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Nuclear Magnetic Resonance Studies of the Phospholipid Bilayer Membrane

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Membrane Spectroscopy

Part of the book series: Molecular Biology Biochemistry and Biophysics ((MOLECULAR,volume 31))

Abstract

The cell membrane has been the focus of much recent biochemical and biophysical research, primarily because of its role in cellular phenomena. Numerous efforts have been directed toward determining the motional state of the lipid components of the membrane, the motivation being the contention that the lipid bilayer is the basic matrix in which membrane proteins are embedded to form the biological membrane. As such, it is likely that such diverse phenomena as maintenance of ionic gradients and transmembrane potentials, activities of membrane-bound enzymes, interactions between membrane proteins, transmembrane signal transmission, intercellular communication, and manifestations of cellular development and cell transformation all depend on the structure and fluidity of the lipid bilayer.

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References

  • Abragam, A.: The Principles of Nuclear Magnetism. London: Oxford University Press 1961.

    Google Scholar 

  • Anderson, P.W.: A mathematical model for the narrowing of spectral lines by exchange or motion. J. Phys. Soc. Jpn. 9, 316–339 (1954).

    Article  Google Scholar 

  • Berden, J.A., Barker, R.W., Radda, G.K.: NMR studies on phospholipid bilayers. Some factors affecting lipid distribution. Biochim. Biophys. Acta 375, 186–298 (1975).

    Article  PubMed  CAS  Google Scholar 

  • Bloom, M., Burneil, E.E., Valic, M.I., Weeks, G.: Nuclear magnetic resonance line shapes in lipid bi-layer model membranes. Chem. Phys. Lipids 14, 107–112 (1975).

    Article  PubMed  CAS  Google Scholar 

  • Bloom, M., Burnell, E.E., Roeder, S.B.W., Valic, M.I.: Nuclear magnetic resonance line shapes in lyotropic liquid crystals and related systems. J. Chem. Phys. 66, 3012–3020 (1977).

    Article  CAS  Google Scholar 

  • Bocian, D.F., Chan, S.I.: NMR studies of membrane structure and dynamics. Annu. Rev. Phys. Chem. 29, 307–335(1978).

    Article  CAS  Google Scholar 

  • Burnett, L.J., Muller, B.H.: Deuteron quadrupole coupling constants in three solid deuterated paraffin hydrocarbons: Ethane-d6, butane-d10, and hexane-d14. J. Chem. Phys. 55, 5829–5831 (1971).

    Article  CAS  Google Scholar 

  • Chan, S.I., Feigenson, G.W., Seiter, C.H.A.: Nuclear relaxation studies of lecithin bilayers. Nature 231, 110–112(1971).

    Article  PubMed  CAS  Google Scholar 

  • Chan, S.I., Seiter, C.H.A., Feigenson, G.W.: Anisotropic and restricted molecular motion in lecithin bilayers. Biochem. Biophys. Res. Commun. 46, 1488–1492 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Cullis, R.P.: Lateral diffusion rates of phosphatidylcholine in vesicle membranes: Effects of cholesterol and hydrocarbon phase transitions. FEBS Lett. 70, 223–228 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Devaux, P., McConnell, H.M.: Lateral diffusion in spin-labeled phosphatidylcholine multilayers. J. Am. Chem. Soc. 94, 4475–4481 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Edidin, M.: Rotational and translational diffusion in membranes. Annu. Rev. Biophys. Bioeng. 3, 179–201 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Farrar, T.C., Becker, E.D.: Pulse and Fourier Transform NMR. New York: Academic Press 1971.

    Google Scholar 

  • Feigenson, G.W., Chan, S.I.: Nuclear magnetic resonance relaxation behavior of lecithin multilayers. J. Am. Chem. Soc. 96, 1312–1319 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Feigenson, G.W., Meers, P.R., Kingsley, P.B.: NMR observation of gramicidin A in phosphatidylcholine vesicles. Biochim. Biophys. Acta 471, 487–491 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Finer, E.G.: Calculation of molecular motional correlation times from linewidths in nuclear magnetic resonance spectra of aggregated systems. Effects of particle size on spectra of phospholipid dispersions. J. Magn. Reson. 13, 76–86 (1974).

    Article  CAS  Google Scholar 

  • Finer, E.G., Flook, A.G., Hauser, H.: Mechanism of sonication of aqueous egg yolk lecithin dispersions and nature of the resultant particles. Biochim. Biophys. Acta 260, 49–58 (1972a).

    PubMed  CAS  Google Scholar 

  • Finer, E.G., Flook, A.G., Hauser, H.: The nature and origin of the NMR spectrum of unsonicated and sonicated aqueous egg yolk lecithin dispersions. Biochim. Biophys. Acta 260, 59–69 (1972b).

    PubMed  CAS  Google Scholar 

  • Fisher, R.W., James, T.L.: Lateral diffusion of the phospholipid molecules in dipalmitoylphosphati-dylcholine Mayers. An Investigation using nuclear spin relaxation in the rotating frame. Biochemistry 27, 1177–1183 (1978).

    Article  Google Scholar 

  • Franks, N.P.: Structural analysis of hydrated egg lecithin and cholesterol bilayers. I. X-ray diffraction. J. Mol. Biol. 100, 345–358 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Gaily, H.-U., Niederberger, W., Seelig, J.: Conformation of the choline head group in bilayers of dipalmitoyl-3-sn-phosphatidylcholine. Biochemistry 14, 3647–3652 (1975).

    Article  Google Scholar 

  • Gent, M.P.N., Prestegard, J.H.: Comparison of 13C spin lattice relaxation times in phospholipid vesicles and multilayers. Biochem. Biophys. Res. Commun. 58, 549–555 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Godici, P.E., Landsberger, F.R.: The dynamic structure of lipid membranes. A 13C nuclear magnetic resonance study using spin labels. Biochemistry 13, 362–368 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Griffin, R.G.: Observation of the effect of water on 31P nuclear magnetic resonance spectra of dipal-mitoyllecithin. J. Am. Chem. Soc. 98, 851–853 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Habercorn, R.A., Herzfeld, J., Griffin, R.G.: High resolution 31P and 13C nuclear magnetic resonance spectra of unsonicated model membranes. J. Am. Chem. Soc. 100, 1296–1298 (1978).

    Article  Google Scholar 

  • Horwitz, A.F., Horsley, W.J., Klein, M.P.: Magnetic resonance studies on membrane and model membrane systems: Proton magnetic relaxation rates in sonicated lecithin dispersions. Proc. Natl. Acad. Sci. USA 69, 590–593 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Horwitz, A.F., Michaelson, D., Klein, M.P.: Magnetic resonance studies on membrane and model membrane systems. III. Fatty acid motions in aqueous lecithin dispersions. Biochim. Biophys. Acta 298, 1–7 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Hubbell, W.L., McConnell, H.M.: Molecular motion in spin-labeled phospholipids and membranes. J. Am. Chem. Soc. 93, 314–326 (1971).

    Article  PubMed  CAS  Google Scholar 

  • James, T.L.: Nuclear magnetic Resonance in Biochemistry, Chap. 4. New York: Academic Press 1975.

    Google Scholar 

  • Jost, P., Waggoner, A.S., Griffin, O.H.: Spin labeling and membrane structure. Structure and function of biological membranes (ed. Rothfield, L.), pp. 83–144. New York: Academic Press 1971.

    Google Scholar 

  • Khetrapal, C.L., Kunwar, A.C., Tracey, A.S., Diehl, P.: Lyotropic Liquid Crystals. NMR Basic Principles and Progress (eds. Diehl, P., Fluck, E., Kosfeld, R.), Vol. 9, p. 5. Berlin-Heidelberg-New York: Springer 1975.

    Google Scholar 

  • Kohler, S.J., Klein, M.P.: 31P Nuclear magnetic resonance. Chemical shielding tensors of phospho-rylethanolamine, lecithin, and related compounds: Applications to head-group motion in model membranes. Biochemistry 15, 967–973 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Kohler, S.J., Klein, M.P.: Orientation of phospholipid head groups in bilayers and membranes determined from 31P nuclear magnetic resonance shielding tensors. Biochemistry 16, 519–526 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Kroon, P.A., Kainosho, M., Chan, S.I.: The state of molecular motion of cholesterol in lecithin bilayers. Nature 256, 582–584 (1975).

    Article  PubMed  CAS  Google Scholar 

  • Kroon, P.A., Kainosho, M., Chan, S.I.: Proton magnetic resonance studies of lipid bilayer membranes. Experimental determination of inter- and intramolecular relaxation rates in sonicated phosphatidylcholine bilayer vesicles. Biochim. Biophys. Acta 433, 282–293 (1976).

    CAS  Google Scholar 

  • Lee, A.G., Birdsall, N.J.M., Metcalf, J.C.: Nuclear magnetic relaxation and the biological membrane. Methods Membr. Biol. 2, 1–156 (1974).

    Google Scholar 

  • Lichtenberg, D., Petersen, N.O., Girardet, J.-L., Kainosho, M., Kroon, P.A., Seiter, C.H.A., Feigen-son, G.W., Chan, S.I.: The interpretation of proton magnetic resonance linewidths for lecithin dispersions. Effect of particle size and chain packing. Biochim. Biophys. Acta 382, 10–21 (1975).

    Article  PubMed  CAS  Google Scholar 

  • Lindblom, G., Wennerström, H., Arvidson, G., Lindman, B.: Lecithin translational diffusion studied by pulsed nuclear magnetic resonance. Biophys. J. 16, 1287–1295 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Lindsey, H., Petersen, N.O., Chan, S.I.: Physicochemical characterization of 1,2-di-phytanoyl-sn-glycerol-3-phosphorylcholine in model membrane systems. Biochim. Biophys. Acta 555, 147–167 (1979).

    Article  PubMed  CAS  Google Scholar 

  • Longmuir, K.J., Dahlquist, F.W.: Direct spectroscopic observation of inner and outer hydrocarbon chains of lipid bilayer vesicles. Proc. Natl. Acad. Sci. USA 73, 2716–2719 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Longmuir, K.J., Capaldi, R.A., Dahlquist, F.W.: Nuclear magnetic resonance studies of lipid-protein interactions. A model of the dynamics and energetics of phosphatidylcholine bilayers that contain cytochrome c oxidase. Biochemistry 16, 5746–5755 (1977).

    Article  PubMed  CAS  Google Scholar 

  • McFarland, B.G., McConnell, H.M.: Bent fatty acid chains in lecithin bilayers. Proc. Natl. Acad. Sci. USA 68, 1274–1278(1971).

    Article  PubMed  CAS  Google Scholar 

  • Michaelson, D.M., Horwitz, A.F., Klein, M.P.: Transbilayer asymmetry and surface homogeneity of mixed phospholipids in cosonicated vesicles. Biochemistry 12, 2637–2645 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Niederberger, W., Seelig, J.: Phosphorus-31 chemical shift anisotropy in unsonicated phospholipid bilayers. J. Am. Chem. Soc. 98, 3704–3706 (1976).

    Article  CAS  Google Scholar 

  • Petersen, N.O., Chan, S.I.: More on the motional state of lipid bilayer membranes: Interpretation of order parameters obtained from nuclear magnetic resonance. Biochemistry 16, 2657–2667 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Petersen, N.O., Kroon, P.A., Kainosho, M., Chan, S.I.: Thermal phase transitions in deuterated lecithin bilayers. Chem. Phys. Lipids 14, 343–349 (1975).

    Article  PubMed  CAS  Google Scholar 

  • Pirs, J., Ukleja, P., Doane, J.W.: NMR in rapidly rotating samples or nematic liquid crystals. Phys. Rev. A 14, 414–423 (1976).

    Article  Google Scholar 

  • Racker, E.: Reconstitution of cytochrome oxidase vesicles and conferral of sensitivity of energy transfer inhibitors. J. Membr. Biol. 10, 221–235 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Razin, Shmuel: Reconstitution of biological membranes. Biochim. Biophys. Acta 265, 241–296 (1972).

    PubMed  CAS  Google Scholar 

  • Saupe, A.: Das Protonenresonanzspektrum von orientiertem Benzol in nematisch-kristallinflüssiger Lösung. Z. Naturforsch. 20a, 572–580 (1965).

    Google Scholar 

  • Schempp, E., Bray, P.J.: Nuclear quadrupole Resonance spectroscopy. In: Physical Chemistry an Advanced Treatise (eds. Eyring, H., Henderson, D., Jost, W.), Vol. 4, Chap. II. New York-San Francisco: Academic Press 1970.

    Google Scholar 

  • Schindler, H., Seelig, J.: Deuterium-magnetische Resonanzspektroskopie an spezifisch deuterierten flüssigen Kristallen. Ber. Bunsenges. Phys. Chem. 78, 947–949 (1974).

    Google Scholar 

  • Seelig, J.: Deuterium magnetic resonance: Theory and application to lipid membranes. Q. Rev. Biophys. 10, 353–418 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Seelig, J., Gaily, H.-U.: Investigation of phosphatidylethanolamine bilayers by deuterium and phosphorus-31 nuclear magnetic resonance. Biochemistry 15, 5199–5204 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Seelig, J., Niederberger, W.: Deuterium-labeled lipids as structural probes in liquid crystalline bilayers. A Deuterium magnetic resonance study. J. Am. Chem. Soc. 96, 2069–2072 (1974).

    Article  CAS  Google Scholar 

  • Seelig, J., Seelig, A.: Deuterium magnetic resonance studies of phospholipid bilayers. Biochem. Biophys. Res. Commun. 57, 406–411 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Seelig, J., Gaily, H.-U., Wohlgemuth, R.: Orientation and flexibility of the choline head group in phosphatidylcholine bilayers. Biochim. Biophys. Acta 467, 109–119 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Seiter, C.H.A., Chan, S.I.: Molecular motion in lipid bilayers. A nuclear magnetic resonance study. J. Am. Chem. Soc. 95, 7541–7553 (1973).

    Article  CAS  Google Scholar 

  • Sheetz, M.P., Chan, S.I.: Effect of sonication on the structure of lecithin bilayers. Biochemistry 11, 4573–4581 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Singer, S.J., Nicolson, G.L.: The fluid mosaic model of cell membranes. Science 175, 720–731 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Stockton, G.W., Polnaszek, C.F., Leitch, L.G., Tulloch, A.P., Smith, I.P.C.: A study of mobility and order in model membranes using 2H NMR relaxation rates and quadrupole splittings of specifically deuterated lipids. Biochem. Biophys. Res. Commun. 60, 844–850 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Stockton, G.W., Polnaszek, C.F., Tulloch, A.P., Hasan, F., Smith, I.P.C.: Molecular motion in single-bilayer vesicles and multilamellar dispersions of egg lecithin and lecithin-cholesterol mixtures. A deuterium magnetic resonance study of specifically labeled lipids. Biochemistry 15, 954–966 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Stoll, M.E., Vega, H.J., Vaughn, R.W.: Explicit Demonstration of spinor character for a spin-1/2 nucleus via NMR interferometry. Phys. Rev. A 16, 1521–1524 (1977a).

    Article  CAS  Google Scholar 

  • Stoll, M.E., Vega, A.J., Vaughn, R.W.: Double resonance interferometry: Relaxation times for dipolar forbidden transitions and off resonance effects in an AX spin system. J. Chem. Phys. 67, 2029–2038 (1977b).

    Article  CAS  Google Scholar 

  • Tiddy, G.J.T.: N.M.R. of liquid crystals and micellular solution. Nucl. Magn. Reson. (Specialist Periodical Reports) 4, 233–252 (1975).

    CAS  Google Scholar 

  • Tiddy, G.J.T.: N.M.R. of liquid crystals and micellular solutions. Nucl. Magn. Reson. (Specialist Periodical Reports) 6, 207–232 (1977).

    CAS  Google Scholar 

  • Träuble, H., Sackman, E.: Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. III. Structure of a steroid-lecithin system below and above the lipid-phase transition. J. Am. Chem. Soc. 94, 4499–4510 (1972).

    Article  PubMed  Google Scholar 

  • Urbina, J., Waugh, J.S.: Proton-enhanced 13C nuclear magnetic resonance of lipids and biomembranes. Proc. Natl. Acad. Sci. USA 71, 5062–5067 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Waugh, J.S., Huber, L.M., Haeberlen, U.: Approach to high-resolution NMR in solids. Phys. Rev. Lett. 20, 180–182(1968).

    Article  CAS  Google Scholar 

  • Wennerström, H.: Proton nuclear magnetic resonance lineshapes in lamellar liquid crystals. Chem. Phys. Lett. 18, 41–44 (1973).

    Article  Google Scholar 

  • Wennerström, H., Lindblom, G.: Biological and model membranes studied by nuclear magnetic resonance of spin one half nuclei. Q. Rev. Biophys. 10, 67–96 (1977).

    Article  PubMed  Google Scholar 

  • Wennerström, H., Ulmius, J.: Proton NMR bandshapes in phospholipid bilayer vesicles. J. Magn. Reson. 25, 431–435(1976).

    Google Scholar 

  • Woessner, D.E.: Spin relaxation in a two spin system undergoing anisotropic reorientation. J. Chem. Phys. 36, 1–4 (1962).

    Article  CAS  Google Scholar 

  • Worcester, D.L., Franks, N.P.: Structural analysis of hydrated egg lecithin and cholesterol bilayers. II. Neutron diffraction. J. Mol. Biol. 100, 359–378 (1976).

    Article  PubMed  CAS  Google Scholar 

  • Wu, E.-S., Jacobson, K., Papahadjopoulos, D.: Lateral diffusion in phospholipid multilayers measured by fluorescence recovery after photobleaching. Biochemistry 16, 3936–3941 (1977).

    Article  CAS  Google Scholar 

  • Wu, S.H., McConnell, H.M.: Phase separations in phospholipid membranes. Biochemistry 14, 847–854 (1975).

    Article  CAS  Google Scholar 

  • Yeagle, P.L., Hutton, W.C., Huang, C., Martin, R.B.: Structure in the polar head group region of phospholipid bilayers: A 31P {1H} nuclear Overhauser effect study. Biochemistry 15, 2121–2124 (1976).

    Article  PubMed  CAS  Google Scholar 

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Chan, S.I., Bocian, D.F., Petersen, N.O. (1981). Nuclear Magnetic Resonance Studies of the Phospholipid Bilayer Membrane. In: Grell, E. (eds) Membrane Spectroscopy. Molecular Biology Biochemistry and Biophysics, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81537-9_1

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  • DOI: https://doi.org/10.1007/978-3-642-81537-9_1

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