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The Heterogeneity of Cell Culture Findings in Patients with Acute Myeloblastic Leukemia

  • D. H. Cowan
  • H. A. Messner
  • J. S. Senn
  • E. A. McCulloch
Part of the Recent Results in Cancer Research / Fortschritte der Krebsforschung / Progrès dans les recherches sur le cancer book series (RECENTCANCER, volume 43)

Abstract

The availability of a colony-forming culture assay that detects early progenitors of granulopoiesis (colony forming units in culture; CFU-C) in human bone marrow cells [1, 2, 4, 5, 8, 9, 10] makes it possible to study cellular events in leukemia. Although the CFU-C culture method does not assay directly for the pluripotent myeloid stem cell, there is ample evidence that cells giving rise to colonies in culture are progeny of the pluripotent stem cell and may provide information about early cellular events (for review, see [7]). Marrow suspensions from adult patients with untreated acute myeloblastic leukemia were examined. The results indicate that there are at least several subtypes; this supports the view that the disease we call acute myeloblastic leukemia (AML) is mechanistically heterogeneous.

Keywords

Acute Myeloid Leukemia Chronic Myeloid Leukemia Blast Cell Acute Myeloblastic Leukemia Princess Margaret Hospital 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Brown, D. H., (III), Carbone, P. P.: In vitro growth of normal and leukemic bone marrow. J. nat. Cancer Inst. 46, 989 (1971).PubMedGoogle Scholar
  2. 2.
    Chervenick, P. A., Boggs, E. R.: Bone marrow colonies: Stimulation in vitro by supernatant from incubated human blood cells. Science 169, 692 (1971).Google Scholar
  3. 3.
    Cowan, D. H., Clarysse, A., Abu-Zahra, H., Senn, J. S., Mcculloch, E. A.: The effect of remission induction in acute myeloblastic leukemia on efficiency of colony formation in culture. Ser. Haemat. V. 2, 105 (1972).Google Scholar
  4. 4.
    Greenberg, P. C., Nichols, W. C., Schrier, S. L.: Granulopoiesis in acute myeloid leukemia and pre-leukemia. New. Engl. J. Med. 284, 1225 (1971).PubMedCrossRefGoogle Scholar
  5. 5.
    Iscove, N. N., Senn, J. S., Till, J. S., Mcculloch, E. A.: Colony formation by normal and leukemic human marrow cells in culture: Effect of conditioned medium from leukocytes. Blood 37, 1 (1971).PubMedGoogle Scholar
  6. 6.
    Iscove, N. N., Messner, H., Till, J. E., Mcculloch, E. A.: Human marrow cells forming colonies in culture: Analysis by velocity sedimentation and suspension culture. Ser. Haemat. V. 2, 37 (1972).Google Scholar
  7. 7.
    Mcculloch, E. A., Till, J. E.: Leukemia considered as defective differentiation: Com-plementary in vivo and culture methods applied to the clinical problem. The Nature of Leukemia, p. 119. Ed.: P. C. Vincent. Sydney, Australia: V. C. N. Blight, Government Printer.Google Scholar
  8. 8.
    Moore, M. A. S., Williams, N., Metcalfe, D.: Characterization of in vitro colony- forming cells in acute and chronic myeloid leukemia. The Nature of Leukemia, p. 135. Ed.: P. C. Vincent. Sydney, Australia: V. C. N. Blight, Government Printer.Google Scholar
  9. 9.
    Pike, B. L., Robinson, W. A.: Human bone marrow colony growth in agar-gel. J. cell, comp. Physiol. 76, 77 (1970).Google Scholar
  10. 10.
    Senn, J. S., Mcculloch, E. A., Till, J. E.: Comparison of colony-forming ability of normal and leukemic human marrow in cell culture. Lancet 1967 II, 597.Google Scholar
  11. 11.
    Stanners, C. P., Eliceiri, G. L., Green, H.: Synthesis of ribosomal RNA in mouse- hamster hybrids. Nature (Lond.) (New Biol.) 250, 52 (1971).Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1973

Authors and Affiliations

  • D. H. Cowan
    • 1
    • 2
    • 3
    • 4
  • H. A. Messner
    • 1
    • 2
    • 3
    • 4
  • J. S. Senn
    • 1
    • 2
    • 3
    • 4
  • E. A. McCulloch
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of MedicinePrincess Margaret HospitalTorontoCanada
  2. 2.Ontario Cancer InstituteTorontoCanada
  3. 3.Sunnybrook HospitalTorontoCanada
  4. 4.University of TorontoTorontoCanada

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