T4-Induced Modification of Escherichia coli RNA Polymerase in vitro

  • Christopher G. Goff
Conference paper


This paper reports an in vitro system derived from T4-infected E. coli B which will modify the α polypeptides of purified E. coli RNA polymerase. The system allows direct investigation of the chemical structure introduced by modification and could provide an assay for purification of the enzyme(s) responsible. Results to date with this in vitro system, plus chemical studies on in vivo-modified α polypeptide, suggest that adenosine diphosphate ribose donated from NAD+ is linked through its terminal ribose to the guanido nitrogen of a unique arginine in the α polypeptide.


Adenine Nucleotide Diphtheria Toxin Dansyl Chloride Corynebacterium Diphtheriae Adenosine Diphosphate Ribose 
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Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • Christopher G. Goff
    • 1
  1. 1.MRC Laboratory of Molecular BiologyCambridgeEngland

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