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Regulation of Mammalian Pyruvate Dehydrogenases

  • Ronald H. Cooper
  • Richard M. Denton
  • Helen T. Pask
  • Philip J. Randle
  • David L. Severson
  • Sue Whitehouse
Conference paper

Abstract

Dichloroacetate and other halogenated carboxylic acids can activate pyruvate dehydrogenase in rat heart and in rat heart mitochondria by effecting conversion of phosphorylated (inactive) pyruvate dehydrogenase into an active (dephosphorylated) form. These compounds are inhibitors of pig heart pyruvate dehydrogenase kinase. Pig heart pyruvate dehydrogenase phosphate phosphatase requires both Mg2+and Ca2+ for activity. Evidence is given for a dual metal requirement of the phosphatase in rat fat cell mitochondria. It is concluded that Mg2+ and Ca2+ are essential cofactors for the phosphatase but that their physiological significance as regulators remains to be established. Pig heart pyruvate dehydrogenase kinase is inhibited by high concentrations of magnesium and by low concentrations of calcium.

Keywords

Pyruvate Dehydrogenase Pyruvate Dehydrogenase Kinase Acetyl Carnitine Pyruvate Oxidation Activate Pyruvate Dehydrogenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • Ronald H. Cooper
    • 1
  • Richard M. Denton
    • 1
  • Helen T. Pask
    • 1
  • Philip J. Randle
    • 1
  • David L. Severson
    • 1
  • Sue Whitehouse
    • 1
  1. 1.Department of Biochemistry, Medical SchoolUniversity WalkBristolEngland

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