbidden transitions and off resonance effects in an AX spin system. J. Chem. Phys. 67, 2029–2038 (1977b).
Tiddy, G.J.T.: N.M.R. of liquid crystals and micellular solution. Nucl. Magn. Reson. (Specialist Periodical Reports) 4, 233–252 (1975).
Tiddy, G.J.T.: N.M.R. of liquid crystals and micellular solutions. Nucl. Magn. Reson. (Specialist Periodical Reports) 6, 207–232 (1977).
Träuble, H., Sackman, E.: Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. III. Structure of a steroid-lecithin system below and above the lipid-phase transition. J. Am. Chem. Soc. 94, 4499–4510 (1972).
Urbina, J., Waugh, J.S.: Proton-enhanced 13C nuclear magnetic resonance of lipids and biomembranes. Proc. Natl. Acad. Sci. USA 71, 5062–5067 (1974).
Waugh, J.S., Huber, L.M., Haeberlen, U.: Approach to high-resolution NMR in solids. Phys. Rev. Lett. 20, 180–182(1968).
Wennerström, H.: Proton nuclear magnetic resonance lineshapes in lamellar liquid crystals. Chem. Phys. Lett. 18, 41–44 (1973).
Wennerström, H., Lindblom, G.: Biological and model membranes studied by nuclear magnetic resonance of spin one half nuclei. Q. Rev. Biophys. 10, 67–96 (1977).
Wennerström, H., Ulmius, J.: Proton NMR bandshapes in phospholipid bilayer vesicles. J. Magn. Reson. 25, 431–435(1976).
Woessner, D.E.: Spin relaxation in a two spin system undergoing anisotropic reorientation. J. Chem. Phys. 36, 1–4 (1962).
Worcester, D.L., Franks, N.P.: Structural analysis of hydrated egg lecithin and cholesterol bilayers. II. Neutron diffraction. J. Mol. Biol. 100, 359–378 (1976).
Wu, E.-S., Jacobson, K., Papahadjopoulos, D.: Lateral diffusion in phospholipid multilayers measured by fluorescence recovery after photobleaching. Biochemistry 16, 3936–3941 (1977).
Wu, S.H., McConnell, H.M.: Phase separations in phospholipid membranes. Biochemistry 14, 847–854 (1975).
Yeagle, P.L., Hutton, W.C., Huang, C., Martin, R.B.: Structure in the polar head group region of phospholipid bilayers: A 31P {1H} nuclear Overhauser effect study. Biochemistry 15, 2121–2124 (1976).
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Chan, S.I., Bocian, D.F., Petersen, N.O. (1981). Nuclear Magnetic Resonance Studies of the Phospholipid Bilayer Membrane. In: Grell, E. (eds) Membrane Spectroscopy. Molecular Biology Biochemistry and Biophysics, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81537-9_1
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DOI: https://doi.org/10.1007/978-3-642-81537-9_1
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