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Material and Methods

  • Marlies Elger
  • Tatsuo Sakai
  • Wilhelm Kriz
Part of the Advances in Anatomy Embryology and Cell Biology book series (ADVSANAT, volume 139)

Abstract

Kidneys of Wistar rats (Ivanovas, Kissleg; 150–300 g BW) were fixed by total body perfusion as described previously (Kaissling and Kriz 1982). After anesthesia with thiobutabarbital (Inactin, 10 mg/100 g BW) or pentobarbital (Nembutal, 0.12 ml/100 g BW i.p.), the abdominal cavity was opened, the aorta clamped beneath the renal arteries, and a catheter was inserted into the abdominal aorta distal to the clamps in a retrograde direction. The catheter contained less than 0.5 ml of a solution prepared of 2 ml of 0.9% sodium chloride and 1 ml of heparin (5,000 IU). After the catheter was connected to the perfusion device, the inferior vena cava was broadly opened, the clamp was removed, and perfusion was started. Without prior flushing, perfusion of the fixative was carried out at a pressure of 180–220 mm Hg for 3 min at room temperature. The fixative contained 1.5% glutaraldehyde, 1.5% formaldehyde, 0.05% picric acid, and 0.08% CaCl2 in 0.1 M cacodylate buffer (pH 7.4). Small pieces of cortical tissue were postfixed in buffered 1% OsO4 dehydrated through a graded series of ethanol and embedded in Epon 812 by standard procedures.

Keywords

Tannic Acid Renal Artery Methylene Blue Abdominal Aorta Inferior Vena 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Marlies Elger
    • 1
  • Tatsuo Sakai
    • 2
  • Wilhelm Kriz
    • 3
  1. 1.Institut für Anatomie und ZellbiologieUniversität HeidelbergHeidelbergGermany
  2. 2.Department of AnatomyJuntendo UniversityBunkyo-ku, Tokyo 113Japan
  3. 3.Institut für Anatomie und ZellbiologieUniversität HeidelbergHeidelbergGermany

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