Intra- and Interprotomeric Interactions between the Catalytic Regions of Aspartokinase I-Homoserine Dehydrogenase I from Escherichia coli K 12

  • Michel Véron
  • Georges N. Cohen
Conference paper

Abstract

Fragments carrying one or the other of the two activities of the bifunctional enzyme aspartokinase I-homoserine dehydrogenase I have been isolated. Their study allows the determination of their localization on the intact subunit. By different approaches, the interactions leading to the regulation of both activities and to the building of the oligomeric structure are put in evidence.

Keywords

Migration Bacillus Polypeptide Glutamine Pseudomonas 

Abbreviations

SDS

sodium dodecyl sulfate

PMB

p-mercuribenzoate

DTNB

5-5’- dithiobis-(2-nitrobenzoic)-acid

PMSF

phenylmethanesulfonylfluoride

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • Michel Véron
    • 1
  • Georges N. Cohen
    • 1
  1. 1.Institut PasteurService de Biochimie CellulaireParisFrance

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